Journal of biochemical and biophysical methods.
Discontinued
Publisher:
Elsevier/North-Holland Biomedical Press.
Frequency: Six no. a year, -
Country: Netherlands
Language: English
Start Year:1979 - 2008
Identifiers
| ISSN: | 0165-022X (Print) 1872-857X (Electronic) 0165-022X (Linking) |
| NLM ID: | 7907378 |
| (DNLM): | J14230000(s) |
| (OCoLC): | 04936947 |
| LCCN: | 80640152 |
| Classification: | W1 JO562 |
In situ zymography: topographical considerations. In situ gelatin zymography is a simple technique providing valuable information about the cellular and tissue localization of gelatinases. Until recently, the use of this technique has been confined to soft, relatively homogeneous tissue. In this report in situ zymography has been utilized to assess the sub-lamellar location of gelatinases in the hard, semi-keratinized epidermal layer and the adjacent soft connective tissue matrix of the dermis of the equine hoof. We show that alterations in the orientation at which the tissue is dipped and withdrawn from the emulsion cause profound alteration...