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Genome research1999; 9(12); 1239-1249; doi: 10.1101/gr.9.12.1239

A comparative gene map of the horse (Equus caballus).

Abstract: A comparative gene map of the horse genome composed of 127 loci was assembled based on the new assignment of 68 equine type I loci and on data published previously. PCR primers based on consensus gene sequences conserved across mammalian species were used to amplify markers for assigning 68 equine type I loci to 27 horse synteny groups established previously with a horse-mouse somatic cell hybrid panel (SCHP, UC Davis). This increased the number of coding genes mapped to the horse genome by over 2-fold and allowed refinements of the comparative mapping data available for this species. In conjunction with 57 previous assignments of type I loci to the horse genome map, these data have allowed us to confirm the assignment of 24 equine synteny groups to their respective chromosomes, to provisionally assign nine synteny groups to chromosomes, and to further refine the genetic composition established with Zoo-FISH of two horse chromosomes. The equine type I markers developed in this study provide an important resource for the future development of the horse linkage and physical genome maps.
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Publication Date: 1999-12-30 PubMed ID: 10613847PubMed Central: PMC311003DOI: 10.1101/gr.9.12.1239Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses a comparative gene map of the horse genome, which has been compiled using PCR primers and markers relevant to horse genetics. The compiled data has substantially increased the known coding genes in the horse genome and allowed refinements of the genetic mapping for this species.

Understanding the Horse Genome

  • The research article focuses on understanding the horse (Equus caballus) genome. The researchers compiled a comparative gene map pertaining to 127 locations (loci) within the horse genome. This was achieved by the new assignment of 68 equine type I loci (regions with a notable genetic activity).
  • PCR primers (a short segment of DNA that acts as a starting point for DNA replication) were utilized. These were based on consensus gene sequences conserved across mammalian species. This process allowed for the creation of markers that assisted in assigning 68 equine type I locations.

Role of Horse-Mouse Somatic Cell Hybrid Panel

  • These markers were amplified using the horse-mouse somatic cell hybrid panel (SCHP), a method from UC Davis where cells from two different mammals are merged to create a hybrid cell for genetic research.
  • This procedure increased the number of coding genes (genes involved in the process of transcribing and translating information in DNA into functional proteins) within the horse genome map by more than double.

Refining the Horse Genome

  • This additional data allowed researchers to refine the comparative mapping of the horse genome. The process was also enhanced with 57 prior assignments of type I loci to the horse genome map.
  • They were able to confirm the assignment of 24 equine synteny groups (sets of genes located on the same chromosome) to their respective chromosomes and provisionally assign nine synteny groups.
  • Additionally, they further refined the genetic composition established with Zoo-FISH on two horse chromosomes. Zoo-FISH, or Zoo-FISHUG, is a method of fluorescent in situ hybridization, a technique that visualizes the location of a specific DNA sequence in a chromosome. This technique was used to help refine the map of the horse’s genome.

Future Applications

  • The equine type I markers developed in this study could provide an important resource for future development in mapping the horse genome, both physically and through linkages (the tendency of DNA sequences that are close together on a chromosome to be inherited together).

Cite This Article

APA
Caetano AR, Shiue YL, Lyons LA, O'Brien SJ, Laughlin TF, Bowling AT, Murray JD. (1999). A comparative gene map of the horse (Equus caballus). Genome Res, 9(12), 1239-1249. https://doi.org/10.1101/gr.9.12.1239

Publication

Genome research
ISSN: 1088-9051
NlmUniqueID: 9518021
Country: United States
Language: English
Volume: 9
Issue: 12
Pages: 1239-1249

Researcher Affiliations

Caetano, A R
  • Veterinary Genetics Laboratory, University of California Davis, Davis, California 95616-8744, USA.
Shiue, Y L
    Lyons, L A
      O'Brien, S J
        Laughlin, T F
          Bowling, A T
            Murray, J D

              MeSH Terms

              • Animals
              • Base Sequence / genetics
              • Chromosome Mapping
              • Conserved Sequence
              • DNA Primers / genetics
              • Genetic Markers
              • Genome
              • Horses / genetics
              • Polymerase Chain Reaction
              • Sequence Analysis, DNA
              • Sex Chromosomes / genetics

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              Citations

              This article has been cited 14 times.
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