A new method of producing casts for anatomical studies.
Abstract: The objective of the present study was to verify if polyurethane foam is a suitable material to make accurate casts of vessels and viscera, and to develop a method based on its use for anatomical studies. This new technique has been tested primarily on the lungs of different animals, but also on the renal, intestinal and equine digital vessels. It consisted of three steps: specimen preparation, injection of the foam and corrosion of the cast. All structures injected with foam were properly filled. The bronchial tree and the vessels could be observed up to their finer branches. The method is inexpensive, simple and requires no special equipment. The pre-casting procedure does not require perfusion of the specimens with formalin, or prolonged flushing with carbon dioxide gas or air for drying. The polyurethane foam does not need a catalyst. It is simply diluted with acetone, which does not cause shrinkage of the cast due to evaporation during hardening. The foam naturally expands into the cavities without high pressure of the inoculum, and hardens in just 2 or 3 h at room temperature. Only two drawbacks were observed. The first is the fact that multiple injections cannot be made in the same cavity since the foam solidifies quickly; the second is the slight brittleness of the cast, due to the low elasticity of polyurethane foam. In conclusion, polyurethane foam was a suitable material for producing accurate casts of vessels and viscera.
Publication Date: 2014-05-01 PubMed ID: 24788383DOI: 10.1007/s12565-014-0240-3Google Scholar: Lookup
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- Journal Article
Summary
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The research paper explores a new cost-effective and simple method of making accurate casts of vessels and viscera using polyurethane foam that doesn’t require special equipment.
Objective of the Research
- The study was undertaken to evaluate the effectiveness of using polyurethane foam to create anatomical casts. A new technique based on the use of this material was developed, particularly focusing on lungs and vessels of various animals.
Research Methodology
- The technique involved capturing the detailed anatomy of organs and vessels using polyurethane foam, which was conducted in three primary steps:
- Specimen preparation was the first stage of the process. Here, the organ or vessels of interest were prepared for the casting process.
- The second step was the foam injection wherein polyurethane foam was introduced into the prepared specimens.
- The last step was the corrosion of the cast, which helped in obtaining accurate forms of the structures.
Key Findings
- The study discovered that all structures filled with the foam were accurately represented, including the finer branches of the bronchial tree and vessels.
- The use of polyurethane foam proved economical, simple, and didn’t necessitate special equipment or pre-casting procedures like specimen perfusion with formalin, or extensive drying with gas or air. The foam didn’t shrink due to evaporation during the hardening process, contributed by the acetone with which it was diluted.
- The foam expanded naturally into the specimen cavities and solidified within 2 or 3 hours at room temperature.
Challenges
- Despite its success, there were a couple of concerns with the method. Firstly, the quick solidification of the foam did not permit multiple injections in the same cavity.
- The second issue stemmed from the foam’s limited elasticity, resulting in a slightly brittle cast.
Conclusion
- Despite the challenges, the study found polyurethane foam to be an effective material for creating accurate casts of vessels and viscera, owing to its cost-effectiveness, simplicity, and ability to depict minute anatomical details.
Cite This Article
APA
De Sordi N, Bombardi C, Chiocchetti R, Clavenzani P, Trerè C, Canova M, Grandis A.
(2014).
A new method of producing casts for anatomical studies.
Anat Sci Int, 89(4), 255-265.
https://doi.org/10.1007/s12565-014-0240-3 Publication
Researcher Affiliations
- Department of Veterinary Medical Sciences (UNI EN ISO 9001 2008), University of Bologna, Via Tolara di Sopra, 50, 40064, Ozzano dell'Emilia, Bologna, Italy.
MeSH Terms
- Acetone
- Animals
- Cats
- Corrosion Casting / methods
- Dogs
- Formaldehyde
- Horses
- Models, Anatomic
- Polyurethanes
- Time Factors
Citations
This article has been cited 6 times.- Rojo Ríos D, Ramírez Zarzosa G, Soler Laguía M, Kilroy D, Martínez Gomariz F, Sánchez Collado C, Gil Cano F, García García MI, Jáber JR, Arencibia Espinosa A. Creation of Three-Dimensional Anatomical Vascular and Biliary Models for the Study of the Feline Liver (Felis silvestris catus L.): A Comparative CT, Volume Rendering (Vr), Cast and 3D Printing Study.. Animals (Basel) 2023 May 9;13(10).
- Lambertini C, Grandis A, De Silva M, Cassano IA, Checcacci Carboni S, Romagnoli N. The Spritztube: A New Device for the Extraglottic Intubation of Rabbits.. Animals (Basel) 2022 Dec 31;13(1).
- Zhang X, Deng W, Ju J, Zhang S, Wang H, Geng K, Wang D, Zhang G, Le Y, Hou R. A Method to Visualize and Quantify the Intraosseous Arteries of the Femoral Head by Vascular Corrosion Casting.. Orthop Surg 2022 Aug;14(8):1864-1872.
- Martín-Orti R, Tostado-Marcos C, Loureiro JP, Molpeceres-Diego I, Tendillo-Domínguez E, Santos-Álvarez I, Pérez-Lloret P, González-Soriano J. The Digestive System of the Arctocephalus australis in Comparison to the Dog as a Land-Carnivore Model.. Animals (Basel) 2022 Jun 25;12(13).
- Santoro F, Franci P, Grandis A, Valgimigli S. Distribution of injectates in the thoracic paravertebral space of the dog and cat: A cadaveric study.. Open Vet J 2021 Jan-Mar;11(1):27-35.
- Shanthini S, Suma HY. An innovative method in venous coronary cast technique.. Anat Cell Biol 2019 Jun;52(2):191-195.
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