Pharmacology research & perspectives.
Publisher:
John Wiley & Sons Ltd, British Pharmacological Society and American Society for Pharmacology and Experimental Therapeutics,
Country: United States
Language: English
Author(s):
British Pharmacological Society,, American Society for Pharmacology and Experimental Therapeutics,
Start Year:2013 -
ISSN:
2052-1707 (Electronic)
2052-1707 (Linking)
2052-1707 (Linking)
Impact Factor
2.6
2023
| NLM ID: | 101626369 |
| (OCoLC): | 875081105 |
| LCCN: | 2014243347 |
Equine hepatocytes: isolation, cryopreservation, and applications to in vitro drug metabolism studies. Despite reports of the successful isolation of primary equine hepatocytes, there are no published data regarding the successful cryopreservation of these isolated cells. In this study, a detailed description of the procedures for isolation, cryopreservation, and recovery of equine hepatocytes are presented. Furthermore, the intrinsic clearance (Cl) and production of metabolites for three drugs were compared between freshly isolated and recovered cryopreserved hepatocytes. Primary equine hepatocytes were isolated using a two-step collagenase perfusion method, with an average cell yield of 2.47Â...