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Journal of reproduction and fertility. Supplement.
Discontinued
Publisher:
Blackwell Scientific Publications for Journals of Reproduction and Fertility,. Cambridge, UK : Journals of Reproduction and Fertility, Ltd.
Frequency: Irregular
Country: England
Language: English
Author(s):
Society for the Study of Fertility.
Start Year:1966 - 2001
Identifiers
| ISSN: | 0449-3087 (Print) 0449-3087 (Linking) |
| NLM ID: | 0225652 |
| (DNLM): | J37020000(s) |
| (OCoLC): | 01639439 |
| Coden: | JRFSAR |
| LCCN: | sn 82004517 |
| Classification: | W1 JO868KA |
Factors affecting phagocytosis of bacteria by neutrophils in the mare’s uterus. Ten mares, 5 resistant and 5 susceptible to bacterial endometritis, were examined for differences pertaining to the efficiency of phagocytosis of bacteria by neutrophils in the uterus. An assay for chemiluminescence was used to evaluate various schemes of opsonization and relate them to phagocytic rate. 123I-labelled albumin was used to measure protein migration to the inoculated uterus. Neutrophil numbers moving to the uterine lumen were determined. Before inoculation resistant mares were found to have substances in their uterine secretions that opsonized bacteria, resulting in effective phag...
Chemical composition of the spinal cord in the normal developing fetus and in the premature foal. The lipid content of spinal cord, expressed as a percentage of adult values, was considerably higher for newborn foals than for several other species and traces of esterified cholesterol (type A) were only rarely present in horse fetal cord (from 270 days gestational age onwards). This suggested that, at birth, the spinal cord is neurochemically more 'mature' in the horse than in cattle, sheep and pigs. Data for premature foals revealed no lipid abnormality suggestive of myelin immaturity or degeneration.
Relaxin activity in foaling mares. Plasma relaxin concentrations were measured hourly by radio immunoassay in 4 pregnant mares from 11 days before until 4 days after natural foaling. Pre-partum levels ranged from 4 to 7 ng/ml without any surge until the second stage of labour when they increased rapidly to about 11 ng/ml. In 3 of these mares, relaxin activity declined immediately after the expulsion of the placenta and was below detectable levels within 36 h. In the other mare relaxin activity did not fall until after the mechanical removal of the placenta 7 h after foaling. Eight mares were induced to foal by the administratio...
Results of concurrent bacteriological and cytological examinations of the endometrium of mares in routine stud farm practice 1978-1981. The results of 4024 concurrent endometrial swab and smear tests taken from maiden, barren and post-parturient mares during early oestrus, before coitus demonstrated the practical value of these tests in routine stud farm practice. The use of cytological examinations provided a more direct diagnostic test for acute endometritis and this increased the accuracy of interpretation of the bacteriological findings.
Social organization of feral horses. The basic social unit in feral horses is the family group consisting of one stallion, one to a few unrelated mares and their foals. Surplus stallions associate in bachelor groups. Stallions are instrumental in bringing mares together in a unit which then persists even without a stallion. The similarity of social organization in populations living in a variety of different habitats indicates that feral horses have reverted to the habits of their wild ancestors, and that domestication has had no influence on this basic behavioural feature.
Fertility of frozen equine semen. Semen of 16 stallions collected by the fractionated method and frozen in liquid nitrogen was used to inseminate 175 mares of different ages and in various reproductive conditions. Pregnancy was recorded in 91 mares of which 72 delivered a foal. Pregnancy followed by resorption occurred in another 10 mares and 9 aborted. The best results were obtained in the young primiparous and in older mares inseminated in the oestrous cycle that followed the post-partum oestrus. Overall, 64% of mares became pregnant and 56% gave birth to a living foal. The highest occurrence of fetal death and resorption we...
Factors affecting testis weight in normal and cryptorchid horses. Testes were collected from normal and cryptorchid horses of a variety of breeds and ages and weighed after dissection from the epididymis. Scrotal testes grow little until the second winter of life and little thereafter, although a nearly mature body weight is reached by the end of the first winter. Scrotal testes in unilateral cryptorchids tend to be larger than those of normal stallions, sometimes exceedingly so, although occasional small scrotal testes are recorded. Inguinal testes show some tendency to grow during the second winter but the data are difficult to analyse beyond that age beca...
Genetics of Standardbred stallion reproductive performance. Reproductive performance of 10 Standardbred stallions was related to the probability that the embryo resulting from a given mating would be heterozygous for transferrin or plasma esterase. Fertility, measured by foaling rate per insemination or by foaling rate per year, showed a highly significant regression on the probability of offspring heterozygosity for transferrin and, to lesser extent, for esterase. Substantial differences between stallions in the slope of the regression line and no deficiency of foals homozygous for either protein suggests that the relationship to fertility is indirect...
Fetal loss and the effect of stress on plasma progestagen levels in pregnant Thoroughbred mares. The depressing effect on plasma progestagen levels of pregnant mares subjected to specific stressful conditions such as severe pain, infectious diseases, emotional disturbances and exogenous corticosteroids are described. It is concluded that the detrimental effect of stress, evident from its negative influence on plasma progestagen concentrations, could play a major role in the occurrence of pregnancy failure in the Thoroughbred mare.
Maternal anti-fetal cytotoxic antibody responses of equids during pregnancy. The maternal immunological response to the developing equine fetus was investigated in 69 pregnancies of various genotypes. In normal intraspecies horse pregnancy (N = 16), 94% of mares produced strong cytotoxic antibody responses to paternal histocompatibility antigens which were first detectable between 44 and 70 days after ovulation. In all other types of pregnancy examined (intraspecies donkey, N = 19; interspecies mule, N = 6; interspecies hinny, N = 2; extraspecies horse-in-donkey, N = 3; and extraspecies donkey-in-horse, N = 21), antibody production was observed less frequently, and som...
Effects of washing on the bacterial flora of the stallion’s penis. Six stallions were subjected to extensive cleansing of the penis and prepuce with water, Ivory Soap and water, or Betadine surgical scrub and water. The stallions were all washed for 14 days, and then allowed 14 days respite. This pattern of washing and resting was repeated consecutively. Swabs were taken from all 7 stallions twice weekly and semen was collected once a week for bacteriological examination. All forms of cleansing altered the bacterial flora of the stallion's penis; the Ivory Soap tended to encourage the replacement of the normal flora with coliform organisms, while Betadine fav...
Is Rhodococcus equi a soil organism? A total of 189 isolates of Rhodococcus equi and related organisms and 16 marker strains representing the genera Rhodococcus and Corynebacterium were screened for 160 unit characters in a numerical taxonomic study. Analysis of the data indicated that R. equi forms a relatively homogeneous cluster distinctly separated from the recognized species of Rhodococcus and Corynebacterium (sensu stricto). Other members of the genus Rhodococcus are soil organisms and R. equi appears to fit into the genus on ecological as well as taxonomic grounds. It seems unlikely that R. equi could be a gastrointestinal...
Androgens, behaviour and fertility control in feral stallions. This field study of feral stallions in Montana and Idaho examines and correlates the seasonal pattern of plasma androgens and specific sociosexual behaviour and reports the effect of a long-acting androgenic steroid on this behaviour and on fertility. Plasma testosterone was measured by competitive protein binding assay in samples obtained by jugular venepuncture from captured animals. In samples taken from 34 sexually mature stallions in 6 different months during the year, a definite seasonal pattern in testosterone was present, with a peak in May (3.04 +/- 0.63 ng/ml) and a nadir in December...
Ultrasonic echography in the mare. Ultrasonic echography in the mare allows pregnancy diagnosis as early as Day 14 after ovulation. In the 1980 and 1981 breeding seasons, a total of 7438 examinations of 4688 mares were performed on several farms with the same apparatus. The accuracy of positive pregnancy diagnosis was estimated to be greater than 95% and of non-pregnancy diagnosis greater than 84%. When abnormal pictures of vesicles are found, it has to be decided whether it is a conceptus or a cystic structure. Most small vesicles do not develop, whereas large ones, deformed shapes, presence of some echoes, or a division insid...
Ovarian activity in the mare between latitude 15 degrees and 22 degrees N. Ovarian activity, as measured by the presence of small (10-15 mm diam.), medium (15-30 mm) and large (greater than 30 mm) follicles, corpora haemorrhagica and corpora lutea (CL), was determined in a total of 3584 ovaries during 1 year using post-mortem specimens. There were significant correlations of the incidence of ovulated follicles (ovulations), large and medium follicles (P less than 0 . 001) and of the mean number of small follicles (P less than 0 . 05) per mare with respect to season. The yearly averages of ovulated, large and medium follicles were 30 . 1, 13 . 9 and 73 . 9%, respectiv...
Recovery of contagious equine metritis organisms and development of lesions in experimental infection of mares. Twenty-three of 24 mares were infected experimentally with contagious equine metritis organisms by intrauterine inoculation, and killed 2-116 days later. From mares killed within 14 days after infection the organism could be recovered from many sites in the uterus, and most sites in the cervix, a few sites in the vagina and oviduct and from one clitoral sinus. At this time the endometrial folds were swollen and there were 10-20 ml of fluid in the uterus. In mares killed after 14 days, the organism was recovered from the ovarian surface (1 mare), oviduct (4 mares), uterus (2 mares) and the clit...
Effect of prolonged administration of anabolic and androgenic steroids on reproductive function in the mare. Administration to mares of the anabolic steroid, methandriol, at the maximum recommended dose (300 mg every 3 weeks) for 1 1/2 years had no effect on reproductive characteristics except for suppression of GnRH-induced LH release and a tendency to suppress basal LH levels and the height of the ovulatory LH surge. A 4-fold increase in dosage caused marked suppression of basal LH, the LH surge, and GnRH-induced LH release. Other reproductive responses were minimally affected. There were no behavioural effects, and no changes in weight occurred when mares were compared with matched controls. Small...
Radioimmunoassay and in-vitro bioassay of serum LH throughout the equine oestrous cycle. Mares were bled once daily throughout a cycle, or 3 times daily from the first day of oestrus to the 2nd day after ovulation. LH was measured by heterologous radioimmunoassay and by an in-vitro bioassay based on LH-stimulated testosterone production by mouse Leydig cells. The patterns of bio- and immuno-active LH during the oestrous cycle were similar but not identical, so that in both groups of mares the ratio of biological: immunological (B:I) activity during the LH surge was significantly higher before than after ovulation (P less than 0 . 001). Considerable individual variation in cycle me...
Response of the adrenal cortex to tetracosactrin (ACTH1-24) in the premature and full-term foal. The changes in plasma cortisol concentration in the immediate postnatal period were examined in 3 groups of newborn foals and the response of the adrenal cortex to exogenous ACTH1-24 (tetracosactrin) was tested in 2 of these groups. In full-term Thoroughbred and Pony foals a rise in plasma cortisol occurred between 0 and 30 min after birth, whereas no significant cortisol changes could be detected within 2 h of birth in the group of prematurely delivered foals. These differences in plasma cortisol between term and premature foals were accompanied by differences in blood pH and lymphocyte and n...
Effect of pregnancy and collection technique on prostaglandin F in the uterine lumen of Pony mares. Uterine flushings were obtained through the cervix (Method A) and through the wall of the uterus after hysterectomy (Method B) of ovariectomized Pony mares after s.c. injection of oestrogen for 1 week and progesterone for 2 weeks (Exp. 1). Non-pregnant and pregnant mares were flushed by Method A on Day 14 after ovulation and the flushings compared with those of non-pregnant mares injected i.v. with flunixen meglumine, a prostaglandin synthetase inhibitor, shortly before flushing (Exp. 3). Uterine flushings were also collected by Methods A and B from non-pregnant and pregnant Pony mares on Day ...
Progesterone, prostaglandin F-2 alpha, PMSG and oestrone sulphate during early pregnancy in the mare. Blood samples from 4 mares during the late luteal phase, oestrus, early pregnancy and up to about 150 days of gestation were analysed for 15-keto-13,14-dihydroprostaglandin F-2 alpha (PGFM), progesterone, PMSG and oestrone sulphate by radioimmunoassays. During the late luteal phase, at the time of corpus luteum regression and decreasing progesterone levels, PGFM peaks were recorded. During early pregnancy (i.e. from mating and up to about Day 30) no such peaks were detected. After mating the progesterone levels increased and remained high throughout the observation period. During the oestrous ...
Importance of testicular hormones in maintaining the annual pattern of LH secretion in the male horse. Serum LH was measured by radioimmunoassay in 5 long-term (greater than or equal to 3 years) castrated male horses bled at 30-min intervals for 2 h twice a month from March to February. There was no significant effect of month on LH levels; however, mean levels were significantly lower in spring than autumn (P less than 0.05). By contrast, stallions in the same environment showed a markedly seasonal pattern of LH secretion, with LH rising at the onset of the breeding season to reach levels in late spring 3-4 times those in early winter. Despite differences in seasonal patterns of secretion, ann...
Changes in equine follicular aromatase activity during transition from winter anoestrus. Follicular aromatase activity during sexual resurgence after the winter anoestrus was investigated in 3 groups of 5 Pony mares. Group ET was studied during the early transition period, Group LT in late transition and Group C in full breeding condition. Granulosa and theca cells were incubated for 3 h with 3H-labelled androstenedione or progesterone. Analysis of the free oestrogenic products was by high performance liquid chromatography (HPLC) and recrystallization revealed highly variable oestrogen production in both cell types from mares in all 3 groups. Only oestrone and oestradiol peaks wer...
Pancreatic beta cell function in the neonatal foal. Plasma concentrations of insulin and glucose were measured in Pony and Thoroughbred foals at birth and at intervals thereafter for up to 7 days. The plasma concentrations of insulin in foals of both breeds at birth were 11.2 +/- 2.5 microU/ml (N = 6) and 13.5 +/- 1.5 microU/ml (N = 16) respectively. These values were similar to those obtained for foals in utero but were significantly less than those in adult animals. There was little variation in the plasma concentrations of insulin and glucose during the first 2 h of life and both concentrations tended to rise in the next 24-48 h. Although th...
An investigation of sperm migration into the oviducts of the mare. A total of 23 mares were inseminated once within 0-6 h after clinical detection of ovulation, 14 with fresh and 9 with deep-frozen semen containing 0.1 x 10(9) to 4.7 x 10(9) motile spermatozoa. Within these two groups, the mares were slaughtered 2, 4 or 6 h after insemination and their genital tracts removed. The utero-tubal junction, isthmus and ampulla ipsilateral to the ovary in which ovulation occurred were flushed separately for sperm recovery. In 1 or 2 mares of each group, the uterine horn and corpus uteri, the cervix and vagina were also flushed. Tissue samples were collected from the...
Fertility of stallions with abnormalities of the sperm acrosome. During a 2-year period, 7 stallions were identified as having ejaculated spermatozoa characterized by a high incidence (27-74%) of acrosomal abnormalities. The most frequent abnormality of the acrosome was the 'knobbed sperm' defect which was observed in nigrosin--eosin, Giemsa, and haematoxylin and eosin stained semen smears under light microscopy, in buffered formal--saline and in glutaraldehyde-fixed wet mounts under phase contrast and differential interference microscopy, and in glutaraldehyde-fixed spermatozoa with electron microscopy and scanning electron microscopy. The defect was visib...
Studies on the composition and antibacterial activity of uterine fluid from mares. Forty uterine fluid samples were obtained during oestrus and dioestrus of successive cycles from 4 mares classified as resistant to bacterial infection of the uterus. The flushings were assayed for peroxidase activity and ability to influence phagocytosis by polymorphonuclear neutrophils. Uterine fluid volumes and protein concentrations and serum oestrogen values were similar during oestrus and dioestrus. Serum progesterone concentrations were depressed during oestrus. Equine neutrophils adherent to glass slides were exposed to a genital strain of Streptococcus zooepidemicus suspended in salin...
Pituitary and chorionic gonadotrophic control of ovarian function during early pregnancy in equids. Concentrations of FSH, LH, chorionic gonadotrophin (CG) and progesterone were measured during early pregnancy in mares and donkeys carrying normal intraspecies and transferred extraspecies conceptuses. A secondary rise in progesterone concentrations occurred in normal intraspecies horse and donkey pregnancy soon after the appearance of CG but FSH concentrations continued to fluctuate and were not influenced by CG production. In donkeys carrying transferred horse conceptuses FSH concentrations fell sharply and progesterone concentrations rose steeply, coincidental with the appearance of abnorma...
Dehydroepiandrosterone synthesis by the fetal foal and its importance as an oestrogen precursor. The gonads of the fetal horse were found to be relatively devoid of 3 beta-hydroxysteroid dehydrogenase and other enzymes which metabolize dehydroepiandrosterone (DHA). In short-term in-vitro incubation experiments fetal liver converted DHA to the potential equilin precursor, 7 alpha-hydroxy DHA. DHA was converted to oestrone when incubated with extracts of horse placenta but 7 alpha-hydroxy DHA was not converted to equilin. Levels of DHA measured in peripheral blood of mares throughout pregnancy paralleled those of equilin and oestrone, and DHA concentrations fell rapidly after fetal gonadect...
Comparison of the interaction of equine LH and human chorionic gonadotrophin to equine testicular receptors. Human chorionic gonadotrophin (hCG) can be used to study horse luteinizing hormone (LH) receptors in stallion testicular tissue. hCG was more stable than horse LH during radioiodination when compared by their abilities to bind to testicular receptor sites. During incubation, neither hormone lost binding activity at 4 degrees C. Horse LH lost binding activity during incubation at 25 degrees C and both hormones lost binding activity at 37 degrees C. Both hormones bound to the same receptor sites which are specific for the hormones. The receptor sites were not degraded when incubated at 4 degrees...
