Mitochondrial DNA.
Discontinued
Publisher:
Informa Healthcare
Frequency: Bimonthly
Country: England
Language: English
Start Year:2008 - 2015
Identifiers
| ISSN: | 1940-1736 (Print) 1940-1744 (Electronic) 1940-1736 (Linking) |
| NLM ID: | 101487102 |
| (OCoLC): | 173300868 |
| LCCN: | 2007213338 |
| Classification: | W1 DN125K |
Primer effect in the detection of mtDNA heteroplasmy: insights from horse Cytochrome b gene. Heteroplasmy, the presence of bi-allelic mtDNA types within an individual, has been previously detected in the D-loop region and Cytochrome b gene (Cytb) of mitochondrial DNA using PCR-RFLP. However, heteroplasmy was absent in thousands of equine mtDNA sequences deposited in GenBank. To address whether heteroplasmy widely exists in mitochondria of Chinese indigenous horses, we generated the data set of the target sites in Cytb region with Sanger sequencing and PCR-RFLP method as well. In this study, 23 heteroplasmic individuals were detected in 430 Chinese local horses. Both site and length he...
Mitochondrial and pedigree analysis in Przewalski’s horse populations: implications for genetic management and reintroductions. Przewalski's horses have been imported from the western zoos to China since 1985. Yet the genetic diversity in China's populations has not been studied, thus lacking of such knowledge inevitably affects this population's management. The aim of this study was to assess genetic diversity in Chinese population of Przewalski's horses via mitochondrial DNA (mtDNA) control region and pedigree analysis. Methods: Two captive and one reintroduced populations were examined based on mitochondrial DNA control region variation via fecal sampling from 2010 to 2012, together with pedigree analysis. Results: ...