Revue canadienne de biologie
Discontinued
Publisher:
L'Université de Montréal,. Montreal : Presses De L Universite De Montreal
Frequency: Quarterly
Country: Canada
Language: English
Author(s):
Université de Montréal., Société de Biologie de Montréal.
Start Year:1942 - 1981
Identifiers
| ISSN: | 0035-0915 (Print) 0035-0915 (Linking) |
| NLM ID: | 8214595 |
| (DNLM): | R41800000(s) |
| (OCoLC): | 01764018 |
| Coden: | RCBIAS |
| Classification: | W1 RE745N |
Effects of dilution rates, animal species and instruments on the spectrophotometric determination of sperm counts. Using semen from bull, boar and stallion as well as different spectrophotometers, we established the calibration curves relating the optical density of a sperm sample to the sperm count obtained on the hemacytometer. The results show that, for a given spectrophotometer, the calibration curve is not characteristic of the animal species we studied. The differences in size of the spermatozoa are probably too small to account for the anticipated specificity of the calibration curve. Furthermore, the fact that different dilution rates must be used, because of the vastly different concentrations of ...