FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / ScientificWorldJournal / A multigene approach for comparing genealogy of Betacoronavi...
TheScientificWorldJournal2013; 2013; 349702; doi: 10.1155/2013/349702

A multigene approach for comparing genealogy of Betacoronavirus from cattle and horses.

Abstract: Gastroenteritis is one of the leading causes of morbidity and mortality among young and newborn animals and is often caused by multiple intestinal infections, with rotavirus and bovine coronavirus (BCoV) being the main viral causes in cattle. Given that BCoV is better studied than equine coronaviruses and given the possibility of interspecies transmission of these viruses, this research was designed to compare the partial sequences of the spike glycoprotein (S), hemagglutinin-esterase protein (HE), and nucleoprotein (N) genes from coronaviruses from adult cattle with winter dysentery, calves with neonatal diarrhea, and horses. To achieve this, eleven fecal samples from dairy cows with winter dysentery, three from calves, and two from horses, all from Brazil, were analysed. It could be concluded that the enteric BCoV genealogy from newborn and adult cattle is directly associated with geographic distribution patterns, when S and HE genes are taken into account. A less-resolved genealogy exists for the HE and N genes in cattle, with a trend for an age-related segregation pattern. The coronavirus strains from horses revealed Betacoronavirus sequences indistinguishable from those found in cattle, a fact previously unknown.
Get Full Text
Publication Date: 2013-11-17 PubMed ID: 24348152PubMed Central: PMC3855977DOI: 10.1155/2013/349702Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article analysed the gene sequences of coronaviruses found in cattle and horses. The study suggests that transmission of these viruses may occur between the two species, indicating a need for further understanding and prevention measures.

Research Objective and Method

  • The primary aim of this research was to study and compare the gene sequences of coronaviruses prevalent in cattle and horses. This objective was driven by the necessity to understand potential interspecies transmission—a phenomenon where a pathogen jumps from one species to another.
  • The researchers analyzed three partial gene sequences—spike glycoprotein (S), hemagglutinin-esterase protein (HE), and nucleoprotein (N)—in coronaviruses from adult cattle with winter dysentery, calves with neonatal diarrhea, and horses. These samples were all sourced from Brazil.
  • The team utilized a multi gene approach to present a comprehensive view of the virus’s behavior and evolution in both species.

Key Findings

  • Through this multi gene analysis, the researchers found that the coronaviruses’ genealogy or gene sequences in newborn and adult cattle were strongly linked to geographical distribution patterns. This was especially true when the S and HE genes were considered.
  • However, a more convoluted genealogy was discovered for the HE and N genes in cattle. Rather than a strict adherence to geographical distribution, these genes seemed to display an age-related segregation pattern.
  • What was particularly striking about the research was the discovery that the strains of coronaviruses found in horses revealed gene sequences, specifically Betacoronaviruses, which were nearly indistinguishable from those found in cattle.

Implications and Conclusions

  • This discovery presented a previously unknown aspect of coronavirus genealogy—that they may be transmitted interspecies, particularly between cattle and horses.
  • This finding suggests a need to study further the mechanisms of interspecies transmission and develop ways of monitoring and preventing this from happening. It could have significant implications for viral disease resilience in livestock populations.

Cite This Article

APA
Barros IN, Silva SO, Nogueira Neto FS, Asano KM, Souza SP, Richtzenhain LJ, Brandao PE. (2013). A multigene approach for comparing genealogy of Betacoronavirus from cattle and horses. ScientificWorldJournal, 2013, 349702. https://doi.org/10.1155/2013/349702

Publication

TheScientificWorldJournal
ISSN: 1537-744X
NlmUniqueID: 101131163
Country: United States
Language: English
Volume: 2013
Pages: 349702
PII: 349702

Researcher Affiliations

Barros, Iracema N
  • Department of Preventive Veterinary Medicine and Animal Health, College of Veterinary Medicine, University of São Paulo, Avenue Professor Dr. Orlando Marques de Paiva 87, Cidade Universitária, 05508-270 São Paulo, SP, Brazil ; Coronavirus Research Group, College of Veterinary Medicine, University of São Paulo, Avenue Professor Dr. Orlando Marques de Paiva 87, Cidade Universitária, 05508-270 São Paulo, SP, Brazil.
Silva, Sheila O S
  • Department of Preventive Veterinary Medicine and Animal Health, College of Veterinary Medicine, University of São Paulo, Avenue Professor Dr. Orlando Marques de Paiva 87, Cidade Universitária, 05508-270 São Paulo, SP, Brazil ; Coronavirus Research Group, College of Veterinary Medicine, University of São Paulo, Avenue Professor Dr. Orlando Marques de Paiva 87, Cidade Universitária, 05508-270 São Paulo, SP, Brazil.
Nogueira Neto, Francisco S
  • Jockey Club of São Paulo, Bento Frias Street 248, Group 555, 05423-050 São Paulo, SP, Brazil.
Asano, Karen M
  • Department of Preventive Veterinary Medicine and Animal Health, College of Veterinary Medicine, University of São Paulo, Avenue Professor Dr. Orlando Marques de Paiva 87, Cidade Universitária, 05508-270 São Paulo, SP, Brazil ; Coronavirus Research Group, College of Veterinary Medicine, University of São Paulo, Avenue Professor Dr. Orlando Marques de Paiva 87, Cidade Universitária, 05508-270 São Paulo, SP, Brazil.
Souza, Sibele P
  • Department of Preventive Veterinary Medicine and Animal Health, College of Veterinary Medicine, University of São Paulo, Avenue Professor Dr. Orlando Marques de Paiva 87, Cidade Universitária, 05508-270 São Paulo, SP, Brazil ; Coronavirus Research Group, College of Veterinary Medicine, University of São Paulo, Avenue Professor Dr. Orlando Marques de Paiva 87, Cidade Universitária, 05508-270 São Paulo, SP, Brazil.
Richtzenhain, Leonardo J
  • Department of Preventive Veterinary Medicine and Animal Health, College of Veterinary Medicine, University of São Paulo, Avenue Professor Dr. Orlando Marques de Paiva 87, Cidade Universitária, 05508-270 São Paulo, SP, Brazil ; Coronavirus Research Group, College of Veterinary Medicine, University of São Paulo, Avenue Professor Dr. Orlando Marques de Paiva 87, Cidade Universitária, 05508-270 São Paulo, SP, Brazil.
Brandao, Paulo E
  • Department of Preventive Veterinary Medicine and Animal Health, College of Veterinary Medicine, University of São Paulo, Avenue Professor Dr. Orlando Marques de Paiva 87, Cidade Universitária, 05508-270 São Paulo, SP, Brazil ; Coronavirus Research Group, College of Veterinary Medicine, University of São Paulo, Avenue Professor Dr. Orlando Marques de Paiva 87, Cidade Universitária, 05508-270 São Paulo, SP, Brazil.

MeSH Terms

  • Animals
  • Brazil
  • Cattle
  • Coronavirus, Bovine / classification
  • Coronavirus, Bovine / genetics
  • Genes, Viral
  • Geography
  • Horses
  • Phylogeny

References

This article includes 33 references
  1. De Groot RJ, Ziebuhr J, Poon LL. Taxonomic Proposal to the ICTV Executive Committee: revision of the family Coronaviridaen. ICTV 2008.
  2. . Virus Taxonomy: 2009 release. ICTV-International Committee on Taxonomy of Viruses 2009.
  3. Lai MMC, Holmes KV. Coronaviridae: the viruses and their replication. Fields Virology 4th edition. Philadelphia, Pa, USA: Lippincott Williams & Wilkins; 2001. pp. 1163–1185.
  4. Masters PS. The molecular biology of coronaviruses.. Adv Virus Res 2006;66:193-292.
    pmc: PMC7112330pubmed: 16877062doi: 10.1016/s0065-3527(06)66005-3google scholar: lookup
  5. Tegtmeier C, Uttenthal A, Friis NF, Jensen NE, Jensen HE. Pathological and microbiological studies on pneumonic lungs from Danish calves.. Zentralbl Veterinarmed B 1999 Dec;46(10):693-700.
    pmc: PMC7183811pubmed: 10676147doi: 10.1046/j.1439-0450.1999.00301.xgoogle scholar: lookup
  6. Boileau MJ, Kapil S. Bovine coronavirus associated syndromes.. Vet Clin North Am Food Anim Pract 2010 Mar;26(1):123-46, table of contents.
    pmc: PMC7125561pubmed: 20117547doi: 10.1016/j.cvfa.2009.10.003google scholar: lookup
  7. Guy JS, Breslin JJ, Breuhaus B, Vivrette S, Smith LG. Characterization of a coronavirus isolated from a diarrheic foal.. J Clin Microbiol 2000 Dec;38(12):4523-6.
    pmc: PMC87631pubmed: 11101590doi: 10.1128/jcm.38.12.4523-4526.2000google scholar: lookup
  8. Davis E, Rush BR, Cox J, DeBey B, Kapil S. Neonatal enterocolitis associated with coronavirus infection in a foal: a case report.. J Vet Diagn Invest 2000 Mar;12(2):153-6.
    pubmed: 10730946doi: 10.1177/104063870001200210google scholar: lookup
  9. Zhang J, Guy JS, Snijder EJ, Denniston DA, Timoney PJ, Balasuriya UB. Genomic characterization of equine coronavirus.. Virology 2007 Dec 5;369(1):92-104.
    pmc: PMC7103287pubmed: 17706262doi: 10.1016/j.virol.2007.06.035google scholar: lookup
  10. Oue Y, Ishihara R, Edamatsu H, Morita Y, Yoshida M, Yoshima M, Hatama S, Murakami K, Kanno T. Isolation of an equine coronavirus from adult horses with pyrogenic and enteric disease and its antigenic and genomic characterization in comparison with the NC99 strain.. Vet Microbiol 2011 May 12;150(1-2):41-8.
    pmc: PMC7117184pubmed: 21273011doi: 10.1016/j.vetmic.2011.01.004google scholar: lookup
  11. Akashi H, Inaba Y, Miura Y. Properties of a coronavirus isolated from a cow with epizootic diarrhea. Veterinary Microbiology 1980;5(4):265–276.
  12. Brandão PE, Gregori F, Richtzenhain LJ, Rosales CA, Villarreal LY, Jerez JA. Molecular analysis of Brazilian strains of bovine coronavirus (BCoV) reveals a deletion within the hypervariable region of the S1 subunit of the spike glycoprotein also found in human coronavirus OC43.. Arch Virol 2006 Sep;151(9):1735-48.
    pmc: PMC7086848pubmed: 16583154doi: 10.1007/s00705-006-0752-9google scholar: lookup
  13. Souza SP, Asano KM, Buitrago LYV, Silva SOS, Richtzenhain LJ, Brandão PE. A semi-nested RT-PCR assay targeted to hemagglutinin-esterase gene of Bovine Coronavirus. Brazilian Journal of Veterinary Research and Animal Science 2010;47:323–328.
  14. Asano KM, de Souza SP, de Barros IN, Ayres GR, Silva SO, Richtzenhain LJ, Brandão PE. Multiplex semi-nested RT-PCR with exogenous internal control for simultaneous detection of bovine coronavirus and group A rotavirus.. J Virol Methods 2010 Nov;169(2):375-9.
    pmc: PMC7119652pubmed: 20723564doi: 10.1016/j.jviromet.2010.08.008google scholar: lookup
  15. Hall TA. Bioedit 7.0.9.0: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 1999;41:95–98.
  16. Tamura K, Dudley J, Nei M, Kumar S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0.. Mol Biol Evol 2007 Aug;24(8):1596-9.
    pubmed: 17488738doi: 10.1093/molbev/msm092google scholar: lookup
  17. Decaro N, Martella V, Elia G, Campolo M, Mari V, Desario C, Lucente MS, Lorusso A, Greco G, Corrente M, Tempesta M, Buonavoglia C. Biological and genetic analysis of a bovine-like coronavirus isolated from water buffalo (Bubalus bubalis) calves.. Virology 2008 Jan 5;370(1):213-22.
    pmc: PMC7103353pubmed: 17916374doi: 10.1016/j.virol.2007.08.031google scholar: lookup
  18. Decaro N, Cirone F, Mari V, Nava D, Tinelli A, Elia G, Di Sarno A, Martella V, Colaianni ML, Aprea G, Tempesta M, Buonavoglia C. Characterisation of bubaline coronavirus strains associated with gastroenteritis in water buffalo (Bubalus bubalis) calves.. Vet Microbiol 2010 Oct 26;145(3-4):245-51.
    pmc: PMC7117158pubmed: 20483547doi: 10.1016/j.vetmic.2010.04.010google scholar: lookup
  19. Cebra CK, Mattson DE, Baker RJ, Sonn RJ, Dearing PL. Potential pathogens in feces from unweaned llamas and alpacas with diarrhea.. J Am Vet Med Assoc 2003 Dec 15;223(12):1806-8.
    pubmed: 14690211doi: 10.2460/javma.2003.223.1806google scholar: lookup
  20. Genova SG, Streeter RN, Simpson KM, Kapil S. Detection of an antigenic group 2 coronavirus in an adult alpaca with enteritis.. Clin Vaccine Immunol 2008 Oct;15(10):1629-32.
    pmc: PMC2565933pubmed: 18716008doi: 10.1128/cvi.00232-08google scholar: lookup
  21. Yokoi K, Okazaki H, Inahara K, Hatama S. Prevalence of eight bovine viruses in sika deer (Cervus nippon yesoensis) in Japan.. Vet Rec 2009 Dec 26;165(25):754-5.
    pubmed: 20023282
  22. Hasoksuz M, Alekseev K, Vlasova A, Zhang X, Spiro D, Halpin R, Wang S, Ghedin E, Saif LJ. Biologic, antigenic, and full-length genomic characterization of a bovine-like coronavirus isolated from a giraffe.. J Virol 2007 May;81(10):4981-90.
    pmc: PMC1900194pubmed: 17344285doi: 10.1128/jvi.02361-06google scholar: lookup
  23. Saif LJ. Bovine respiratory coronavirus.. Vet Clin North Am Food Anim Pract 2010 Jul;26(2):349-64.
    pmc: PMC4094360pubmed: 20619189doi: 10.1016/j.cvfa.2010.04.005google scholar: lookup
  24. Cho KO, Hasoksuz M, Nielsen PR, Chang KO, Lathrop S, Saif LJ. Cross-protection studies between respiratory and calf diarrhea and winter dysentery coronavirus strains in calves and RT-PCR and nested PCR for their detection.. Arch Virol 2001 Dec;146(12):2401-19.
    pmc: PMC7087283pubmed: 11811688doi: 10.1007/s007050170011google scholar: lookup
  25. Tråvén M, Näslund K, Linde N, Linde B, Silván A, Fossum C, Hedlund KO, Larsson B. Experimental reproduction of winter dysentery in lactating cows using BCV -- comparison with BCV infection in milk-fed calves.. Vet Microbiol 2001 Jul 26;81(2):127-51.
    pmc: PMC7117383pubmed: 11376958doi: 10.1016/s0378-1135(01)00337-6google scholar: lookup
  26. Park SJ, Jeong C, Yoon SS, Choy HE, Saif LJ, Park SH, Kim YJ, Jeong JH, Park SI, Kim HH, Lee BJ, Cho HS, Kim SK, Kang MI, Cho KO. Detection and characterization of bovine coronaviruses in fecal specimens of adult cattle with diarrhea during the warmer seasons.. J Clin Microbiol 2006 Sep;44(9):3178-88.
    pmc: PMC1594715pubmed: 16954245doi: 10.1128/jcm.02667-05google scholar: lookup
  27. Park SJ, Kim GY, Choy HE, Hong YJ, Saif LJ, Jeong JH, Park SI, Kim HH, Kim SK, Shin SS, Kang MI, Cho KO. Dual enteric and respiratory tropisms of winter dysentery bovine coronavirus in calves.. Arch Virol 2007;152(10):1885-900.
    pmc: PMC7087358pubmed: 17564760doi: 10.1007/s00705-007-1005-2google scholar: lookup
  28. Decaro N, Mari V, Desario C, Campolo M, Elia G, Martella V, Greco G, Cirone F, Colaianni ML, Cordioli P, Buonavoglia C. Severe outbreak of bovine coronavirus infection in dairy cattle during the warmer season.. Vet Microbiol 2008 Jan 1;126(1-3):30-9.
    pmc: PMC7117129pubmed: 17669602doi: 10.1016/j.vetmic.2007.06.024google scholar: lookup
  29. Ball LA. Virus replication strategies. Fields’ Virology 5th edition. Philadelphia, Pa, USA: Lippincott Williams & Wilkins; 2007. pp. 119–140.
  30. Lai MM, Cavanagh D. The molecular biology of coronaviruses.. Adv Virus Res 1997;48:1-100.
    pmc: PMC7130985pubmed: 9233431doi: 10.1016/s0065-3527(08)60286-9google scholar: lookup
  31. Drosten C, Günther S, Preiser W, van der Werf S, Brodt HR, Becker S, Rabenau H, Panning M, Kolesnikova L, Fouchier RA, Berger A, Burguière AM, Cinatl J, Eickmann M, Escriou N, Grywna K, Kramme S, Manuguerra JC, Müller S, Rickerts V, Stürmer M, Vieth S, Klenk HD, Osterhaus AD, Schmitz H, Doerr HW. Identification of a novel coronavirus in patients with severe acute respiratory syndrome.. N Engl J Med 2003 May 15;348(20):1967-76.
    pubmed: 12690091doi: 10.1056/nejmoa030747google scholar: lookup
  32. Marra MA, Jones SJ, Astell CR, Holt RA, Brooks-Wilson A, Butterfield YS, Khattra J, Asano JK, Barber SA, Chan SY, Cloutier A, Coughlin SM, Freeman D, Girn N, Griffith OL, Leach SR, Mayo M, McDonald H, Montgomery SB, Pandoh PK, Petrescu AS, Robertson AG, Schein JE, Siddiqui A, Smailus DE, Stott JM, Yang GS, Plummer F, Andonov A, Artsob H, Bastien N, Bernard K, Booth TF, Bowness D, Czub M, Drebot M, Fernando L, Flick R, Garbutt M, Gray M, Grolla A, Jones S, Feldmann H, Meyers A, Kabani A, Li Y, Normand S, Stroher U, Tipples GA, Tyler S, Vogrig R, Ward D, Watson B, Brunham RC, Krajden M, Petric M, Skowronski DM, Upton C, Roper RL. The Genome sequence of the SARS-associated coronavirus.. Science 2003 May 30;300(5624):1399-404.
    pubmed: 12730501doi: 10.1126/science.1085953google scholar: lookup
  33. Gorbalenya AE, Snijder EJ, Spaan WJ. Severe acute respiratory syndrome coronavirus phylogeny: toward consensus.. J Virol 2004 Aug;78(15):7863-6.
    pmc: PMC446116pubmed: 15254158doi: 10.1128/jvi.78.15.7863-7866.2004google scholar: lookup

Citations

This article has been cited 3 times.
  1. Jevšnik Virant M, Černe D, Petrovec M, Paller T, Toplak I. Genetic Characterisation and Comparison of Three Human Coronaviruses (HKU1, OC43, 229E) from Patients and Bovine Coronavirus (BCoV) from Cattle with Respiratory Disease in Slovenia.. Viruses 2021 Apr 15;13(4).
    doi: 10.3390/v13040676pubmed: 33920821google scholar: lookup
  2. de Mira Fernandes A, Brandão PE, Dos Santos Lima M, de Souza Nunes Martins M, da Silva TG, da Silva Cardoso Pinto V, de Paula LT, Vicente MES, Okuda LH, Pituco EM. Genetic diversity of BCoV in Brazilian cattle herds.. Vet Med Sci 2018 Apr 24;4(3):183-9.
    doi: 10.1002/vms3.102pubmed: 29687958google scholar: lookup
  3. Bok M, Miño S, Rodriguez D, Badaracco A, Nuñes I, Souza SP, Bilbao G, Louge Uriarte E, Galarza R, Vega C, Odeon A, Saif LJ, Parreño V. Molecular and antigenic characterization of bovine Coronavirus circulating in Argentinean cattle during 1994-2010.. Vet Microbiol 2015 Dec 31;181(3-4):221-9.
    doi: 10.1016/j.vetmic.2015.10.017pubmed: 26520931google scholar: lookup
Subscribe to our newsletter
Join 99,344 horse owners like you who receive our email updates on horse health and nutrition.