A preliminary study of (13)c-phenylalanine and (13)c-dipeptide breath tests in horses.
Abstract: This study aimed to establish a standard dose and sample collection time for (13)C phenylalanine and (13)C-Dipeptide breath test in horses. To evaluate dose-dependent effects, healthy horses received 2.5 mg/kg, 5 mg/kg, and 10 mg/kg (13)C phenylalanine dissolved in 1 ml/kg distilled water and 1.25 mg/kg, 2.5 mg/kg, and 5 mg/kg (13)C dipeptide dissolved in 2 ml/ kg distilled water. Tmax was observed during the sample collection time. For (13)C phenylalanine, the standard deviation of Cmax at 5 mg/kg was lower than that of 10 mg/kg. For (13)C dipeptide, the standard deviation of Tmax was the lowest at 5 mg/kg. This study revealed that an optimal dose for breath tests with (13)C phenylalanine and (13)C dipeptide may be 5 mg/kg in horses.
Publication Date: 2009-04-17 PubMed ID: 24833963PubMed Central: PMC4013957DOI: 10.1294/jes.20.7Google Scholar: Lookup
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Summary
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The research article details a preliminary study on the determination of ideal dosage and sample collection timings for breath tests using (13)C phenylalanine and (13)C-Dipeptide in horses.
Study Overview
- The study aimed to find out the standard dosage and the best time to collect samples for (13)C phenylalanine and (13)C-Dipeptide breath tests in horses.
- Apart from establishing the standard dosage, the researchers also wanted to observe any dose-dependent effects.
- To achieve their goal, they selected healthy horses for the experiment.
Experiment Process
- These horses were given doses of 2.5 mg/kg, 5 mg/kg, and 10 mg/kg of (13)C phenylalanine which was dissolved in 1 ml/kg of distilled water.
- Simultaneously, doses of 1.25 mg/kg, 2.5 mg/kg, and 5 mg/kg of (13)C dipeptide, dissolved in 2 ml/kg of distilled water, were also administered.
- After administering these dosages, Tmax, the time at which the maximum concentration of the test compound in the bloodstream is observed, was recorded during the sample collection period.
Results and Conclusion
- For the (13)C phenylalanine, it was observed that the standard deviation of Cmax (the maximum or peak concentration that a drug achieves in a specified compartment or test area of the body after the drug has been administrated and prior to the administration of a second dose) at 5 mg/kg was lower than that of 10 mg/kg.
- When it came to (13)C dipeptide, it was found that the standard deviation of Tmax was lowest at a dose of 5 mg/kg.
- Basing on these results, the research concluded that the optimal dose for breath tests with (13)C phenylalanine and (13)C dipeptide in horses may be 5 mg/kg.
Cite This Article
APA
Sasaki N, Tsuzuki N, Yamada M, Minami T, Yamada H.
(2009).
A preliminary study of (13)c-phenylalanine and (13)c-dipeptide breath tests in horses.
J Equine Sci, 20(1), 7-10.
https://doi.org/10.1294/jes.20.7 Publication
Researcher Affiliations
- Department of Clinical Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido 080-8555, Japan.
- Department of Clinical Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido 080-8555, Japan.
- Department of Clinical Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido 080-8555, Japan.
- Department of Clinical Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido 080-8555, Japan.
- Department of Clinical Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido 080-8555, Japan.
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