Mechanisms of ageing and development.
Publisher:
Elsevier Sequoia.. Limerick : Elsevier Science Ireland
Frequency: Sixteen no. a year
Country: Ireland
Language: English
Start Year:1972 -
ISSN:
0047-6374 (Print)
1872-6216 (Electronic)
0047-6374 (Linking)
1872-6216 (Electronic)
0047-6374 (Linking)
Impact Factor
5.3
2022
| NLM ID: | 0347227 |
| (DNLM): | M08860000(s) |
| (OCoLC): | 02239471 |
| Coden: | MAGDA3 |
| LCCN: | 73641085 |
| Classification: | W1 ME106A |
Age-related changes in genomic stability of horses. Recently, the old horse has been proposed as a model to study telomere-dependent senescence, immunosenescence and inflamm-aging. In the present paper, we used 80 Hucul and Anglo-Arabian horses divided into 3 age groups (juvenile, adult, old) to evaluate age-dependent changes at the genomic and DNA level and in cell proliferative potential. The level of positive TUNEL cells (both apoptotic and with DNA fragmentation), oxidative DNA damage (8-oxoG immunostaining), sister chromatid exchange and bleomycin-induced chromatid breaks were significantly increased in the combined old group compared to t...
Advanced age in horses affects divisional history of T cells and inflammatory cytokine production. A number of model systems have been employed to investigate age-associated changes in immune function. The purpose of the current study was to characterize senescent T cells and to investigate the inflamm-aging phenomenon both in vitro and in vivo using the old horse as a model. We examined whether decreased T cell proliferation induced by Con A is caused by increased apoptosis. We also utilized intracellular CFSE to analyze changes within each round of cell proliferation, in particular cytokine production. Intracellular staining with flow cytometry, RT-PCR, and ELISA were used to measure pro-...
Equine telomeres and telomerase in cellular immortalisation and ageing. To determine the role of telomeres in cellular ageing in equids, we analysed telomere lengths in peripheral blood derived DNA samples from a panel of donkeys (Equus asinus) ranging from 2 to 30 years of age. The average telomere lengths ranged from 7 to 21 kbp and a statistically significant inverse correlation between telomere lengths and donor age was demonstrated. Similarly, telomere lengths in primary fibroblasts isolated from a horse (Equus equus) demonstrated telomeric loss with in vitro ageing when cultured to senescence. We extended this study to evaluate activity of the enzyme telomer...