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Home / Equine Research Bank / Vet Comp Orthop Traumatol / 1H NMR investigation of normal and osteo-arthritic synovial ...
Veterinary and comparative orthopaedics and traumatology : V.C.O.T2008; 21(1); 85-88; doi: 10.3415/VCOT-06-12-0101

1H NMR investigation of normal and osteo-arthritic synovial fluid in the horse.

Abstract: Proton magnetic resonance spectroscopy (1H NMR) has been successfully used in the study of many biological fluids. The data presented here report on the metabolic profiles of normal equine synovial fluids compared with osteoarthritic (OA) fluids. Twenty-five OA synovial fluid samples and eight normal ones were collected from the forelimb fetlock joint in 22 horses, aged between five and 24 years. 1H NMR spectroscopy was carried out with a Bruker Avance DRX 500 equiped with a cryo-magnet working at 11 Tesla, and 'Mestre-C 4.9.9.6' software was used to analyze the spectra. The study assessed the increase of lactate, alanine, acetate, N-acetylglucosamine, pyruvate, citrate, creatine/creatinine, glycerol, HDL choline, and a-glucose in OA synovial fluid. The variations observed in samples from horses with OA compared to those in the control group, and similar data found in other studies, confirm that this technique may be useful in the study of joint metabolism. Its practical application may be in the evaluation of the treatment of OA in athletic horses.
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Publication Date: 2008-02-22 PubMed ID: 18288348DOI: 10.3415/VCOT-06-12-0101Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article explores the use of Proton magnetic resonance spectroscopy (1H NMR) to study the metabolic profiles of normal horse synovial fluids in comparison to fluids from horses suffering from Osteoarthritis (OA). The findings from the study confirm that this technique could be useful in studying joint metabolism and evaluating OA treatment in athletic horses.

Introduction of the Research

  • The researchers used proton magnetic resonance spectroscopy (1H NMR), a popular method for studying biological fluids, to examine the metabolic profiles of synovial fluid in horses. Synovial fluid is crucial in lubricating and providing nutrients to the joints.
  • The focus was on comparing the synovial fluid of healthy horses with that from horses suffering from Osteoarthritis (OA), a common joint disorder in horses.

Methodology of the Research

  • The researchers collected 25 OA synovial fluid samples and 8 normal samples from the forelimb fetlock joint of 22 horses, aged between 5 and 24 years.
  • A Bruker Avance DRX 500 equipped with a cryo-magnet was used along with ‘Mestre-C 4.9.9.6’ software for 1H NMR spectroscopy. This setup allowed the researchers to meticulously analyze the metabolic content of the fluids.

Findings of the Research

  • The study identified increased levels of lactate, alanine, acetate, N-acetylglucosamine, pyruvate, citrate, creatine/creatinine, glycerol, HDL choline, and a-glucose in the OA synovial fluid compared to normal joint fluid.
  • This finding, in combination with similar results discovered in other research, confirms the effective application of 1H NMR spectroscopy in examining joint metabolism and the characteristics of OA.

Implications of the Research

  • The metabolic variations found in the synovial fluid of OA horses compared to healthy ones might help in diagnosing OA in horses earlier and more accurately.
  • The practical application of the technique may lay in evaluating the effect of treatments for OA in performance horses, which could potentially lead to improved management and rehabilitation strategies.

Cite This Article

APA
Lacitignola L, Fanizzi FP, Francioso E, Crovace A. (2008). 1H NMR investigation of normal and osteo-arthritic synovial fluid in the horse. Vet Comp Orthop Traumatol, 21(1), 85-88. https://doi.org/10.3415/VCOT-06-12-0101

Publication

Veterinary and comparative orthopaedics and traumatology : V.C.O.T
ISSN: 0932-0814
NlmUniqueID: 8906319
Country: Germany
Language: English
Volume: 21
Issue: 1
Pages: 85-88

Researcher Affiliations

Lacitignola, L
  • Dipartimento di Scienze Cliniche Veterinarie, Facoltà di Medicina Veterinaria, Università degli studi di Teramo, Teramo, Italy. llacitignola@unite.it
Fanizzi, F P
    Francioso, E
      Crovace, A

        MeSH Terms

        • Animals
        • Diagnosis, Differential
        • Horse Diseases / diagnosis
        • Horse Diseases / metabolism
        • Horses / metabolism
        • Magnetic Resonance Spectroscopy / methods
        • Osteoarthritis / diagnosis
        • Osteoarthritis / metabolism
        • Osteoarthritis / veterinary
        • Synovial Fluid / chemistry
        • Synovial Fluid / metabolism

        Citations

        This article has been cited 19 times.
        1. Huang YF, Wang G, Ding L, Bai ZR, Leng Y, Tian JW, Zhang JZ, Li YQ, Ahmad, Qin YH, Li X, Qi X. Lactate-upregulated NADPH-dependent NOX4 expression via HCAR1/PI3K pathway contributes to ROS-induced osteoarthritis chondrocyte damage.. Redox Biol 2023 Nov;67:102867.
          doi: 10.1016/j.redox.2023.102867pubmed: 37688977google scholar: lookup
        2. Laus F, Gialletti R, Bazzano M, Laghi L, Dini F, Marchegiani A. Synovial Fluid Metabolome Can Differentiate between Healthy Joints and Joints Affected by Osteoarthritis in Horses.. Metabolites 2023 Aug 4;13(8).
          doi: 10.3390/metabo13080913pubmed: 37623857google scholar: lookup
        3. Stabile M, Girelli CR, Lacitignola L, Samarelli R, Crovace A, Fanizzi FP, Staffieri F. (1)H-NMR metabolomic profile of healthy and osteoarthritic canine synovial fluid before and after UC-II supplementation.. Sci Rep 2022 Nov 16;12(1):19716.
          doi: 10.1038/s41598-022-23977-1pubmed: 36385297google scholar: lookup
        4. Vignoli A, Risi E, McCartney A, Migliaccio I, Moretti E, Malorni L, Luchinat C, Biganzoli L, Tenori L. Precision Oncology via NMR-Based Metabolomics: A Review on Breast Cancer.. Int J Mol Sci 2021 Apr 28;22(9).
          doi: 10.3390/ijms22094687pubmed: 33925233google scholar: lookup
        5. Nagana Gowda GA, Raftery D. NMR-Based Metabolomics.. Adv Exp Med Biol 2021;1280:19-37.
          doi: 10.1007/978-3-030-51652-9_2pubmed: 33791972google scholar: lookup
        6. Clarke EJ, Anderson JR, Peffers MJ. Nuclear magnetic resonance spectroscopy of biofluids for osteoarthritis.. Br Med Bull 2021 Mar 25;137(1):28-41.
          doi: 10.1093/bmb/ldaa037pubmed: 33290503google scholar: lookup
        7. Tran H, McConville M, Loukopoulos P. Metabolomics in the study of spontaneous animal diseases.. J Vet Diagn Invest 2020 Sep;32(5):635-647.
          doi: 10.1177/1040638720948505pubmed: 32807042google scholar: lookup
        8. Anderson JR, Phelan MM, Foddy L, Clegg PD, Peffers MJ. Ex Vivo Equine Cartilage Explant Osteoarthritis Model: A Metabolomics and Proteomics Study.. J Proteome Res 2020 Sep 4;19(9):3652-3667.
          doi: 10.1021/acs.jproteome.0c00143pubmed: 32701294google scholar: lookup
        9. Akhbari P, Karamchandani U, Jaggard MKJ, Graça G, Bhattacharya R, Lindon JC, Williams HRT, Gupte CM. Can joint fluid metabolic profiling (or "metabonomics") reveal biomarkers for osteoarthritis and inflammatory joint disease?: A systematic review.. Bone Joint Res 2020 Mar;9(3):108-119.
          doi: 10.1302/2046-3758.93.BJR-2019-0167.R1pubmed: 32435463google scholar: lookup
        10. Anderson JR, Phelan MM, Rubio-Martinez LM, Fitzgerald MM, Jones SW, Clegg PD, Peffers MJ. Optimization of Synovial Fluid Collection and Processing for NMR Metabolomics and LC-MS/MS Proteomics.. J Proteome Res 2020 Jul 2;19(7):2585-2597.
          doi: 10.1021/acs.jproteome.0c00035pubmed: 32227958google scholar: lookup
        11. de Sousa EB, Dos Santos Junior GC, Aguiar RP, da Costa Sartore R, de Oliveira ACL, Almeida FCL, Neto VM, Aguiar DP. Osteoarthritic Synovial Fluid Modulates Cell Phenotype and Metabolic Behavior In Vitro.. Stem Cells Int 2019;2019:8169172.
          doi: 10.1155/2019/8169172pubmed: 30766606google scholar: lookup
        12. Martini FM, Brandstetter de Bellesini A, Miolo A, Del Coco L, Fanizzi FP, Crovace A. Combining a joint health supplement with tibial plateau leveling osteotomy in dogs with cranial cruciate ligament rupture. An exploratory controlled trial.. Int J Vet Sci Med 2017 Dec;5(2):105-112.
          doi: 10.1016/j.ijvsm.2017.09.006pubmed: 30255058google scholar: lookup
        13. Anderson JR, Phelan MM, Clegg PD, Peffers MJ, Rubio-Martinez LM. Synovial Fluid Metabolites Differentiate between Septic and Nonseptic Joint Pathologies.. J Proteome Res 2018 Aug 3;17(8):2735-2743.
          doi: 10.1021/acs.jproteome.8b00190pubmed: 29969035google scholar: lookup
        14. Zhai G, Randell EW, Rahman P. Metabolomics of osteoarthritis: emerging novel markers and their potential clinical utility.. Rheumatology (Oxford) 2018 Dec 1;57(12):2087-2095.
          doi: 10.1093/rheumatology/kex497pubmed: 29373736google scholar: lookup
        15. de Sousa EB, Dos Santos GC Junior, Duarte MEL, Moura V Neto, Aguiar DP. Metabolomics as a promising tool for early osteoarthritis diagnosis.. Braz J Med Biol Res 2017 Sep 21;50(11):e6485.
          doi: 10.1590/1414-431X20176485pubmed: 28953990google scholar: lookup
        16. Guma M, Tiziani S, Firestein GS. Metabolomics in rheumatic diseases: desperately seeking biomarkers.. Nat Rev Rheumatol 2016 May;12(5):269-81.
          doi: 10.1038/nrrheum.2016.1pubmed: 26935283google scholar: lookup
        17. Emwas AH, Roy R, McKay RT, Ryan D, Brennan L, Tenori L, Luchinat C, Gao X, Zeri AC, Gowda GA, Raftery D, Steinbeck C, Salek RM, Wishart DS. Recommendations and Standardization of Biomarker Quantification Using NMR-Based Metabolomics with Particular Focus on Urinary Analysis.. J Proteome Res 2016 Feb 5;15(2):360-73.
          doi: 10.1021/acs.jproteome.5b00885pubmed: 26745651google scholar: lookup
        18. Desjardin C, Riviere J, Vaiman A, Morgenthaler C, Diribarne M, Zivy M, Robert C, Le Moyec L, Wimel L, Lepage O, Jacques C, Cribiu E, Schibler L. Omics technologies provide new insights into the molecular physiopathology of equine osteochondrosis.. BMC Genomics 2014 Oct 31;15(1):947.
          doi: 10.1186/1471-2164-15-947pubmed: 25359417google scholar: lookup
        19. Calabrò A, Gralka E, Luchinat C, Saccenti E, Tenori L. A metabolomic perspective on coeliac disease.. Autoimmune Dis 2014;2014:756138.
          doi: 10.1155/2014/756138pubmed: 24665364google scholar: lookup
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