A new collagen biomatrix of equine origin versus a cadaveric dura graft for the repair of dural defects–a comparative animal experimental study.
Abstract: Numerous types of materials have been evaluated over the past decades in the quest for the ideal dural replacement, but no product fully meets all the applicable criteria. This paper presents the long-term results of an animal trial of a collagen biomatrix (TISSUDURA, Baxter AG, Vienna/Austria) for the repair and regeneration of dural defects. This product provides a matrix with a special layer structure and consists of pure naturally cross-linked collagen of equine origin. The comparable material is Tutoplast Dura, a human cadaveric-derived dural graft preserved in a multiple stage chemical process. Methods: Bihemispheric parietotoccipital dural defects of a defined size were induced in 25 sheep and subsequently closed with the collagen graft or Tutoplast Dura, respectively. Results: Postoperative neurological abnormalities did not occur in any animal. There were no signs of graft rejection or formation of cerebrospinal fluid fistulae. The collagen graft site displayed histological signs as early as 2 weeks postoperatively consistent with a moderate Iymphocytic inflammatory response and infiltration with fibroblasts and macrophages. The graft subsequently developed a neodura-like connective tissue architecture. The Tutoplast Dura showed only minimal cellular infiltration, and again the surrounding structures displayed a mild inflammatory response. 24 weeks postoperatively, the dura and neomembrane were barely distinguishable at the collagen biomatrix site, but the Tutoplast Dura displayed inadequate fusion with the dural border and was encapsulated in a connective tissue layer. Conclusions: The outcomes demonstrate that the collagen graft can be used for dura reconstruction as a temporary dural replacement and at the same time as a biomatrix for dural regeneration.
Publication Date: 2005-06-03 PubMed ID: 15912254DOI: 10.1007/s00701-005-0552-0Google Scholar: Lookup
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- Comparative Study
- Journal Article
Summary
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This study aims to determine the effectiveness of a new collagen biomatrix, sourced from horse collagen, in comparison to a traditional human cadaver-derived dural graft for the repair and regeneration of dural defects. The researchers found that the collagen biomatrix showed promising results in an animal model, with potential for use as both a temporary replacement and a platform for longer-term regeneration of dura.
Methodology
- For this study, dural defects of a defined size were artificially created in 25 sheep. These defects were then repaired with either the collagen biomatrix or the Tutoplast Dura – a traditional dural graft derived from human cadavers. The objective was to investigate how well each material could facilitate the repair and regeneration of the dura mater.
Results
- Observing the sheep post-operation revealed no neurological abnormalities, suggesting that both the collagen graft and the Tutoplast Dura did not negatively impact neurological function in the animals.
- On a cellular level, the collagen graft site showed signs of moderate inflammation and cell infiltration as early as 2 weeks post-surgery, indicating an active biological response. This resulted in the development of a neodura-like connective tissue structure similar to a natural dura.
- On the other hand, Tutoplast Dura showed minimal cellular infiltration and a mild inflammatory response, which suggests it was less biologically active than the collagen graft. It also did not fuse satisfactorily with existing dura and was enclosed in a layer of connective tissue after 24 weeks.
Conclusions
- Taking the results into account, the researchers concluded that the collagen graft appears to be a suitable material for dura reconstruction. It functioned as a temporary dural replacement and, importantly, also served as a biomatrix that supported the regeneration of the dura.
Significance
- The findings of this study represent a significant step forward in the pursuit of optimal materials for dural repair and regeneration. The equine collagen biomatrix demonstrates a viable alternative to traditional human cadaver-derived grafts, potentially overcoming some of the limitations associated with these materials.
Cite This Article
APA
Knopp U, Christmann F, Reusche E, Sepehrnia A.
(2005).
A new collagen biomatrix of equine origin versus a cadaveric dura graft for the repair of dural defects–a comparative animal experimental study.
Acta Neurochir (Wien), 147(8), 877-887.
https://doi.org/10.1007/s00701-005-0552-0 Publication
Researcher Affiliations
- Department of Neurosurgery, University of Lk, Lk, Germany. ulrichknopp@hotmail.com
MeSH Terms
- Animals
- Biocompatible Materials
- Cadaver
- Disease Models, Animal
- Dura Mater / pathology
- Dura Mater / transplantation
- Female
- Fibrillar Collagens
- Horses
- Humans
- Materials Testing
- Sheep
- Transplantation, Heterologous
Citations
This article has been cited 14 times.- Sato M, Oyoshi T, Iwamoto H, Tanoue N, Komasaku S, Higa N, Hosoyama H, Tokimura H, Ibara S, Hanaya R, Yoshimoto K. The collagen matrix dural substitute graft prevents postoperative cerebrospinal fluid leakage after ventriculoperitoneal shunt surgery in patients aged <1 year.. Surg Neurol Int 2022;13:461.
- Mu Y, Shao A, Shi L, Du B, Zhang Y, Luo J, Xu L, Qu S. Immunological Risk Assessment of Xenogeneic Dural Patch by Comparing with Raw Material via GTKO Mice.. Biomed Res Int 2022;2022:7950834.
- Lipovka A, Kharchenko A, Dubovoy A, Filipenko M, Stupak V, Mayorov A, Fomenko V, Geydt P, Parshin D. The Effect of Adding Modified Chitosan on the Strength Properties of Bacterial Cellulose for Clinical Applications.. Polymers (Basel) 2021 Jun 18;13(12).
- Gallo N, Natali ML, Sannino A, Salvatore L. An Overview of the Use of Equine Collagen as Emerging Material for Biomedical Applications.. J Funct Biomater 2020 Nov 1;11(4).
- Kinaci A, Bergmann W, Bleys RL, van der Zwan A, van Doormaal TP. Histologic Comparison of the Dura Mater among Species.. Comp Med 2020 Apr 1;70(2):170-175.
- Kizmazoglu C, Aydin HE, Kaya I, Atar M, Husemoglu B, Kalemci O, Sozer G, Havitcioglu H. Comparison of Biomechanical Properties of Dura Mater Substitutes and Cranial Human Dura Mater : An In Vitro Study.. J Korean Neurosurg Soc 2019 Nov;62(6):635-642.
- Lima FM, Pinto FC, Andrade-da-Costa BL, Silva JG, Campos Júnior O, Aguiar JL. Biocompatible bacterial cellulose membrane in dural defect repair of rat.. J Mater Sci Mater Med 2017 Mar;28(3):37.
- Pogorielov M, Kravtsova A, Reilly GC, Deineka V, Tetteh G, Kalinkevich O, Pogorielova O, Moskalenko R, Tkach G. Experimental evaluation of new chitin-chitosan graft for duraplasty.. J Mater Sci Mater Med 2017 Feb;28(2):34.
- Suwanprateeb J, Luangwattanawilai T, Theeranattapong T, Suvannapruk W, Chumnanvej S, Hemstapat W. Bilayer oxidized regenerated cellulose/poly ε-caprolactone knitted fabric-reinforced composite for use as an artificial dural substitute.. J Mater Sci Mater Med 2016 Jul;27(7):122.
- Wang F, Xue Y, Zhao SS, Yang TJ, Song HQ, Liu H. Suturing-Free Artificial Dura with Dacron Heart Patch in Decompressive Craniectomy and Cranioplasty.. Indian J Surg 2015 Dec;77(Suppl 3):1008-11.
- Al-Ghamdi KB, Bakhsh ZA. Partial glossectomy and floor of mouth (FOM) defect repair with biological dural graft: A case report.. Int J Surg Case Rep 2015;11:78-82.
- Schiariti M, Acerbi F, Broggi M, Tringali G, Raggi A, Broggi G, Ferroli P. Two alternative dural sealing techniques in posterior fossa surgery: (Polylactide-co-glycolide) self-adhesive resorbable membrane versus polyethylene glycol hydrogel.. Surg Neurol Int 2014;5:171.
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