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A genome-wide landscape of mRNAs, miRNAs, lncRNAs, and circRNAs of skeletal muscles during dietary restriction in Mongolian horses.
Abstract: The proportion of different muscle fibers is essential for the horse breed's aptitude for athletic activities. Adaptation of locomotor muscle is correlated with altered physiologic conditions. To investigate the adaptive changes of muscle fiber phenotype and transcriptome in horse skeletal muscle during dietary restriction (DR). The muscle fiber type distribution and deep RNA-seq analysis of detecting differentially expressed mRNAs (DEGs), miRNA (DEMIRs), lncRNAs (DELs), circRNAs (DECs), and their function analysis were investigated in gluteus medius muscle of Mongolian horses during DR. A total of 1433 DEGs, 5 DEMIRs, 329 DELs, and 53 DECs were identified. Differing from non-uniform muscle fiber type changing, functional enrichment analysis showed that most downregulated DEGs were associated in muscle contraction, fuel energy metabolism, and protein balance. Linkages between non-coding RNA and mRNA landscape were detected from their functional changes. Our study provides new insights into the expressional changes of mRNA and non-coding RNA in horse skeletal muscles during DR, which might improve our understanding of the molecular mechanisms regulating muscle adaption during DR for racing horses.
Copyright © 2023 Elsevier Inc. All rights reserved.
Publication Date: 2023-05-02 PubMed ID: 37150091DOI: 10.1016/j.cbd.2023.101084Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research examined how changes in diet affect the genetic expression in the skeletal muscles of Mongolian horses. Scientists studied different types of RNA in muscle fibres to understand how muscle function adapts during dietary restriction.
Overview of Research Conducted
The researchers focused on the Mongolian horse skeletal muscles’ genetic landscape under dietary restriction. They used RNA-sequence analysis to examine the changes in different RNA types including:
- mRNAs or messenger RNAs which carry genetic information from the DNA to the ribosome for protein synthesis,
- miRNAs or microRNAs, small non-coding RNAs that regulate gene expression,
- lncRNAs or long non-coding RNAs, which also regulate gene function and expression, and
- circRNAs or circular RNAs, another type of non-coding RNA, thought to be involved in gene regulation.
Findings
A total of 1,433 differentially expressed genes (DEGs), 5 differentially expressed miRNAs (DEMIRs), 329 differentially expressed lncRNAs (DELs), and 53 differentially expressed circRNAs (DECs) were identified. These findings suggest significant genomic changes occur in the skeletal muscle of horses under dietary restriction.
The researchers also observed that the changes in muscle fiber type were not uniform. Analysis of the DEGs showed that most downregulated or less active genes were associated with muscle contraction, fuel energy metabolism, and protein balance, implying dietary restriction has significant functional implications for muscle operation.
The Importance of Non-Coding RNA in Muscle Adaptation
The study highlighted the role of non-coding RNAs in the regulation of gene function during dietary restriction. It linked the function changes seen in non-coding RNA and mRNA, pointing to a deeper understanding of how RNA mechanisms regulate muscle adaptation under restricted dietary conditions.
Implications and Conclusions
This detailed exploration of the role and changes in different types of RNA during dietary restriction offers new perspectives on how horse skeletal muscles adjust at the molecular level. The insights generated could potentially help improve the understanding of the mechanisms governing muscle adaptation during dietary restriction in racehorses. This understanding could lead to better diet and training regimens for horses to maximize their athletic abilities.
Cite This Article
APA
Bou T, Ding W, Liu H, Gong W, Jia Z, Dugarjaviin M, Bai D.
(2023).
A genome-wide landscape of mRNAs, miRNAs, lncRNAs, and circRNAs of skeletal muscles during dietary restriction in Mongolian horses.
Comp Biochem Physiol Part D Genomics Proteomics, 46, 101084.
https://doi.org/10.1016/j.cbd.2023.101084 Publication
Researcher Affiliations
- Key Laboratory of Equus Germplasm Innovation (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs; Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction; Equus Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China. Electronic address: https://twitter.com/@BTvgqin.
- Key Laboratory of Equus Germplasm Innovation (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs; Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction; Equus Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
- Key Laboratory of Equus Germplasm Innovation (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs; Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction; Equus Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
- Key Laboratory of Equus Germplasm Innovation (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs; Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction; Equus Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
- Key Laboratory of Equus Germplasm Innovation (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs; Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction; Equus Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
- Key Laboratory of Equus Germplasm Innovation (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs; Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction; Equus Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
- Key Laboratory of Equus Germplasm Innovation (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs; Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction; Equus Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China. Electronic address: baidongyi1983@163.com.
MeSH Terms
- Horses / genetics
- Animals
- RNA, Long Noncoding / genetics
- MicroRNAs / genetics
- RNA, Circular / genetics
- RNA, Messenger / genetics
- Gene Expression Profiling
- Muscle, Skeletal / metabolism
Conflict of Interest Statement
Declaration of competing interest The authors declared that they have no conflicts of interest to this work. We declare that we do not have any commercial or associative interest that represents a conflict of interest in connection with the work submitted.
Citations
This article has been cited 4 times.- Wang T, Li M, Ren W, Meng J, Yao X, Chu H, Yao R, Zhai M, Zeng Y. Multi-Omics Analysis Reveals Biaxial Regulatory Mechanisms of Cardiac Adaptation by Specialized Racing Training in Yili Horses. Biology (Basel) 2025 Nov 17;14(11).
- Baatar T, Song D, Weng Y, Wang G, Jin L, Guo R, Li B, Dugarjaviin M. Effect of Alternative Splicing Euchromatic Histone Lysine Methyltransferase 2 (EHMT2/G9A) on Spermatogenesis in Mongolian Horses. Animals (Basel) 2025 Apr 11;15(8).
- Xu L, Jin T, Lou A, Guan J, Zhang X, Wang H, Guan L. The effect of miR-23b-3p on regulating GH by targeting POU1F1 in Yanbian yellow cattle. Anim Biotechnol 2024 Nov;35(1):2346808.
- Liu Y, Du M, Li X, Zhang L, Zhao B, Wang N, Dugarjaviin M. Single-Cell Transcriptome Sequencing Reveals Molecular Expression Differences and Marker Genes in Testes during the Sexual Maturation of Mongolian Horses. Animals (Basel) 2024 Apr 23;14(9).
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