A computer-assisted microscopic analysis of bone tissue developed inside a polyactive polymer implanted into an equine articular surface.
Abstract: One of the most promising applications for the restoration of small or moderately sized focal articular lesions is mosaicplasty (MP). Although recurrent hemarthrosis is a rare complication after MP, recently, various strategies have been designed to find an effective filling material to prevent postoperative bleeding from the donor site. The porous biodegradable polymer Polyactive (PA; a polyethylene glycol terephthalate - polybutylene terephthalate copolymer) represents a promising solution in this respect. A histological evaluation of the longterm PA-filled donor sites obtained from 10 experimental horses was performed. In this study, attention was primarily focused on the bone tissue developed in the plug. A computer-assisted image analysis and quantitative polarized light microscopic measurements of decalcified, longitudinally sectioned, dimethylmethylene blue (DMMB)- and picrosirius red (PS) stained sections revealed that the coverage area of the bone trabecules in the PA-filled donor tunnels was substantially (25%) enlarged compared to the neighboring cancellous bone. For this quantification, identical ROIs (regions of interest) were used and compared. The birefringence retardation values were also measured with a polarized light microscope using monochromatic light. Identical retardation values could be recorded from the bone trabeculae developed in the PA and in the neighboring bone, which indicates that the collagen orientation pattern does not differ significantly among these bone trabecules. Based on our new data, we speculate that PA promotes bone formation, and some of the currently identified degradation products of PA may enhance osteo-conduction and osteoinduction inside the donor canal.
Publication Date: 2012-07-19 PubMed ID: 22806907DOI: 10.14670/HH-27.1203Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research article explores the use of the biodegradable polymer, Polyactive, in mosaicplasty operations, a method used to repair smaller articular lesions. The authors executed a detailed analysis of bone tissue developed in Polyactive-filled donor sites in horses, focusing on the tissue within the polymer plug. The results indicated an increase in bone trabecules coverage area and suggested that Polyactive aids bone formation.
Introduction
- Mosaicplasty (MP) is a microsurgical procedure employed primarily to treat small or moderate articular lesions in joints. One of the challenges is the prevention of postoperative bleeding, a rare yet significant complication after such procedures.
- Researchers are continually seeking effective filling materials for donor sites to avert this issue. Polyactive (PA), a porous biodegradable polymer, has gained prominence in this regard due to its potential in successfully addressing this problem.
Methodology
- The researchers performed a histological evaluation on the Polyactive-filled donor sites in experimental horses, with a specific focus on the bone tissue that developed within the plug.
- A computer-assisted image analysis was conducted on the decalcified, longitudinally sectioned samples that were stained with DMMB and PS. This was coupled with quantitative polarized light microscopic measurements.
- Through identical regions of interest (ROIs), the researchers assessed and compared the coverage area of the bone trabecules in different parts of the horse’s joint.
Results
- The team discovered an increased (25%) coverage area of bone trabecules in Polyactive-filled donor sites compared to the adjacent cancellous bone.
- Using a polarized light microscope, they documented identical birefringence retardation values in both the new bone trabecules in Polyactive and the neighboring bone. This suggests the orientation pattern of collagen, a primary component of bone, remains consistent between both types of bone trabecules.
Conclusion
- Given the results, the authors suggested that Polyactive likely promotes bone formation. They also speculated that some of the found degradation products of Polyactive could enhance osteo-conduction and osteo-induction within the donor canal.
Cite This Article
APA
Albert R, Vásárhelyi G, Bodó G, Kenyeres A, Wolf E, Papp T, Terdik T, Módis L, Felszeghy S.
(2012).
A computer-assisted microscopic analysis of bone tissue developed inside a polyactive polymer implanted into an equine articular surface.
Histol Histopathol, 27(9), 1203-1209.
https://doi.org/10.14670/HH-27.1203 Publication
Researcher Affiliations
- Department of Anatomy, Histology and Embryology, Medical and Health Science Centre, University of Debrecen, Debrecen, Hungary.
MeSH Terms
- Animals
- Arthroplasty / methods
- Biocompatible Materials
- Bone Transplantation / methods
- Cartilage / transplantation
- Horses
- Image Processing, Computer-Assisted
- Knee Joint / surgery
- Osseointegration / drug effects
- Osteogenesis
- Polyesters / therapeutic use
- Polyethylene Glycols / therapeutic use
Citations
This article has been cited 2 times.- Carvalho JRG, Conde G, Antonioli ML, Santana CH, Littiere TO, Dias PP, Chinelatto MA, Canola PA, Zara FJ, Ferraz GC. Long-Term Evaluation of Poly(lactic acid) (PLA) Implants in a Horse: An Experimental Pilot Study. Molecules 2021 Nov 29;26(23).
- Hanssen NM, Schotanus MG, Verburg AD. Osteolysis in cemented total hip arthroplasty involving the OptiPlug cement restrictor: more than an incident?. Eur J Orthop Surg Traumatol 2015 Jan;25(1):45-51.
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