13-cis-Retinoic acid affect sheath-shaft interaction of equine hair follicles in vitro.
Abstract: A major challenge to the study of hair follicle growth is an appropriate assay system. Because equine mane follicles are large and noncurved, enabling easy dissection; are readily accessible from a single defined source; and possess a long anagen growth phase, we initiated a study of them in culture. As in our previous studies of human and sheep follicles (Dev Biol 165:469, 1994), we found in this system that transection level dictates the pattern of follicle growth in vitro: follicles transected below the sebaceous gland show a type 1 growth pattern (the shaft grows out with an adherent sheath), while nontransected follicles show a type 2 growth pattern (a naked shaft grows out lacking a sheath). In the present study, we searched for compounds that might influence type 1 or type 2 patterns of growth. We found that 13-cis retinoic acid induced, in a concentration-dependent fashion, a type 1-like pattern of growth under conditions for which a type 2 pattern was expected. All-trans-retinoic acid, SR11237 (a synthetic retinoid X receptor-specific ligand), and meta-carboxy-TTNPB (and inactive synthetic retinoid) did not have these properties. We hypothesize that sheath growth¿processing is mediated by the follicle at the level of the sebaceous gland, or by the sebaceous gland itself, and that persistence of the follicle sheath about the outgrowing shaft in vitro (i) in the physical absence of the sebaceous portion of the follicle, or (ii) in the presence of 13-cis-retinoic acid, is due to the reduced expression of a factor that regulates important shaft sheath interactions.
Publication Date: 1996-02-01 PubMed ID: 8601741DOI: 10.1111/1523-1747.ep12343124Google Scholar: Lookup
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- Journal Article
Summary
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The research focuses on studying the influence of 13-cis-retinoic acid on the growth patterns of equine hair follicles in vitro. The study discovered that 13-cis-retinoic acid changes the follicle growth from a “naked” type 2 pattern (shaft growing without a sheath) to a type 1 pattern (shaft growing with a sheath) and hypothesizes that this may be linked to a reduced expression of a key regulatory factor.
Research Methodology
- The researchers used equine mane follicles as the subject of their study because of their size, accessibility, and long anagen growth phase.
- In their study, they noticed a distinct pattern in hair growth based on the transect level: follicles cut below the sebaceous gland presented a type 1 growth pattern (the shaft grows with an adherent sheath), while nontransected follicles manifested a type 2 growth pattern (shaft grows out without a sheath).
- To understand the factors influencing these growth patterns, they experimented with different compounds.
Findings
- The study found that 13-cis retinoic acid induced a type 1-like growth pattern, even under conditions where a type 2 pattern of growth was expected.
- Other compounds such as All-trans-retinoic acid, SR11237 (a synthetic retinoid X receptor-specific ligand), and meta-carboxy-TTNPB (an inactive synthetic retinoid) did not influence the growth patterns in the same way.
Hypothesis and Implications
- The research theorizes that the persistence of a sheath in the outgrowing shaft of follicles in vitro, either in the physical absence of the follicular sebaceous portion or in the presence of 13-cis-retinoic acid, is due to the reduced expression of a factor that regulates crucial shaft-sheath interactions.
- The role of the sebaceous gland in mediating sheath growth and processing is suggested as well.
- These findings contribute to our understanding of the role of 13-cis retinoic acid in hair growth and may open doors to further research on hair growth regulation and potential treatments for related disorders.
Cite This Article
APA
Williams D, Siock P, Stenn K.
(1996).
13-cis-Retinoic acid affect sheath-shaft interaction of equine hair follicles in vitro.
J Invest Dermatol, 106(2), 356-361.
https://doi.org/10.1111/1523-1747.ep12343124 Publication
Researcher Affiliations
- Skin Biology Research Center of Johnson & Johnson, Skillman, NJ 08558-9418, USA.
MeSH Terms
- Animals
- Autoradiography
- Culture Media
- Culture Techniques
- Hair Follicle / anatomy & histology
- Hair Follicle / drug effects
- Hair Follicle / growth & development
- Horses
- Isotretinoin / pharmacology
Citations
This article has been cited 3 times.- Melnik BC. Isotretinoin and FoxO1: A scientific hypothesis. Dermatoendocrinol 2011 Jul;3(3):141-65.
- Sundberg JP, Boggess D, Sundberg BA, Eilertsen K, Parimoo S, Filippi M, Stenn K. Asebia-2J (Scd1(ab2J)): a new allele and a model for scarring alopecia. Am J Pathol 2000 Jun;156(6):2067-75.
- Deacon EM, Pongracz J, Griffiths G, Lord JM. Isoenzymes of protein kinase C: differential involvement in apoptosis and pathogenesis. Mol Pathol 1997 Jun;50(3):124-31.
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