Development.
Publisher:
Company of Biologists,. Cambridge Eng : Company Of Biologists Limited
Frequency: Twenty four no. a year
Country: England
Language: English
Author(s):
Company of Biologists.
Start Year:1987 -
ISSN:
0950-1991 (Print)
1477-9129 (Electronic)
0950-1991 (Linking)
1477-9129 (Electronic)
0950-1991 (Linking)
Impact Factor
4.6
2022
| NLM ID: | 8701744 |
| (DNLM): | SR0058569(s) |
| (OCoLC): | 15088415 |
| Coden: | DEVPED |
| Classification: | W1 DE997NR |
Establishment and characterization of equine mammary organoids using a method translatable to other non-traditional model species. Mammary organoid (MaO) models are only available for a few traditional model organisms, limiting our ability to investigate mammary gland development and cancer across mammals. This study established equine mammary organoids (EqMaOs) from cryopreserved mammary tissue, in which mammary tissue fragments were isolated and embedded into a 3D matrix to produce EqMaOs. We evaluated viability, proliferation and budding capacity of EqMaOs at different time points during culture, showing that although the number of proliferative cells decreased over time, viability was maintained and budding increased....
Invasive equine trophoblast expresses conventional class I major histocompatibility complex antigens. Monoclonal antibodies and alloantisera were used in an indirect immunohistochemical assay to determine the expression of class I and class II Major Histocompatibility Complex (MHC) antigens by equine placental cells and the endometrial tissues at the fetal-maternal interface. MHC class I antigens were expressed at high density on the surface of the trophoblast cells of the chorionic girdle at days 32-36, just prior to their invasion of the endometrium. The mature gonadotrophin-secreting cells of the endometrial cups, which are derived from the chorionic girdle cells, had greatly reduced levels...