Canadian journal of biochemistry.
Discontinued
Publisher:
National Research Council.. Ottawa : National Research Council Of Canada
Frequency: Monthly
Country: Canada
Language: English
Author(s):
National Research Council Canada., National Research Council of Canada.
Start Year:1964 - 1982
Identifiers
| ISSN: | 0008-4018 (Print) 0008-4018 (Linking) |
| NLM ID: | 0421034 |
| (OCoLC): | 02248670 |
| (DNLM): | C03880000(s) |
| Coden: | CJBIAE |
| LCCN: | 65075075 |
| Classification: | W1 CA57 |
The effect of binding ions on the oxidation of horse heart ferrocytochrome c. The research explores how different binding ions affect the oxidation speed of horse heart ferrocytochrome c, a protein, by potassium ferricyanide at a constant ionic strength. Studying the Ion Effect […]
The oxidation of ferrocytochrome c in nonbinding buffer. The apparent equilibrium constant and rate of oxidation was investigated for the reaction of cytochrome c with iron hexacyanide. It was found that if horse heart ferricytochrome c was exposed to ferricyanide (to oxidize traces of reduced protein) the cytochrome subsequently, even after extensive dialysis, had an apparent equilibrium constant different from that of electrodialyzed protein. The effect of ferricyanide ion apparently cannot be removed by ordinary dialysis. The ionic strength dependence of the apparent equilibrium constant and bimolecular oxidation rate constant was measured in the...
Carboxylesterases (EC 3.1.1). Purification and titration of chicken, sheep, and horse liver carboxylesterases. Chicken, sheep, and horse liver carboxylesterases have been purified by procedures involving ammonium sulfate fractionation, ion-exchange chromatography and gel filtration on Sephadex. The actual yields of the procedures described were as follows: chicken, 1 g from 2 kg of liver powder (chloroform-acetone); sheep, 200 mg from 400 g of powder (chloroform-acetone); horse, 230 mg from 800 g of powder (acetone). The purified enzymes are free of non-carboxyl-esterase protein as shown by gel electrophoresis, although they do contain electrophoretic variants. The equivalent weight of the chicken enzy...