Journal of photochemistry and photobiology. B, Biology.
Publisher:
Elsevier Sequoia,
Frequency: Twelve no. a year 2001-
Country: Switzerland
Language: English
Author(s):
European Society for Photobiology.
Start Year:1987 -
ISSN:
1011-1344 (Print)
1873-2682 (Electronic)
1011-1344 (Linking)
1873-2682 (Electronic)
1011-1344 (Linking)
Impact Factor
5.4
2022
| NLM ID: | 8804966 |
| (DNLM): | SR0061068(s) |
| (OCoLC): | 16877735 |
| LCCN: | 88648144 |
| Classification: | W1 JO832BH |
Fluorescence investigations on choline phospholipid binding and chemical unfolding of HSP-1/2, a major protein of horse seminal plasma. Seminal fibronectin type-II (Fn-II) proteins interact with choline phospholipids present on the sperm plasma membrane and play a crucial role in sperm capacitation. Crystal structure of phosphorylcholine (PrC) complex of PDC-109, the major bovine Fn-II protein, together with fluorescence spectroscopic studies has shown that tryptophan residues are crucial for its specific interaction with choline phospholipids. In the present study, the heterogeneity and microenvironment of tryptophan residues in HSP-1/2, a major protein of horse seminal plasma (which is homologous to PDC-109) were investigate...
Local electrostatic potentials in pyridoxal phosphate labelled horse heart cytochrome c. The present work shows the application of an optical label pyridoxal phosphate (PLP) for the experimental determination of local electrostatic potentials in singly substituted cytochromes c modified by pyridoxal phosphate at Lys 79 (PLP-Lys-79-cyt.c) or at Lys 86 (PLP-Lys-86-cyt.c). PLP has also been used to calculate the pKa values of all ionizable groups and the electrostatic potentials in the modified proteins and to analyse their properties. The experimental pKa values for the pyridine nitrogen and phenolic hydroxyl of the bound label were obtained from pH-dependent absorbance and fluoresc...