Methods in cell science : an official journal of the Society for In Vitro Biology.
Discontinued
Publisher:
Kluwer Academic Publishers,
Frequency: Quarterly
Country: Netherlands
Language: English
Author(s):
Society for In Vitro Biology.
Start Year:1995 - 2004
Identifiers
| ISSN: | 1381-5741 (Print) 1573-0603 (Electronic) 1381-5741 (Linking) |
| NLM ID: | 9515839 |
| (DNLM): | SR0084502(s) |
| (OCoLC): | 32655453 |
| LCCN: | sn 95023259 |
| Classification: | W1 JO966JD |
Thoughts on the source of tissue on subsequent cell culture success. This paper describes attempts to initiate equine adipocyte cultures from necropsy cases with varying intervals from time of death to isolation and culture. Equine adipocytes were isolated from 21 necropsy cases, regardless of the interval from time after death to establishment in primary ceiling cultures. However, while all cultures produced adipocytes, only 2 attempts to produce long-term equine adipocyte cultures from the subcutaneous rump fat depots were successful and not contaminated. Findings from these experiments indicate that it is possible to collect and culture equine adipocytes fro...