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Topic:Sensitivity and Specificity
Sensitivity and specificity are statistical measures used to evaluate the performance of diagnostic tests in horses. Sensitivity refers to the ability of a test to correctly identify horses with a particular disease or condition, while specificity refers to the ability of the test to correctly identify horses that do not have the disease. These metrics are fundamental in assessing the accuracy and reliability of diagnostic tools used in equine medicine. High sensitivity reduces the chance of false negatives, whereas high specificity reduces the chance of false positives. This page compiles peer-reviewed research studies and scholarly articles that explore the application, interpretation, and implications of sensitivity and specificity in equine diagnostics.
[Development of PCR methods for detection of EAV infection]. The goal of this work was the development of suitable (real-time) RT-PCR techniques for fast and sensitive diagnosis of EAV and for molecular-epidemiological characterisation of viral strains, as an alternative to virus isolation. To this purpose two conventional RT-PCR methods and one real-time RT-PCR were adapted to detect the broadest possible spectrum of viral strains. Several dilutions with Bucyrus strain showed a 100-fold higher sensitivity of real-time RT-PCR and heminested RT-PCR compared to simple RT-PCR. Making use of 11 cell culture supernatants of different EAV isolates and 7 semen...
