Cryobiology.
Periodical
Biology
Cold Temperature
Cryosurgery
Freeze Drying
Hypothermia
Induced
Publisher:
Academic Press. Amsterdam : Elsevier (2000)
Frequency: Six no. a year 2002-
Country: Netherlands
Language: English
Author(s):
Society for Cryobiology.
Start Year:1964 -
ISSN:
0011-2240 (Print)
1090-2392 (Electronic)
0011-2240 (Linking)
1090-2392 (Electronic)
0011-2240 (Linking)
Impact Factor
2.7
2022
| NLM ID: | 0006252 |
| (DNLM): | C53280000(s) |
| (OCoLC): | 00890697 |
| Coden: | CRYBAS |
| Classification: | W1 CR997 |
Effects of phosphatidylserine and cholesterol liposomes on the viability, motility, and acrosomal integrity of stallion spermatozoa prior to and after cryopreservation.
Cryobiology
June 1, 1996
Volume 33, Issue 3 320-329 doi: 10.1006/cryo.1996.0032
Wilhelm KM, Graham JK, Squires EL.Computer-assisted motion analyses (CASA) and flow cytometry were used to evaluate stallion spermatozoa prior to and after cryopreservation. Spermatozoa were pretreated with: (1) Hepes-buffered medium (SHB); (2) phosphatidylserine (PS) liposomes; or (3) liposomes composed of both PS and cholesterol (PSCH) prior to dilution in either SHB or skim milk-egg yolk extender (SMEY). After cooling to 5 degrees C in SHB, PS and PSCH pretreatment (23%). Spermatozoal motion parameters were higher for spermatozoa diluted in SMEY than dilution in SHB. In Experiment 2, motion parameters were compared for sper... In vitro maturation and transmission electron microscopic observation of horse oocytes after vitrification.
Cryobiology
June 1, 1996
Volume 33, Issue 3 300-310 doi: 10.1006/cryo.1996.0030
Hochi S, Kozawa M, Fujimoto T, Hondo E, Yamada J, Oguri N.The study was designed to examine the suitability of immature horse oocytes for vitrification. Immature oocytes derived from slaughtered horse ovaries were transferred to a vitrification solution (EFS; 40% ethylene glycol, 18% Ficoll, and 0.3 M sucrose in modified phosphate-buffered saline) directly (Groups 1 and 4) or were first exposed to 20% ethylene glycol solution for 10 min (Groups 2 and 5) or 20 min (Groups 3 and 6). Oocytes were handled at 20 degrees C (Groups 1, 2, and 3) or 30 degrees C (Groups 4, 5, and 6). After vitrification and warming, their viability was assessed by maturation ... Read More
10
Effect of different protein supplements on motility and plasma membrane integrity of frozen-thawed stallion spermatozoa.
Cryobiology
October 1, 1995
Volume 32, Issue 5 487-492 doi: 10.1006/cryo.1995.1048
Braun J, Hochi S, Oguri N, Sato K, Torres-Boggino F.Three experiments were conducted to evaluate the effect of different macromolecule components (egg yolk, skim milk, and BSA) in a widely employed extender for cryopreservation of horse semen. Spermatozoal motility (MOT) and the percentage of spermatozoa with an intact plasma membrane (IPM) were evaluated in frozen-thawed samples. In the first experiment (four Draft Horse stallions, four ejaculates each) a standard freezing extender containing 20% whole egg yolk was modified by replacing extender components (glucose-EDTA solution, 11% lactose solution) with an increasing volume of a skim milk d... Lipid composition and thermotropic phase behavior of boar, bull, stallion, and rooster sperm membranes.
Cryobiology
April 1, 1992
Volume 29, Issue 2 255-266 doi: 10.1016/0011-2240(92)90024-v
Parks JE, Lynch DV.Composition and thermotropic phase behavior of sperm membrane lipids from species ranging in sensitivity to cold shock were determined. Lipids from whole sperm and sperm plasma membrane were fractionated into neutral lipid, glycolipid, and phospholipid fractions. Compositional analyses were completed for free sterols, phospholipids and phospholipid-bound fatty acids. Phase transition temperatures were determined for phospholipid and glycolipid fractions using differential scanning calorimetry. Cholesterol was the major sterol in sperm lipids of all species. Cholesterol to phospholipid molar ra... Read More
60
An investigation on the use of cryosurgery for treatment of bone spavin, splint, and fractured splint bone injuries in standardbred horses.
Cryobiology
October 1, 1985
Volume 22, Issue 5 468-476 doi: 10.1016/0011-2240(85)90158-0
McKibbin LS, Paraschak DM.Bone spavin, splint, and fractured splint bone injuries have been treated with varying methodologies at Wheatley Hall Farm Equine Clinic. Cryosurgery is the most successful. With cryosurgery the small, pain-producing afferent C fibers are destroyed, and painful neuromas do not return. Injured sites were cryosurgically treated with liquid nitrogen for a double freeze-thaw period of 45 sec. 5 sec, 45 sec. Before and after treatment comparisons were conducted on study standardbreds. In all three injury groups, results showed that the standardbreds tended to race as well or with improved times and... Veterinary cryosurgery.
Cryobiology
June 1, 1982
Volume 19, Issue 3 228-230 doi: 10.1016/0011-2240(82)90147-x
Farris HE, Vestre WA.No abstract available Characteristics and cryopreservation of stallion spermatozoa.
Cryobiology
June 1, 1978
Volume 15, Issue 3 355-357 doi: 10.1016/0011-2240(78)90049-4
Sullivan JJ.No abstract available Tolerance of equine strongylid larvae to desiccation and freezing.
Cryobiology
April 1, 1978
Volume 15, Issue 2 214-218 doi: 10.1016/0011-2240(78)90026-3
Bemrick WJ.No abstract available Lipid composition and thermotropic phase behavior of boar, bull, stallion, and rooster sperm membranes.
Cryobiology
April 1, 1992
Volume 29, Issue 2 255-266 doi: 10.1016/0011-2240(92)90024-v
Parks JE, Lynch DV.Composition and thermotropic phase behavior of sperm membrane lipids from species ranging in sensitivity to cold shock were determined. Lipids from whole sperm and sperm plasma membrane were fractionated into neutral lipid, glycolipid, and phospholipid fractions. Compositional analyses were completed for free sterols, phospholipids and phospholipid-bound fatty acids. Phase transition temperatures were determined for phospholipid and glycolipid fractions using differential scanning calorimetry. Cholesterol was the major sterol in sperm lipids of all species. Cholesterol to phospholipid molar ra... Read More
60
Adding cholesterol to the stallion sperm plasma membrane improves cryosurvival.
Cryobiology
August 24, 2005
Volume 51, Issue 3 241-249 doi: 10.1016/j.cryobiol.2005.07.004
Moore AI, Squires EL, Graham JK.Cryopreservation induces partially irreversible damage to equine sperm membranes. Part of this damage occurs due to membrane alterations induced by the membrane changing from the fluid to the gel-state as the temperature is reduced lower than the membrane transition temperature. One way to prevent this damage is to increase the membrane fluidity at low temperatures by adding cholesterol to the membrane. Different concentrations of cholesterol-loaded-cyclodextrins (CLC) were added to stallion sperm to determine the CLC concentration that optimizes cryosurvival. Higher percentages of motile sper... Read More
24
Use of hypometabolic TRIS extenders and high cooling rate refrigeration for cryopreservation of stallion sperm: presence and sensitivity of 5′ AMP-activated protein kinase (AMPK).
Cryobiology
November 3, 2014
Volume 69, Issue 3 473-481 doi: 10.1016/j.cryobiol.2014.10.008
Córdova A, Strobel P, Vallejo A, Valenzuela P, Ulloa O, Burgos RA, Menarim B, Rodríguez-Gil JE, Ratto M, Ramírez-Reveco A.This study evaluated the effect of the use of hypometabolic TRIS extenders in the presence or the absence of AMPK activators as well as the utilization of high cooling rates in the refrigeration step on the freezability of stallion sperm. Twelve ejaculates were cryopreserved using Botucrio® as a control extender and a basic TRIS extender (HM-0) separately supplemented with 10 mM metformin, 2mM 5-aminoimidazole-4-carboxamide-1-β-D-ribofuranoside (AICAR), 2 mM Adenosine monophosphate (AMP), 40 μM compound C AMPK inhibitor or 2 mM AMP+40 μM compound C. Our results showed that the utilization ... Read More
15
Osmotic tolerance limits and membrane permeability characteristics of stallion spermatozoa treated with cholesterol.
Cryobiology
July 29, 2009
Volume 59, Issue 2 201-206 doi: 10.1016/j.cryobiol.2009.07.009
Glazar AI, Mullen SF, Liu J, Benson JD, Critser JK, Squires EL, Graham JK.Stallion spermatozoa exhibit osmotic damage during the cryopreservation process. Recent studies have shown that the addition of cholesterol to spermatozoal membranes increases the cryosurvival of bull, ram and stallion spermatozoa, but the exact mechanism by which added cholesterol improves cryosurvival is not understood. The objectives of this study were to determine if adding cholesterol to stallion sperm membranes alters the osmotic tolerance limits and membrane permeability characteristics of the spermatozoa. In experiment one, stallion spermatozoa were treated with cholesterol-loaded cycl... Read More
14
Membrane permeability parameters for freezing of stallion sperm as determined by Fourier transform infrared spectroscopy.
Cryobiology
June 8, 2010
Volume 61, Issue 1 115-122 doi: 10.1016/j.cryobiol.2010.06.002
Oldenhof H, Friedel K, Sieme H, Glasmacher B, Wolkers WF.Cellular membranes are one of the primary sites of injury during freezing and thawing for cryopreservation of cells. Fourier transform infrared spectroscopy (FTIR) was used to monitor membrane phase behavior and ice formation during freezing of stallion sperm. At high subzero ice nucleation temperatures which result in cellular dehydration, membranes undergo a profound transition to a highly ordered gel phase. By contrast, low subzero nucleation temperatures, that are likely to result in intracellular ice formation, leave membrane lipids in a relatively hydrated fluid state. The extent of free... Read More
14
Evaluation of growth, viability, and structural integrity of equine endometrial organoids following cryopreservation.
Cryobiology
November 14, 2021
Volume 104 56-62 doi: 10.1016/j.cryobiol.2021.11.003
Thompson RE, Meyers MA, Pukazhenthi BS, Hollinshead FK.Reproductive diseases in mares are a significant cause of subfertility and profound economic loss in the equine industry. Utilizing a 3D in vitro cell culture system that recapitulates the in vivo physiology will reduce time, cost, and welfare concerns associated with in vivo reproductive research in mares. If this 3D model is combined with effective cryopreservation, reproductive research on mares can occur year-round, which is not currently possible in this seasonal species. Endometrial organoids, 3D in vitro cell clusters that exhibit in vivo uterine physiology, have been established in mic... Read More
12
Cryopreservation of epididymal stallion sperm.
Cryobiology
January 8, 2014
Volume 68, Issue 1 91-95 doi: 10.1016/j.cryobiol.2013.12.009
Olaciregui M, Gil L, Montón A, Luño V, Jerez RA, Martí JI.Any event that makes semen collection or mating impossible, such as death, castration, or injury, may terminate a stallion's breeding career. Fortunately, stallion sperm which are capable of fertilization can be harvested from the epididymis, and frozen for future use. However, the fertility of frozen-thawed epididymal sperm has been found to be lower than that of ejaculated sperm. Therefore, this study aimed to optimize the fertility of frozen epididymal stallion sperm by investigating the effects of different cryoprotectants and freezing protocols on sperm quality. Dimethylformamide was test... Read More
10
First results about ProAKAP4 concentration in stallion semen after cryopreservation in two different freezing media.
Cryobiology
July 21, 2021
Volume 102 133-135 doi: 10.1016/j.cryobiol.2021.07.009
Blommaert D, Sergeant N, Delehedde M, Donnay I, Lejeune JP, Franck T, Serteyn D.The quality of fresh or thawed sperm in stallions has been generally determined by the viability and total and progressive motility of the sperm. Today, the expression of ProAKAP4, a protein present in the flagellum of spermatozoa, appears to be an innovative and relevant functional marker to assess semen quality and male fertility. This study aims to compare the concentration of ProAKAP4 in the semen from 5 stallions frozen with two different extenders immediately after thawing (T0) and 4 h post-thawing (T4). Viability, total and progressive motility were measured in parallel. Significant di... Read More
10
In vitro maturation and transmission electron microscopic observation of horse oocytes after vitrification.
Cryobiology
June 1, 1996
Volume 33, Issue 3 300-310 doi: 10.1006/cryo.1996.0030
Hochi S, Kozawa M, Fujimoto T, Hondo E, Yamada J, Oguri N.The study was designed to examine the suitability of immature horse oocytes for vitrification. Immature oocytes derived from slaughtered horse ovaries were transferred to a vitrification solution (EFS; 40% ethylene glycol, 18% Ficoll, and 0.3 M sucrose in modified phosphate-buffered saline) directly (Groups 1 and 4) or were first exposed to 20% ethylene glycol solution for 10 min (Groups 2 and 5) or 20 min (Groups 3 and 6). Oocytes were handled at 20 degrees C (Groups 1, 2, and 3) or 30 degrees C (Groups 4, 5, and 6). After vitrification and warming, their viability was assessed by maturation ... Read More
10
Improved cryosurvival of stallion spermatozoa after colloid centrifugation is independent of the addition of seminal plasma.
Cryobiology
February 15, 2018
Volume 81 145-152 doi: 10.1016/j.cryobiol.2018.01.009
Al-Essawe EM, Johannisson A, Wulf M, Aurich C, Morrell JM.Addition of seminal plasma (SP) prior to cryopreservation may influence stallion sperm cryosurvival. The objective of this study was to investigate the addition of pooled SP from "good" or "bad" freezer stallions to spermatozoa selected by single layer centrifugation (SLC) prior to cryopreservation on post-thaw sperm quality. Semen from 12 stallions was collected; 5 mL was frozen as control (C) and the remainder was processed by SLC to remove SP and was divided into three aliquots: i) SLC sample without SP (SLC); ii) SLC plus pooled SP from "good freezer" stallions (SLC-GF); iii) SLC plus po... Impact of supplementation of semen extender with antioxidants on the quality of chilled or cryopreserved Arabian stallion spermatozoa.
Cryobiology
October 4, 2017
Volume 79 14-20 doi: 10.1016/j.cryobiol.2017.10.001
Ghallab AM, Shahat AM, Fadl AM, Ayoub MM, Moawad AR.The aim of the present study was to evaluate the effects of supplementation of semen extender with various non-enzymatic antioxidants on the quality of cooled or cryopreserved Arabian stallion spermatozoa. Semen collected from four pure Arabian stallions was centrifuged at 600g for 15 min. Spermatozoa were then diluted in INRA-82 extender supplemented with bovine serum albumin (BSA; 0, 10, 15 and 20 mg/mL) or trehalose (0, 75, 100 and 150 mM) or zinc sulphate (0, 100, 150 and 200 μM). The diluted semen was then either cooled at 5 °C or cryopreserved in 0.5-ml plastic straws. After cooli... Improved sperm cryosurvival in diluents containing amides versus glycerol in the Przewalski’s horse (Equus ferus przewalskii).
Cryobiology
February 6, 2014
Volume 68, Issue 2 205-214 doi: 10.1016/j.cryobiol.2014.01.013
Pukazhenthi BS, Johnson A, Guthrie HD, Songsasen N, Padilla LR, Wolfe BA, Coutinho da Silva M, Alvarenga MA, Wildt DE.Two studies were conducted to understand sperm cryosensitivity in an endangered equid, the Przewalski's horse (Equus ferus przewalski), while testing the cryoprotectant ability of formamides. The first assessed the toxicity of permeating cryoprotectants (glycerol, methylformamide [MF] and dimethylformamide [DMF]) to Przewalski's horse spermatozoa during liquid storage at 4°C. The second examined the comparative influence of three diluents (with or without formamides) on cryosurvival of sperm from the Przewalski's versus domestic horse. When Przewalski's horse spermatozoa were incubated at 4°... Effects of different concentration and combinations of cryoprotectants on sperm quality, functional integrity in three Indian horse breeds.
Cryobiology
December 18, 2018
Volume 86 52-57 doi: 10.1016/j.cryobiol.2018.12.005
Soni Y, Talluri TR, Kumar A, Ravi SK, Mehta JS, Tripathi BN.Traditionally Glycerol (Gly) is being used as major cryoprotectant and its toxicity could be a reason for the variation on stallion sperm freezability and fertility. In an effort to minimize Gly toxicity alternative cryoprotective agents like Di-methyl Formamide (DMF) have been investigated. The effect of the cryoprotectant and dose of cryoprotective agent varies from breed to breed and also from stallion to stallion within the same breed. Considering these factors a study was designed to study the effects of Gly and DMF at different concentrations and combinations on the plasma membrane, acro... Blastocyst development after intracytoplasmic sperm injection of equine oocytes vitrified at the germinal-vesicle stage.
Cryobiology
February 13, 2017
Volume 75 52-59 doi: 10.1016/j.cryobiol.2017.02.004
Canesin HS, Brom-de-Luna JG, Choi YH, Ortiz I, Diaw M, Hinrichs K.We evaluated the meiotic and developmental competence of GV-stage equine oocytes vitrified under different conditions. In a preliminary study, using dimethyl sulfoxide (D), ethylene glycol (EG) and sucrose (S) as cryoprotectants, the maturation rate was higher for cumulus-oocyte complexes (COCs) held overnight before vitrification (37%) than for those vitrified immediately (14%; P < 0.05). Thereafter, all COCs were held overnight before vitrification. In Experiment 1 we compared 1 min (1m) and 4 min (4m) exposure to vitrification and warming solutions; oocytes that subsequently matured wer... Vitrification of germinal-vesicle stage equine oocytes: Effect of cryoprotectant exposure time on in-vitro embryo production.
Cryobiology
January 3, 2018
Volume 81 185-191 doi: 10.1016/j.cryobiol.2018.01.001
Canesin HS, Brom-de-Luna JG, Choi YH, Pereira AM, Macedo GG, Hinrichs K.Previous studies have found low rates of blastocyst development (0-11%) after vitrification of germinal vesicle (GV)-stage equine oocytes. In this study, we systematically evaluated a short (non-equilibrating) system for GV-stage oocyte vitrification. In Exp. 1, we assessed oocyte volume in cumulus-oocyte complexes (COCs) exposed to components of a short protocol, using 2% each of ethylene glycol and propylene glycol in the first solution (VS1); 17.5% of each plus 0.3 M trehalose in the second solution (VS2); and fetal bovine serum as the base medium. Based on the time to oocyte minimum volu... Embryo development after vitrification of immature and in vitro-matured equine oocytes.
Cryobiology
January 19, 2020
Volume 92 251-254 doi: 10.1016/j.cryobiol.2020.01.014
Angel D, Canesin HS, Brom-de-Luna JG, Morado S, Dalvit G, Gomez D, Posada N, Pascottini OB, Urrego R, Hinrichs K, Velez IC.Effects of meiotic stage and cumulus status on development of equine oocytes after vitrification was evaluated. Immature oocytes with corona radiata (IMM); in vitro-matured oocytes with corona radiata (MAT CR+); and in vitro-matured oocytes denuded of cumulus (MAT CR-) were vitrified using the Cryotech® method. Warming medium was equilibrated either in 5% CO2 or Air. IMM oocytes underwent in vitro maturation after warming. Recovery, survival, and maturation rates, and cleavage and blastocyst rates after ICSI, were evaluated. Recovery was higher for oocytes warmed in CO2- than Air-equilibrated... Substitution of egg yolk by a cyclodextrin-cholesterol complex allows a reduction of the glycerol concentration into the freezing medium of equine sperm.
Cryobiology
December 11, 2015
Volume 72, Issue 1 27-32 doi: 10.1016/j.cryobiol.2015.11.008
Blommaert D, Franck T, Donnay I, Lejeune JP, Detilleux J, Serteyn D.The aim of this work was to completely replace the egg yolk a classical diluent for freezing equine semen by a cyclodextrin-cholesterol complex. At the same time, the reduction in the glycerol content used for cryopreservation and the incubation time between sperm and the freezing media were evaluated. Horse ejaculates were frozen with four different freezing extenders: a frozen reference medium (IF) containing egg yolk and 2.5% glycerol and media without egg yolk but supplemented with 1.5 mg 2-hydroxypropyl-beta-cyclodextrin cholesterol (HPβCD-C) complex and containing either 1% (G1), 2% (G... Dimethyl sulfoxide maintains structure and function of cryopreserved equine endometrial explants.
Cryobiology
October 15, 2019
Volume 91 90-96 doi: 10.1016/j.cryobiol.2019.10.006
Thompson RE, Johnson AK, Prado TM, Premanandan C, Brown ME, Whitlock BK, Pukazhenthi BS.Availability of viable frozen-thawed endometrial tissues could facilitate detailed studies into physiologic and disease processes influencing the endometrium. This study was designed to investigate the cryosurvival of equine endometrial tissue. Previous studies in the human and horse have focused on cryopreservation of dissociated endometrial cells. To our knowledge, there are no studies on cryopreservation of endometrial explants. Our objectives were to 1) determine the influence of differing concentrations of the permeating cryoprotectant dimethyl sulfoxide (MeSO) on viability, structural in... Effect of trehalose- and sucrose-based extenders on equine sperm quality after vitrification: Preliminary results.
Cryobiology
December 8, 2017
Volume 80 62-69 doi: 10.1016/j.cryobiol.2017.12.002
Pérez-Marín CC, Requena FD, Arando A, Ortiz-Villalón S, Requena F, Agüera EI.There has been a lack of research into equine sperm vitrification to date, but studies of other species suggest it may have significant potential. To evaluate the impact of various cryoprotectant agents (CPA) and vitrification on equine sperm quality, a controlled study was carried out. A total of 12 ejaculates were subjected to exposure to CPA and vitrification. Sperm was diluted in a range of CPA: fresh, control (BSA), sucrose (0.15M, 0.3M and 0.5M), trehalose (0.15M, 0.3M and 0.5M) and the combination of sucrose and trehalose (M1: 0.15M sucrose+0.5M trehalose; M2: 0.5M sucrose+0.15M trehalo... Effects of phosphatidylserine and cholesterol liposomes on the viability, motility, and acrosomal integrity of stallion spermatozoa prior to and after cryopreservation.
Cryobiology
June 1, 1996
Volume 33, Issue 3 320-329 doi: 10.1006/cryo.1996.0032
Wilhelm KM, Graham JK, Squires EL.Computer-assisted motion analyses (CASA) and flow cytometry were used to evaluate stallion spermatozoa prior to and after cryopreservation. Spermatozoa were pretreated with: (1) Hepes-buffered medium (SHB); (2) phosphatidylserine (PS) liposomes; or (3) liposomes composed of both PS and cholesterol (PSCH) prior to dilution in either SHB or skim milk-egg yolk extender (SMEY). After cooling to 5 degrees C in SHB, PS and PSCH pretreatment (23%). Spermatozoal motion parameters were higher for spermatozoa diluted in SMEY than dilution in SHB. In Experiment 2, motion parameters were compared for sper... Effects of cholesterol-loaded cyclodextrins on the quality of frozen-thawed equine epididymal sperm.
Cryobiology
June 12, 2011
Volume 63, Issue 2 90-95 doi: 10.1016/j.cryobiol.2011.05.006
Pamornsakda T, Pojprasath T, Suwimonteerabutr J, Tharasanit T.Equine epididymal sperm are known to be severely sensitive to cryopreservation, in terms of sperm quality and pregnancy rate. The objective of this study was to examine the effects of cholesterol loaded cyclodextrins (CLCs) on the quality of stallion epididymal sperm during cryopreservation. In experiment I, sperm were treated with different concentrations of CLCs: (1) 0mg (control), (2) 1.5mg, (3) 3mg, and (4) 6 mg per 120 × 10(6) sperm. The sperm viability and amount of cholesterol were determined at 15, 30 and 45 min after CLC treatment using viability markers (Ethidium homodimer-1 and Cal... Effects of Spermine and Spermidine supplemented extenders on post-thaw Spermatological Parameters in Stallion Semen Cryopreservation.
Cryobiology
March 29, 2021
Volume 100 72-76 doi: 10.1016/j.cryobiol.2021.03.008
Olğaç KT, Akçay E.In this study, the effects of polyamines, Spermine and Spermidine, on long-term preservation and post-thaw spermatological parameters were evaluated. Moreover, determination of the most suitable polyamine and its dose that can be added to standard extenders were aimed. Four adult Arabian stallions were used in the study. Five ejaculates were collected from each of four stallions via artificial vagina two days interval. Each ejaculate was divided into 13 aliquots. INRA96 (95,5%), egg yolk (2%), and glycerol (2,5%) were used as a control extender. Extenders of experimental groups were prepared w... Consequences of adding gum Arabic as a cryoprotectant on motility and viability of frozen stallion semen.
Cryobiology
October 3, 2017
Volume 79 21-28 doi: 10.1016/j.cryobiol.2017.09.008
Ali M, Musa MM, Alfadul S, Al-Sobayel K.A trial was conducted to check effect of adding gum Arabic (GA) instead of egg yolk (EY) as a cryoprotectant for stallion sperm. Two experiments were designed; experiment I tested adding 3 levels of nonheated GA (i.e., 3, 6 and 9 g/100 mL diluents) in HF-20 extender. However, in experiment II the same levels were tested except that GA was heated at 80 °C for 60 min. HF-20 containing 10% of EY was used as control. In experiment I, sperm frozen in HF-20 containing nonheated GA exhibited lower percentages of motile sperm, progressively motile sperm and sperm with intact plasma membranes, vit... Equine sperm post-thaw evaluation after the addition of different cryoprotectants added to INRA 96® extender.
Cryobiology
July 2, 2014
Volume 69, Issue 1 144-148 doi: 10.1016/j.cryobiol.2014.06.008
Álvarez C, Gil L, González N, Olaciregui M, Luño V.The rise of assisted reproduction techniques in equine medicine has fostered investigations that seek to optimize methods to increase fertility rates. Since cryopreservation continues to give low values of viability in stallions, the handling and preservation of the sperm is of vital importance. This reduction of fertility makes it essential for farmers to find new options that ensure reliability in the use of these techniques. The main objective of this study was to assess the effect of INRA 96® (manufactured commercial extender for cooling of Equine semen) as an extender for cryopreservatio... Computer simulations to determine the efficacy of different genome resource banking strategies for maintaining genetic diversity.
Cryobiology
August 2, 2002
Volume 44, Issue 2 122-131 doi: 10.1016/s0011-2240(02)00013-5
Harnal VK, Wildt DE, Bird DM, Monfort SL, Ballou JD.Genome resource banks (GRBs) and assisted reproductive techniques are increasingly recognized as useful tools for the management and conservation of biodiversity, including endangered species. Cryotechnology permits long-term storage of valuable genetic material. Although, the actual application to endangered species management requires technical knowledge about sperm freezing and thawing, a systematic understanding of the quantitative impacts of various germ plasm storage and use scenarios is also mandatory. In this study, various GRB strategies were analyzed using the historical data from th... Curcumin-loaded niosomal nanocarriers offer a promising approach to improve quality characteristics, apoptotic gene expression, and flow cytometry assessments of stallion spermatozoa after thawing.
Cryobiology
December 29, 2024
Volume 118 105188 doi: 10.1016/j.cryobiol.2024.105188
Nasiri-Foomani N, Hassani S, Najafi M, Samadi F.The optimization of cryopreservation media to reduce oxidative damage on post-thaw spermatozoa is crucial. This research aimed to assess the antioxidant properties of curcumin-loaded niosomal nanocarriers (CurLNN) on the functional characteristics, the relative expression of apoptotic genes, and flow cytometry assessments of apoptotic-like changes, reactive oxygen species production (ROS), mitochondrial membrane potential, and chromatin integrity in stallion spermatozoa following thawing. Twenty-five ejaculates were diluted in INRA96 freezing media supplemented with 20 μM of either curcumin ... A preliminary study on the use of jenny colostrum to improve quality in extenders for freezing donkey semen.
Cryobiology
January 24, 2019
Volume 87 110-114 doi: 10.1016/j.cryobiol.2019.01.009
Álvarez C, Luño V, González N, Gil L.Sperm quality in donkeys (Equus asinus) after freezing thawing is still considered lower than that from other animals, including horses. The aim of this study was to test a new freezing extender supplemented with jenny colostrum on donkey sperm. After thawing, we evaluated sperm motility by means of computer-assisted analysis, viability by SYBR-14 and propidium iodide (PI), membrane functional integrity by HOS-test and acrosome integrity by isothiocyanate conjugated with peanut agglutinin (FITC-PNA) and PI. Ejaculates were collected from five fertile Donkeys. Sperm samples were pooled, diluted... Collection, processing and freezing of equine bone marrow cells.
Cryobiology
June 20, 2017
Volume 78 95-100 doi: 10.1016/j.cryobiol.2017.06.006
Bastos FZ, Barussi FCM, Santi TF, Vieira BP, Senegaglia AC, Cruz FF, Michelotto PV.There is no consensus on aspects of equine bone marrow collection and processing. The study aimed to describe the collection of large volumes of bone marrow from horses of advanced age, with emphasis on bone marrow mononuclear cells (BMMCs) recovery and viability after cryopreservation. Fourteen horses, aged 3-24 years, were divided into three experiments. E1 studied the feasibility of collecting 200 mL from the sternums of horses of advanced age; E2 examined the number of cells obtained from the first and last syringe of each puncture; and E3 investigated the influence of heparin concentrati... l-carnitine enhances the kinematics and protects the sperm membranes of chilled and frozen-thawed Peruvian Paso horse spermatozoa.
Cryobiology
March 7, 2024
104884 doi: 10.1016/j.cryobiol.2024.104884
Palacios P, Peláez G, Soria M, Méndez S, Galarza-Álvarez L, Dorado J, Santiago-Moreno J, Galarza DA.l-carnitine (LC) transports fatty acids to the mitochondria for energy production, reducing lipid availability for peroxidation through β-oxidation. This research examines the effect of LC supplementation to two skimmed milk-based extenders on the cryosurvival of chilled (5ºC) and frozen-thawed Peruvian Paso horse spermatozoa. An initial experiment determined the optimal LC concentration (0, 1, 5, 10, 25, and 50 mM) when added to INRA-96® and UHT (skimmed milk + 6% egg yolk) extenders, using nine ejaculates from three stallions chilled for up to 96 h. Subsequently, the effect of 25 mM... Veterinary cryosurgery.
Cryobiology
June 1, 1982
Volume 19, Issue 3 228-230 doi: 10.1016/0011-2240(82)90147-x
Farris HE, Vestre WA.No abstract available