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Animals : an open access journal from MDPI2021; 11(3); doi: 10.3390/ani11030755

A Comparison of the Colonic Microbiome and Volatile Organic Compound Metabolome of Anoplocephala perfoliata Infected and Non-Infected Horses: A Pilot Study.

Abstract: Anoplocephala perfoliata is a common equine tapeworm associated with an increased risk of colic (abdominal pain) in horses. Identification of parasite and intestinal microbiota interactions have consequences for understanding the mechanisms behind parasite-associated colic and potential new methods for parasite control. A. perfoliata was diagnosed by counting of worms in the caecum post-mortem. Bacterial DNA was extracted from colonic contents and sequenced targeting of the 16S rRNA gene (V4 region). The volatile organic compound (VOC) metabolome of colonic contents was characterised using gas chromatography mass spectrometry. Bacterial diversity (alpha and beta) was similar between tapeworm infected and non-infected controls. Some compositional differences were apparent with down-regulation of operational taxonomic units (OTUs) belonging to the symbiotic families of Ruminococcaceae and Lachnospiraceae in the tapeworm-infected group. Overall tapeworm burden accounted for 7-8% of variation in the VOC profile (permutational multivariate analysis of variance). Integration of bacterial OTUs and VOCs demonstrated moderate to strong correlations indicating the potential of VOCs as markers for bacterial OTUs in equine colonic contents. This study has shown potential differences in the intestinal microbiome and metabolome of A. perfoliata infected and non-infected horses. This pilot study did not control for extrinsic factors including diet, disease history and stage of infection.
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Publication Date: 2021-03-09 PubMed ID: 33803473PubMed Central: PMC7999024DOI: 10.3390/ani11030755Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study aims to compare the colonic microbiome and metabolome of horses infected with the common equine tapeworm, Anoplocephala perfoliata, with those of non-infected horses for better understanding of parasite-associated colic and possible measures for controlling the parasite.

Methodology

  • Diagnosis of Anoplocephala perfoliata infection was done by counting the number of worms in the horse’s caecum post-mortem.
  • Bacterial DNA was extracted from the colonic contents and sequenced, specifically targeting the V4 region of the 16S rRNA gene.
  • The volatile organic compound (VOC) metabolome of the colonic contents was characterised using gas chromatography mass spectrometry.

Results

  • There were similar bacterial diversities for both the tapeworm-infected horses and the non-infected ones. However, there were some compositional differences, with the symbiotic families of Ruminococcaceae and Lachnospiraceae being down-regulated in the infected group.
  • Tapeworm burden accounted for around 7-8% of the variation in the VOC profile. The integration of bacterial OTUs (operational taxonomic units) and VOCs displayed moderate to strong correlations, suggesting the potential of VOCs as markers for bacterial OTUs in equine colonic contents.

Conclusion

  • The research showed potential differences in the intestinal microbiome and metabolome between Anoplocephala perfoliata infected and non-infected horses. However, the pilot study did not control for external factors like diet, disease history and stage of infection, which may have had an influence on the results.
  • Understanding the interactions between parasite and intestinal microbiota can aid in understanding mechanisms behind parasite-associated colic in horses, and potentially give rise to new methods for parasite control.

Cite This Article

APA
Slater R, Frau A, Hodgkinson J, Archer D, Probert C. (2021). A Comparison of the Colonic Microbiome and Volatile Organic Compound Metabolome of Anoplocephala perfoliata Infected and Non-Infected Horses: A Pilot Study. Animals (Basel), 11(3). https://doi.org/10.3390/ani11030755

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 11
Issue: 3

Researcher Affiliations

Slater, Rachael
  • Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Crown Street, Liverpool L69 3GE, UK.
Frau, Alessandra
  • Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Crown Street, Liverpool L69 3GE, UK.
Hodgkinson, Jane
  • Institute of Infection, Veterinary and Ecological Science, University of Liverpool, Leahurst Campus, Chester High Road, Wirral CH64 7TE, UK.
Archer, Debra
  • Institute of Infection, Veterinary and Ecological Science, University of Liverpool, Leahurst Campus, Chester High Road, Wirral CH64 7TE, UK.
Probert, Chris
  • Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Crown Street, Liverpool L69 3GE, UK.

Conflict of Interest Statement

The authors declare no conflict of interest.

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