Biochimica et biophysica acta. General subjects.
Publisher:
Elsevier
Frequency: Monthly
Country: Netherlands
Language: English
Start Year:2017 -
ISSN:
1872-8006 (Electronic)
0304-4165 (Print)
0304-4165 (Linking)
0304-4165 (Print)
0304-4165 (Linking)
Impact Factor
3
2022
| NLM ID: | 101731726 |
| (OCoLC): | 38492156 |
| LCCN: | 2003233285 |
| Classification: | W1 BI65232 |
Transport processes in equine oocytes and ovarian tissue during loading with cryoprotective solutions. Rational design of cryopreservation strategies for oocytes and ovarian cortex tissue requires insights in the rate at which cryoprotective agents (CPA) permeate and concomitant water transport takes place. The aim of the current study was to investigate possible differences in permeation kinetics of different CPAs (i.e., glycerol/GLY, ethylene glycol/EG, dimethyl sulfoxide/DMSO, and propylene glycol/PG), in equine oocytes as well as ovarian tissue. Membrane permeability of oocytes to water (Lp) and to CPAs (Ps) was inferred from video microscopic imaging of oocyte volume responses during perfu...
A highly prevalent equine glycogen storage disease is explained by constitutive activation of a mutant glycogen synthase. Equine type 1 polysaccharide storage myopathy (PSSM1) is associated with a missense mutation (R309H) in the glycogen synthase (GYS1) gene, enhanced glycogen synthase (GS) activity and excessive glycogen and amylopectate inclusions in muscle. Equine muscle biochemical and recombinant enzyme kinetic assays in vitro and homology modelling in silico, were used to investigate the hypothesis that higher GS activity in affected horse muscle is caused by higher GS expression, dysregulation, or constitutive activation via a conformational change. PSSM1-affected horse muscle had significantly higher gly...