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Molecular biotechnology.

Periodical
Biotechnology
Molecular Biology
Publisher:
Humana Press,. [Cham] : Springer
Frequency: Bimonthly
Country: Switzerland
Language: English
Start Year:1994 -
ISSN:
1073-6085 (Print)
1559-0305 (Electronic)
1073-6085 (Linking)
Impact Factor
2.6
2022
NLM ID:9423533
(DNLM):SR0078694(s)
(OCoLC):29487200
Coden:MLBOEO
LCCN:sn 93002050
Classification:W1 MO196DB
Identification of Reference Genes for Analysis of microRNA Expression Patterns in Equine Chorioallantoic Membrane and Serum.
Molecular biotechnology    December 5, 2017   Volume 60, Issue 1 62-73 doi: 10.1007/s12033-017-0047-2
Dini P, Loux SC, Scoggin KE, Esteller-Vico A, Squires EL, Troedsson MHT, Daels P, Ball BA.MicroRNAs (miRNAs) have important posttranscriptional regulatory abilities, and there is considerable interest in evaluating their expression patterns in different pathophysiological states. The most common method of quantifying miRNA expression is quantitative reverse transcription PCR; however, the identification of tissue-specific and species-specific reference miRNA is a prerequisite for miRNA expression analysis. Currently, no reference genes have been described for evaluating miRNA expression in equine serum and chorioallantoic membrane (CAM) during pregnancy. The aim of the present stud...