Topic:Magnetic Cell Sorting
Magnetic Cell Sorting (MCS) is a technique used to separate specific cell types from a heterogeneous cell population in horses. This method employs magnetic particles coated with antibodies that bind to specific cell surface markers, allowing for the isolation of target cells when subjected to a magnetic field. MCS is utilized in equine research to study various cell populations, such as immune cells, stem cells, and other cell types relevant to veterinary medicine and equine biology. This technique aids in understanding cellular functions, disease mechanisms, and potential therapeutic applications. This page compiles peer-reviewed research studies and scholarly articles that explore the methodology, applications, and outcomes of magnetic cell sorting in equine research.
The tendon interfascicular basement membrane provides a vascular niche for CD146+ cell subpopulations. The interfascicular matrix (IFM; also known as the endotenon) is critical to the mechanical adaptations and response to load in energy-storing tendons, such as the human Achilles and equine superficial digital flexor tendon (SDFT). We hypothesized that the IFM is a tendon progenitor cell niche housing an exclusive cell subpopulation. Immunolabelling of equine superficial digital flexor tendon was used to identify the interfascicular matrix niche, localising expression patterns of CD31 (endothelial cells), Desmin (smooth muscle cells and pericytes), CD146 (interfascicular matrix cells) and LA...
Generation and characterization of monoclonal antibodies to equine NKp46. The immunoreceptor NKp46 is considered to be the most consistent marker of NK cells across mammalian species. Here, we use a recombinant NKp46 protein to generate a panel of monoclonal antibodies that recognize equine NKp46. The extracellular region of equine NKp46 was expressed with equine IL-4 as a recombinant fusion protein (rIL-4/NKp46) and used as an immunogen to generate mouse monoclonal antibodies (mAbs). MAbs were first screened by ELISA for an ability to recognize NKp46, but not IL-4, or the structurally related immunoreceptor CD16. Nine mAbs were selected and were shown to recognize ...