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Home / Equine Research Bank / Vet Res Commun / A reagin-like antibody in horse serum: 1. Occurrence and som...
Veterinary research communications1983; 6(1); 13-23; doi: 10.1007/BF02214891

A reagin-like antibody in horse serum: 1. Occurrence and some biological properties.

Abstract: The demonstration of a reagin-like antibody against Culicoides pulicaris extract in the serum of horses and ponies affected with recurrent seasonal dermatitis (sweet itch) is reported. This antibody can confer Prauznitz-Küstner (P-K) sensitivity on homologous skin for up to 5 days and, like human IgE, is thermolabile and susceptible to 2-mercaptoethanol reduction. It is eluted on diethylaminoethyl dextran-52 anion exchange chromatography independently of IgG, IgG(T) and IgM, and its elution characteristics indicate similarity in net molecular charge to human IgE. The P-K response observed in horse skin is biphasic, and is morphologically similar to the late cutaneous anaphylactic response in man. Both phases of the P-K response are dependent upon the reagin-like antibody, although other serum factors appear involved in the delayed phase of the response.
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Publication Date: 1983-01-01 PubMed ID: 6868343DOI: 10.1007/BF02214891Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper details a study on a reagin-like antibody in the blood of horses and ponies affected with recurrent seasonal dermatitis (or sweet itch). Certain biological properties of this antibody, such as its ability to make the skin sensitive and its structural similarities to human IgE, a type of antibody, were observed and reported.

Occurrence of Reagin-like Antibody in Horse Serum

  • The research opens with the finding of a reagin-like antibody, which is a particular type of immunoglobulin or protein used by the immune system to combat pathogens, in the serum of horses and ponies. These animals were already suffering from recurrent seasonal dermatitis, a skin condition often called “sweet itch”.
  • These antibodies have been shown to induce a specific type of sensitivity in the skin of the same species for up to five days. This is referred to as the Prauznitz-Küstner (P-K) sensitivity.

Biological Properties

  • The reagin-like antibody demonstrated qualities similar to those of human IgE, which is another class of antibodies in the immune system essential for allergy reactions.
  • It was observed to be both thermolabite (sensitive to heat) and susceptible to reduction by 2-mercaptoethanol. The latter is a process frequently employed in labs to break the disulphide bonds in proteins. This sensitivity and susceptibility suggest a certain structural similarity with human IgE.
  • This antibody is extracted or “eluted” through a method independent of IgG, IgG(T) and IgM, which are all other types of antibodies. This indicates that the reagin-like antibody possesses distinctive characteristics.
  • The elution characteristics of this antibody suggest that its charge properties are similar to human IgE.

P-K Response in Horse Skin

  • The response in the horse skin after the introduction of this antibody is considered biphasic, meaning it occurs in two distinct stages.
  • Both stages are dependent on the introduction of this reagin-like antibody. However, the research also identifies the likely involvement of other serum factors in the delayed phase of the response, suggesting a complex mechanism underlying this immune reaction.

Cite This Article

APA
Matthews AG, Imlah P, McPherson EA. (1983). A reagin-like antibody in horse serum: 1. Occurrence and some biological properties. Vet Res Commun, 6(1), 13-23. https://doi.org/10.1007/BF02214891

Publication

Veterinary research communications
ISSN: 0165-7380
NlmUniqueID: 8100520
Country: Switzerland
Language: English
Volume: 6
Issue: 1
Pages: 13-23

Researcher Affiliations

Matthews, A G
    Imlah, P
      McPherson, E A

        MeSH Terms

        • Animals
        • Antibodies / analysis
        • Antibodies / isolation & purification
        • Ceratopogonidae / immunology
        • Chemical Phenomena
        • Chemistry
        • Chromatography, DEAE-Cellulose
        • Chromatography, Ion Exchange
        • Dermatitis, Atopic / immunology
        • Dermatitis, Atopic / veterinary
        • Horse Diseases / immunology
        • Horses
        • Immunoglobulin E / isolation & purification
        • Intradermal Tests
        • Reagins / analysis
        • Recurrence
        • Seasons
        • Skin / immunology

        References

        This article includes 18 references
        1. Mellor PS, McCraig J. The probable cause of "sweet itch" in England.. Vet Rec 1974 Nov 2;95(18):411-5.
          pubmed: 4451336doi: 10.1136/vr.95.18.411google scholar: lookup
        2. Dolovich J, Hargreave FE, Chalmers R, Shier KJ, Gauldie J, Bienenstock J. Late cutaneous allergic responses in isolated IgE-dependent reactions.. J Allergy Clin Immunol 1973 Jul;52(1):38-46.
          pubmed: 4577089doi: 10.1016/0091-6749(73)90119-xgoogle scholar: lookup
        3. KUHNS WJ. Disappearance of human diphtheria antitoxin from human passive transfer skin sites.. Proc Soc Exp Biol Med 1961 Oct;108:63-7.
          pubmed: 14460352doi: 10.3181/00379727-108-26845google scholar: lookup
        4. Dorrington KJ, Bennich H. Thermally induced structural changes in immunoglobulin E.. J Biol Chem 1973 Dec 25;248(24):8378-84.
          pubmed: 4128478
        5. McPherson EA, Lawson GH, Murphy JR, Nicholson JM, Breeze RG, Pirie HM. Chronic obstructive pulmonary disease (COPD) in horses: aetiological studies: responses to intradermal and inhalation antigenic challenge.. Equine Vet J 1979 Jul;11(3):159-66.
          pubmed: 385306doi: 10.1111/j.2042-3306.1979.tb01330.xgoogle scholar: lookup
        6. Johansson SG, Bennich H, Wide L. A new class of immunoglobulin in human serum.. Immunology 1968 Feb;14(2):265-72.
          pubmed: 4170889
        7. Umemoto L, Poothullil J, Dolovich J, Hargreave FE. Factors which influence late cutaneous allergic responses.. J Allergy Clin Immunol 1976 Jul;58(1 PT 1):60-8.
          pubmed: 947978doi: 10.1016/0091-6749(76)90107-xgoogle scholar: lookup
        8. Allen PZ, Dalton EJ. Studies on equine immunoglobulins. IV. Immunoglobulins of the donkey.. Immunology 1975 Jan;28(1):187-97.
          pubmed: 1116869
        9. Halliwell RE, Fleischman JB, Mackay-Smith M, Beech J, Gunson DE. The role of allergy in chronic pulmonary disease of horses.. J Am Vet Med Assoc 1979 Feb 1;174(3):277-81.
          pubmed: 571867
        10. Bryant DH, Burns MW, Lazarus L. Identification of IgG antibody as a carrier of reaginic activity in asthmatic patients.. J Allergy Clin Immunol 1975 Dec;56(6):417-28.
          pubmed: 812894doi: 10.1016/0091-6749(75)90060-3google scholar: lookup
        11. Eyre P. Anaphylactic (skin-sensitising) antibodies in the horse.. Vet Rec 1972 Jan 8;90(2):36-7.
          pubmed: 5014724doi: 10.1136/vr.90.2.36google scholar: lookup
        12. Perelmutter L, Liakopoulou A. Detection of IgE mediated immediate hypersensitivity reactions in the sera of ragweed sensitive individuals using rat mast cells.. Int Arch Allergy Appl Immunol 1971;40(4-5):481-94.
          pubmed: 4102841doi: 10.1159/000230432google scholar: lookup
        13. Nielsen KH. Bovine reaginic antibody III. Cross-reaction of antihuman IgE and antibovine reaginic immunoglobulin antisera with sera from several species of mammals.. Can J Comp Med 1977 Jul;41(3):345-8.
          pubmed: 907910
        14. Devey ME, Wilson DV, Wheeler AW. The IgG subclasses of antibodies to grass pollen allergens produced in hay fever patients during hyposensitization.. Clin Allergy 1976 May;6(3):227-36.
          pubmed: 939035doi: 10.1111/j.1365-2222.1976.tb01901.xgoogle scholar: lookup
        15. Schatzmann U, de Weck AL, Gerber H, Lazàry S, Straub R, Pauli B. Active and passive cutaneous anaphylaxis in the horse following immunization with benzylpenicilloyl-bovine gamma globulin (BPO20-BGG).. Res Vet Sci 1973 Nov;15(3):347-52.
          pubmed: 4134593
        16. Halliwell RE, Schwartzman RM, Rockey JH. Antigenic relationship between human IgE and canine IgE.. Clin Exp Immunol 1972 Mar;10(3):399-407.
          pubmed: 4624496
        17. Cordal ME, Margni RA. Isolation, purification and biological properties of horse precipitating and non precipitating antibodies.. Immunochemistry 1974 Dec;11(12):765-70.
          pubmed: 4142779doi: 10.1016/0019-2791(74)90295-xgoogle scholar: lookup
        18. Ishizaka K, Ishizaka T. Identification of gamma-E-antibodies as a carrier of reaginic activity.. J Immunol 1967 Dec;99(6):1187-98.
          pubmed: 4168663

        Citations

        This article has been cited 4 times.
        1. Larson EM, Babasyan S, Wagner B. Phenotype and function of IgE-binding monocytes in equine Culicoides hypersensitivity.. PLoS One 2020;15(5):e0233537.
          doi: 10.1371/journal.pone.0233537pubmed: 32442209google scholar: lookup
        2. Morán G, Burgos R, Araya O, Folch H. In vitro bioassay to detect reaginic antibodies from the serum of horses affected with recurrent airway obstruction.. Vet Res Commun 2010 Jan;34(1):91-9.
          doi: 10.1007/s11259-009-9334-0pubmed: 20012188google scholar: lookup
        3. Foster AP, Lees P, Cunningham FM. Actions of PAF receptor antagonists in horses with the allergic skin disease sweet itch.. Inflamm Res 1995 Oct;44(10):412-7.
          doi: 10.1007/BF01757697pubmed: 8564516google scholar: lookup
        4. Matthews AG, Imlah P, McPherson EA. A reagin-like antibody in horse serum. II. Anti-human IgE induced reversed cutaneous anaphylaxis-like responses in horse skin.. Vet Res Commun 1983 Mar;6(2):111-22.
          doi: 10.1007/BF02214903pubmed: 6603051google scholar: lookup
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