Iranian journal of biotechnology.
Publisher:
National Research Center for Genetic Engineering and Biotechnology,
Frequency: Quarterly
Country: Iran
Language: English
Author(s):
National Research Center for Genetic Engineering and Biotechnology (Iran),
Start Year:2003 -
ISSN:
2322-2921 (Electronic)
1728-3043 (Print)
1728-3043 (Linking)
1728-3043 (Print)
1728-3043 (Linking)
Impact Factor
1.3
2022
| NLM ID: | 101276796 |
| (OCoLC): | 402781974 |
| LCCN: | 2018243176 |
| Classification: | W1 |
Cloning and Expression of Iranian Turkmen-thoroughbred Horse Follicle Stimulating Hormone in Pichia pastoris. Follicle stimulating hormone (FSH) plays an essential role in reproductive physiology and follicular development. Objective: A new variant of the equine () gene was cloned, sequenced, and expressed in ) GS115 yeast expression system. Methods: The full-length cDNAs of the and chains were amplified by reverse transcription polymerase chain reaction (RT-PCR) using the total RNA isolated from an Iranian Turkmen-thoroughbred horse's anterior pituitary gland. The amplified chains were cloned into the pPIC9 vector and transferred into The secretion of recombined eFSH using expression system was...