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The Journal of cell biology.
Publisher:
Rockefeller University Press
Frequency: Monthly, 2017-
Country: United States
Language: English
Author(s):
Rockefeller Institute., American Society for Cell Biology.
Start Year:1962 -
ISSN:
0021-9525 (Print)
1540-8140 (Electronic)
0021-9525 (Linking)
1540-8140 (Electronic)
0021-9525 (Linking)
Impact Factor
7.8
| NLM ID: | 0375356 |
| (DNLM): | J15460000(s) |
| (OCoLC): | 01390147 |
| Coden: | JCLBA3 |
| LCCN: | 56058208 |
| Classification: | W1 JO578B |
The resolution of mixtures of viable mammalian cells into homogeneous fractions by zonal centrifugation. Large-scale separation of mixtures of mammalian cells was obtained with the A-1X zonal centrifuge rotor and density gradients consisting of Ficoll dissolved in modified Eagle's MEM suspension-culture medium. The cells remained viable as tested by plating efficiency or by motility observed with time-lapse photography. Rabbit thymocyte and HeLa cell mixtures were separated with 99 and 89 per cent purity, respectively. Mixtures of thymocytes and suspension-cultured, human acute leukemia cells (Roswell Park strain LKID) were separated with 93 and 91% purity, respectively. HeLa cells were isolated ...
