DNA.
Discontinued
Publisher:
M.A. Liebert,. New York Ny : Mary Ann Liebert
Frequency: Ten no. a year
Country: United States
Language: English
Start Year:1981 - 1989
Identifiers
| ISSN: | 0198-0238 (Print) 0198-0238 (Linking) |
| NLM ID: | 8302432 |
| (OCoLC): | 06177540 |
| (DNLM): | D17135000(s) |
| Coden: | DNAADR |
| Classification: | W1 DN125 |
Molecular cloning and expression in Escherichia coli of equine type I interferons. Using human interferon-alpha 2 (IFN-alpha 2) and IFN-beta DNA to probe an equine genomic library we isolated recombinant phages containing genes for equine interferon-alpha (EqIFN-alpha), interferon-beta (EqIFN-beta), and interferon-omega (EqIFN-omega). Sequence and hybridization analyses of these genes reveal that the equine genome contains gene families of each of these three type I interferon classes. The mature proteins of EqIFN-alpha are 71-77% homologous to human IFN-alpha polypeptides, and, when expressed in E. coli, possess antiviral activity on both equine and human cells. By contrast...