Cloning and stem cells.
Discontinued
Publisher:
Mary Ann Liebert, Inc.,
Frequency: Quarterly
Country: United States
Language: English
Start Year:2001 - 2009
Identifiers
| ISSN: | 1536-2302 (Print) 1557-7457 (Electronic) 1536-2302 (Linking) |
| NLM ID: | 101125444 |
| (OCoLC): | 47644130 |
| LCCN: | 2001211763 |
| Classification: | W1 CL933D |
Effect of sperm extract injection volume, injection of PLCzeta cRNA, and tissue cell line on efficiency of equine nuclear transfer. We evaluated the effect of different activation methods on blastocyst development after equine nuclear transfer. All activation treatments were followed by incubation in 2 mM 6-dimethylaminopurine for 4 h. In Experiment 1, reconstructed oocytes were injected with sperm extract for 0.1, 0.2, 0.4, 0.8, or 1.6 sec using a FemtoJet injection device, then treated with ionomycin. The blastocyst rate (9.8%) for 0.1-sec injection was significantly higher than that for 0.2 sec (0%) or 0.8 sec (1.4%). In Experiment 2, injection of murine PLCzeta cRNA before or after ionomycin treatment did not increase ...