A serological study of severe fever with thrombocytopenia syndrome using a virus neutralization test and competitive enzyme-linked immunosorbent assay.
Abstract: Severe fever with thrombocytopenia syndrome (SFTS) is caused by the SFTS virus (SFTSV). The SFTSV appears to have a wide host range, as SFTSV-positive ticks have been isolated from both farm animals and wild rodents. Therefore, it is important to monitor SFTSV-positive animals to prevent the transmission of SFTSV from animals to humans. Previously, we developed a competitive enzyme-linked immunosorbent assay (cELISA) to detect SFTSV-specific antibodies from field animals and compared the cELISA results to those from an indirect immunofluorescence assay (IFA). In this study, cELISA results were compared to and evaluated against the results from both an IFA and a virus neutralization (VN) test of 193 bovine serum samples (including two bovine positive control sera) and 70 horse serum samples. The consistency (98.9%) between cELISA and VN results was higher than that (97.4%) between cELISA and IFA for the bovine serum samples. Similarly, for the horse serum samples, the consistency (88.6%) between cELISA and VN results was higher than that (84.3%) between the cELISA and IFA. These findings indicate that our newly developed cELISA can be used for surveillance or epidemiological studies of SFTSV in animals.
Publication Date: 2016-06-15 PubMed ID: 27297411PubMed Central: PMC5366300DOI: 10.4142/jvs.2017.18.1.33Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Comparative Study
- Journal Article
- Animal Health
- Animal Models
- Antibodies
- Comparative Study
- Diagnostic Technique
- Disease Diagnosis
- Disease Surveillance
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Epidemiology
- Equine Health
- Horses
- Immunofluorescence Assay
- Infectious Disease
- Public Health
- Serological Surveys
- Serology
- Seroprevalence
- Veterinary Medicine
- Veterinary Research
- Virus
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The study focuses on the application of a method called competitive enzyme-linked immunosorbent assay (cELISA) in detecting severe fever with thrombocytopenia syndrome virus (SFTSV) in animal samples. The results of the study indicate that the cELISA method demonstrates high consistency and can be effectively used for researching and monitoring SFTSV in animals.
Objective and Method of the Study
- The primary purpose of this study is to monitor the transmission of the severe fever with thrombocytopenia syndrome virus (SFTSV) from animals to humans through the use of a diagnostic test called competitive enzyme-linked immunosorbent assay (cELISA).
- The researchers previously developed the cELISA method to detect SFTSV-specific antibodies from animals and are now comparing the results of this method with the results of two other tests: the indirect immunofluorescence assay (IFA) and the virus neutralization (VN) test.
Sample and Findings
- The research team analyzed a total of 263 animal serum samples which included 193 bovine serum samples (two of which were positive controls) and 70 horse serum samples.
- The results of their analysis demonstrated that the cELISA method for detecting SFTSV antibodies showed a high rate of consistency with the VN test, more so than with the IFA test. This consistency was 98.9% for bovine samples and 88.6% for horse samples when comparing cELISA and VN results.
Implications of the Research
- This research provides evidence that the cELISA method could be a valid tool for monitoring and surveying animals for SFTSV, helping to prevent its transmission to humans.
- The application of cELISA for these purposes could contribute to the broader efforts in controlling and preventing the spread of the SFTSV.
Cite This Article
APA
Lee H, Kim EJ, Cho IS, Song JY, Choi JS, Lee JY, Shin YK.
(2016).
A serological study of severe fever with thrombocytopenia syndrome using a virus neutralization test and competitive enzyme-linked immunosorbent assay.
J Vet Sci, 18(1), 33-38.
https://doi.org/10.4142/jvs.2017.18.1.33 Publication
Researcher Affiliations
- Viral Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.
- Viral Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.
- Viral Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.
- Viral Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.
- Foreign Animal Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.
- Foreign Animal Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.
- Viral Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.
MeSH Terms
- Animals
- Cattle
- Cattle Diseases / diagnosis
- Cattle Diseases / virology
- Enzyme-Linked Immunosorbent Assay / veterinary
- Fluorescent Antibody Technique, Indirect / veterinary
- Horse Diseases / diagnosis
- Horse Diseases / virology
- Horses
- Neutralization Tests / veterinary
- Phlebotomus Fever / diagnosis
- Phlebotomus Fever / veterinary
- Phlebotomus Fever / virology
- Phlebovirus / isolation & purification
- Thrombocytopenia / diagnosis
- Thrombocytopenia / veterinary
- Thrombocytopenia / virology
Conflict of Interest Statement
The authors declare no conflicts of interest.
References
This article includes 22 references
- Ammerman NC, Beier-Sexton M, Azad AF. Growth and maintenance of Vero cell lines.. Curr Protoc Microbiol 2008 Nov;Appendix 4:Appendix 4E.
- Bosco-Lauth AM, Panella NA, Root JJ, Gidlewski T, Lash RR, Harmon JR, Burkhalter KL, Godsey MS, Savage HM, Nicholson WL, Komar N, Brault AC. Serological investigation of heartland virus (Bunyaviridae: Phlebovirus) exposure in wild and domestic animals adjacent to human case sites in Missouri 2012-2013.. Am J Trop Med Hyg 2015 Jun;92(6):1163-7.
- Cliquet F, Aubert M, Sagné L. Development of a fluorescent antibody virus neutralisation test (FAVN test) for the quantitation of rabies-neutralising antibody.. J Immunol Methods 1998 Mar 1;212(1):79-87.
- Doan HT, Noh JH, Choe SE, Yoo MS, Kim YH, Reddy KE, Quyen DV, Nguyen LT, Nguyen TT, Kweon CH, Jung SC, Chang KY, Kang SW. Molecular detection and phylogenetic analysis of Anaplasma bovis from Haemaphysalis longicornis feeding on grazing cattle in Korea.. Vet Parasitol 2013 Sep 23;196(3-4):478-81.
- Guo X, Zhang L, Zhang W, Chi Y, Zeng X, Li X, Qi X, Jin Q, Zhang X, Huang M, Wang H, Chen Y, Bao C, Hu J, Liang S, Bao L, Wu T, Zhou M, Jiao Y. Human antibody neutralizes severe Fever with thrombocytopenia syndrome virus, an emerging hemorrhagic Fever virus.. Clin Vaccine Immunol 2013 Sep;20(9):1426-32.
- Jiao Y, Zeng X, Guo X, Qi X, Zhang X, Shi Z, Zhou M, Bao C, Zhang W, Xu Y, Wang H. Preparation and evaluation of recombinant severe fever with thrombocytopenia syndrome virus nucleocapsid protein for detection of total antibodies in human and animal sera by double-antigen sandwich enzyme-linked immunosorbent assay.. J Clin Microbiol 2012 Feb;50(2):372-7.
- Kim CM, Kim MS, Park MS, Park JH, Chae JS. Identification of Ehrlichia chaffeensis, Anaplasma phagocytophilum, and A. bovis in Haemaphysalis longicornis and Ixodes persulcatus ticks from Korea.. Vector Borne Zoonotic Dis 2003 Spring;3(1):17-26.
- Kim KH, Yi J, Kim G, Choi SJ, Jun KI, Kim NH, Choe PG, Kim NJ, Lee JK, Oh MD. Severe fever with thrombocytopenia syndrome, South Korea, 2012.. Emerg Infect Dis 2013 Nov;19(11):1892-4.
- Landis JR, Koch GG. An application of hierarchical kappa-type statistics in the assessment of majority agreement among multiple observers.. Biometrics 1977 Jun;33(2):363-74.
- Lee H, Kim EJ, Song JY, Choi JS, Lee JY, Cho IS, Shin YK. Development and evaluation of a competitive enzyme-linked immunosorbent assay using a monoclonal antibody for diagnosis of severe fever with thrombocytopenia syndrome virus in bovine sera.. J Vet Sci 2016 Sep 30;17(3):307-14.
- Lei XY, Liu MM, Yu XJ. Severe fever with thrombocytopenia syndrome and its pathogen SFTSV.. Microbes Infect 2015 Feb;17(2):149-54.
- Libeau G, Diallo A, Calvez D, Lefèvre PC. A competitive ELISA using anti-N monoclonal antibodies for specific detection of rinderpest antibodies in cattle and small ruminants.. Vet Microbiol 1992 Jun 1;31(2-3):147-60.
- McMullan LK, Folk SM, Kelly AJ, MacNeil A, Goldsmith CS, Metcalfe MG, Batten BC, Albariño CG, Zaki SR, Rollin PE, Nicholson WL, Nichol ST. A new phlebovirus associated with severe febrile illness in Missouri.. N Engl J Med 2012 Aug 30;367(9):834-41.
- Niu G, Li J, Liang M, Jiang X, Jiang M, Yin H, Wang Z, Li C, Zhang Q, Jin C, Wang X, Ding S, Xing Z, Wang S, Bi Z, Li D. Severe fever with thrombocytopenia syndrome virus among domesticated animals, China.. Emerg Infect Dis 2013 May;19(5):756-63.
- Park SW, Song BG, Shin EH, Yun SM, Han MG, Park MY, Park C, Ryou J. Prevalence of severe fever with thrombocytopenia syndrome virus in Haemaphysalis longicornis ticks in South Korea.. Ticks Tick Borne Dis 2014 Oct;5(6):975-7.
- Swei A, Russell BJ, Naccache SN, Kabre B, Veeraraghavan N, Pilgard MA, Johnson BJ, Chiu CY. The genome sequence of Lone Star virus, a highly divergent bunyavirus found in the Amblyomma americanum tick.. PLoS One 2013;8(4):e62083.
- Takahashi T, Maeda K, Suzuki T, Ishido A, Shigeoka T, Tominaga T, Kamei T, Honda M, Ninomiya D, Sakai T, Senba T, Kaneyuki S, Sakaguchi S, Satoh A, Hosokawa T, Kawabe Y, Kurihara S, Izumikawa K, Kohno S, Azuma T, Suemori K, Yasukawa M, Mizutani T, Omatsu T, Katayama Y, Miyahara M, Ijuin M, Doi K, Okuda M, Umeki K, Saito T, Fukushima K, Nakajima K, Yoshikawa T, Tani H, Fukushi S, Fukuma A, Ogata M, Shimojima M, Nakajima N, Nagata N, Katano H, Fukumoto H, Sato Y, Hasegawa H, Yamagishi T, Oishi K, Kurane I, Morikawa S, Saijo M. The first identification and retrospective study of Severe Fever with Thrombocytopenia Syndrome in Japan.. J Infect Dis 2014 Mar;209(6):816-27.
- Yu L, Zhang L, Sun L, Lu J, Wu W, Li C, Zhang Q, Zhang F, Jin C, Wang X, Bi Z, Li D, Liang M. Critical epitopes in the nucleocapsid protein of SFTS virus recognized by a panel of SFTS patients derived human monoclonal antibodies.. PLoS One 2012;7(6):e38291.
- Yu XJ, Liang MF, Zhang SY, Liu Y, Li JD, Sun YL, Zhang L, Zhang QF, Popov VL, Li C, Qu J, Li Q, Zhang YP, Hai R, Wu W, Wang Q, Zhan FX, Wang XJ, Kan B, Wang SW, Wan KL, Jing HQ, Lu JX, Yin WW, Zhou H, Guan XH, Liu JF, Bi ZQ, Liu GH, Ren J, Wang H, Zhao Z, Song JD, He JR, Wan T, Zhang JS, Fu XP, Sun LN, Dong XP, Feng ZJ, Yang WZ, Hong T, Zhang Y, Walker DH, Wang Y, Li DX. Fever with thrombocytopenia associated with a novel bunyavirus in China.. N Engl J Med 2011 Apr 21;364(16):1523-32.
- Yun SM, Lee WG, Ryou J, Yang SC, Park SW, Roh JY, Lee YJ, Park C, Han MG. Severe fever with thrombocytopenia syndrome virus in ticks collected from humans, South Korea, 2013.. Emerg Infect Dis 2014 Aug;20(8):1358-61.
- Zhao L, Zhai S, Wen H, Cui F, Chi Y, Wang L, Xue F, Wang Q, Wang Z, Zhang S, Song Y, Du J, Yu XJ. Severe fever with thrombocytopenia syndrome virus, Shandong Province, China.. Emerg Infect Dis 2012 Jun;18(6):963-5.
- Zweig MH, Campbell G. Receiver-operating characteristic (ROC) plots: a fundamental evaluation tool in clinical medicine.. Clin Chem 1993 Apr;39(4):561-77.
Citations
This article has been cited 0 times.Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
