Journal of chemical education.
Publisher:
Division of Chemical Education, American Chemical Society.. Tucson Az : American Chemical Society. Division Of Chemical Education
Frequency: Monthly
Country: United States
Language: English
Author(s):
American Chemical Society. Division of Chemical Education.
Start Year:1924 -
ISSN:
0021-9584 (Print)
0021-9584 (Linking)
0021-9584 (Linking)
Impact Factor
3
2022
| NLM ID: | 341042 |
| (DNLM): | J15660000(s) |
| (OCoLC): | 01754494 |
| Coden: | JCEDA8 |
| Classification: | W1 JO581 |
Case-Study Investigation of Equine Maternity via PCR-RFLP: A Biochemistry Laboratory Experiment. A simple and robust biochemistry laboratory experiment is described that uses restriction fragment length polymorphism (RFLP) of polymerase chain reaction (PCR) products to verify the identity of a potentially valuable horse. During the first laboratory period, students purify DNA from equine samples and amplify two loci of mitochondrial DNA. During the second laboratory period, students digest PCR products with restriction enzymes and analyze the fragment sizes through agarose gel electrophoresis. An optional step of validating DNA extracts through realtime PCR can expand the experiment to th...