Cytotechnology.
Publisher:
Martinus Nijhoff Publishers,. Hingham, MA : Kluwer Academic Publishers (1988)
Frequency: Nine no. a year, 1991-
Country: United States
Language: English
Start Year:1987 -
ISSN:
0920-9069 (Print)
1573-0778 (Electronic)
0920-9069 (Linking)
1573-0778 (Electronic)
0920-9069 (Linking)
Impact Factor
2.2
2022
| NLM ID: | 8807027 |
| (DNLM): | SR0060925(s) |
| (OCoLC): | 17645742 |
| Coden: | CYTOER |
| LCCN: | sn 88026784 |
| Classification: | W1 CY88 |
Phenotypical and functional characteristics of mesenchymal stem cells derived from equine umbilical cord blood. Mesenchymal stem cells (MSCs) offer promise as therapeutic aid in the repair of tendon and ligament injuries in race horses. Fetal adnexa is considered as an ideal source of MSCs due to many advantages, including non-invasive nature of isolation procedures and availability of large tissue mass for harvesting the cells. However, MSCs isolated from equine fetal adnexa have not been fully characterized due to lack of species-specific markers. Therefore, this study was carried out to isolate MSCs from equine umbilical cord blood (UCB) and characterize them using cross-reactive markers. The plastic...
Expression of scleraxis and tenascin C in equine adipose and umbilical cord blood derived stem cells is dependent upon substrata and FGF supplementation. Recovery from tendon injury is based on long periods of rest, which results in sub-optimal repair, often replacing tendon with fibrocartilage scar tissue. Recently, the use of stem cells in equine tendon repair has been attempted with variable success. The objective of this work was to determine the expression of scleraxis (scx) and tenascin C (TnC), two markers of tenocytes, in adipose (AdMSC) and umbilical cord blood (UCB) stem cells during culture on various substrata and in response to fibroblast growth factor (FGF) treatment. Equine UCB and AdMSC were cultured on gelatin-coated plasticwar...
Isolation and differentiation potential of an equine amnion-derived stromal cell line. Stem cells represent an important tool in veterinary therapeutic field such as tissue engineering. In the present study, equine amnion-derived mesenchymal stromal cells were investigated for applications in veterinary science as an alternative source to bone marrow mesenchymal stem cells and adipose stem cells. Amnion stromal cells isolation and characterization protocol is described; the in vitro cell growth rate was calculated by measuring viable cell number over 20 days. The expression of stem cell markers such as Oct-4, Nanog, Sox-2 and CD105 was assessed by retrotranscription quantitativ...