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Home / Equine Research Bank / Int J Mol Sci / A Satellite-Free Centromere in Equus przewalskii Chromosome ...
International journal of molecular sciences2023; 24(4); doi: 10.3390/ijms24044134

A Satellite-Free Centromere in Equus przewalskii Chromosome 10.

Abstract: In mammals, centromeres are epigenetically specified by the histone H3 variant CENP-A and are typically associated with satellite DNA. We previously described the first example of a natural satellite-free centromere on Equus caballus chromosome 11 (ECA11) and, subsequently, on several chromosomes in other species of the genus Equus. We discovered that these satellite-free neocentromeres arose recently during evolution through centromere repositioning and/or chromosomal fusion, after inactivation of the ancestral centromere, where, in many cases, blocks of satellite sequences were maintained. Here, we investigated by FISH the chromosomal distribution of satellite DNA families in Equus przewalskii (EPR), demonstrating a good degree of conservation of the localization of the major horse satellite families 37cen and 2PI with the domestic horse. Moreover, we demonstrated, by ChIP-seq, that 37cen is the satellite bound by CENP-A and that the centromere of EPR10, the ortholog of ECA11, is devoid of satellite sequences. Our results confirm that these two species are closely related and that the event of centromere repositioning which gave rise to EPR10/ECA11 centromeres occurred in the common ancestor, before the separation of the two horse lineages.
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Publication Date: 2023-02-18 PubMed ID: 36835543PubMed Central: PMC9961726DOI: 10.3390/ijms24044134Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article explores the unique aspect of certain chromosomes in the Equus przewalskii species, specifically chromosome 10, which unusually carries a satellite-free centromere.

Overview of the Study

  • The researchers’ objective was to understand the distribution of satellite DNA families in the Equus przewalskii species. As part of their investigation, they performed Fluorescence In Situ Hybridization (FISH).
  • The study recognized that the histone H3 variant CENP-A epigenetically describes centromeres in mammals, which are generally connected with satellite DNA. They sought to scrutinize Equus przewalskii chromosome 10 (EPR10), which defied this norm by having a centromere free of satellite DNA.
  • In previous studies, such satellite-free neocentromeres were found on chromosome 11 (ECA11) and on numerous chromosomes in different Equus species. The researchers utilized their understanding from these previous investigations.

Findings and Implications

  • The study confirmed that Equus przewalskii maintains a significant level of conservation concerning the locality of major horse satellite families 37cen and 2PI, as compared to the domestic horse.
  • CENP-A, the identified satellite bound to 37cen, was found through Chromatin Immunoprecipitation sequencing (ChIP-seq) method. It was observed that the centromere of EPR10 is lacking satellite sequences, making it different from the norm.
  • These outcomes support the close relation between these two Equus species. The repositioning event that resulted in the EPR10 and ECA11 centromeres likely happened in the common ancestor before the two horse lineages split.
  • The study adds to the field’s understanding of chromosome and centromere evolution and diversity. By highlighting the existence and creation of satellite-free centromeres, the research plays a vital role in advancing scientific knowledge in genetics, evolution, and potential biomedical applications.

Cite This Article

APA
Piras FM, Cappelletti E, Abdelgadir WA, Salamon G, Vignati S, Santagostino M, Sola L, Nergadze SG, Giulotto E. (2023). A Satellite-Free Centromere in Equus przewalskii Chromosome 10. Int J Mol Sci, 24(4). https://doi.org/10.3390/ijms24044134

Publication

International journal of molecular sciences
ISSN: 1422-0067
NlmUniqueID: 101092791
Country: Switzerland
Language: English
Volume: 24
Issue: 4

Researcher Affiliations

Piras, Francesca M
  • Department of Biology and Biotechnology "Lazzaro Spallanzani", University of Pavia, 27100 Pavia, Italy.
Cappelletti, Eleonora
  • Department of Biology and Biotechnology "Lazzaro Spallanzani", University of Pavia, 27100 Pavia, Italy.
Abdelgadir, Wasma A
  • Department of Biology and Biotechnology "Lazzaro Spallanzani", University of Pavia, 27100 Pavia, Italy.
Salamon, Giulio
  • Oasi di Sant'Alessio, Sant'Alessio con Vialone, 27016 Pavia, Italy.
Vignati, Simone
  • Independent Researcher, 27100 Pavia, Italy.
Santagostino, Marco
  • Department of Biology and Biotechnology "Lazzaro Spallanzani", University of Pavia, 27100 Pavia, Italy.
Sola, Lorenzo
  • Department of Biology and Biotechnology "Lazzaro Spallanzani", University of Pavia, 27100 Pavia, Italy.
Nergadze, Solomon G
  • Department of Biology and Biotechnology "Lazzaro Spallanzani", University of Pavia, 27100 Pavia, Italy.
Giulotto, Elena
  • Department of Biology and Biotechnology "Lazzaro Spallanzani", University of Pavia, 27100 Pavia, Italy.

MeSH Terms

  • Animals
  • Centromere / metabolism
  • Centromere Protein A / metabolism
  • DNA, Satellite
  • Horses / genetics

Grant Funding

  • Dipartimenti di Eccellenza Program (2018-2022)-Department of Biology and Biotechnology "L. Spallanzani", University of Pavia / the Italian Ministry of Education, University and Research (MIUR)
  • Grant 2019-67015-29340/Project Accession 1018854 / USDA National Institute of Food and Agriculture.

Conflict of Interest Statement

The authors declare no conflict of interest.

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