Topic:Methylome
The equine methylome refers to the complete set of DNA methylation patterns in the horse genome. DNA methylation is an epigenetic mechanism involving the addition of a methyl group to the DNA molecule, often influencing gene expression without altering the underlying DNA sequence. In horses, DNA methylation plays a role in various biological processes, including development, aging, and response to environmental factors. Research on the equine methylome aims to understand its impact on gene regulation, health, and disease susceptibility in horses. This page compiles peer-reviewed research studies and scholarly articles that explore the patterns, functions, and implications of DNA methylation in equine biology and veterinary science.
Methylome and transcriptome data integration reveals aberrantly regulated genes in equine sarcoids. DNA methylation is a key mechanism in transcription regulation, and aberrant methylation is a common and important mechanism in tumor initiation, maintenance, and progression. To find genes that are aberrantly regulated by altered methylation in horse sarcoids, we used reduced representation bisulfite sequencing (RRBS) accompanied by RNA sequencing (RNA-Seq) for methylome (whole genome DNA methylation sequencing) and transcriptome profiling, respectively. We found that the DNA methylation level was generally lower in lesion samples than in controls. In the analyzed samples, a total of 14,692 d...
Differences in the genome, methylome, and transcriptome do not differentiate isolates of Streptococcus equi subsp. equi from horses with acute clinical signs from isolates of inapparent carriers. Streptococcus equi subsp. equi (SEE) is a host-restricted bacterium that causes the common infectious upper respiratory disease known as strangles in horses. Perpetuation of SEE infection appears attributable to inapparent carrier horses because it neither persists long-term in the environment nor infects other host mammals or vectors, and infection results in short-lived immunity. Whether pathogen factors enable SEE to remain in horses without causing clinical signs remains poorly understood. Thus, our objective was to use next-generation sequencing technologies to characterize the genome, me...