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Home / Equine Research Bank / BMC Cell Biol / A simplified but robust method for the isolation of avian an...
BMC cell biology2012; 13; 16; doi: 10.1186/1471-2121-13-16

A simplified but robust method for the isolation of avian and mammalian muscle satellite cells.

Abstract: Current methods of isolation of muscle satellite cells from different animal species are highly variable making inter-species comparisons problematic. This variation mainly stems from the use of different proteolytic enzymes to release the satellite cells from the muscle tissue (sometimes a single enzyme is used but often a combination of enzymes is preferred) and the different extracellular matrix proteins used to coat culture ware. In addition, isolation of satellite cells is frequently laborious and sometimes may require pre-plating of the cell preparation on uncoated flasks or Percoll centrifugation to remove contaminating fibroblasts. The methodology employed to isolate and culture satellite cells in vitro can critically determine the fusion of myoblasts into multi-nucleated myotubes. These terminally differentiated myotubes resemble mature myofibres in the muscle tissue in vivo, therefore optimal fusion is a keystone of in vitro muscle culture. Hence, a simple method of muscle satellite cell isolation and culture of different vertebrate species that can result in a high fusion rate is highly desirable. Results: We demonstrate here a relatively simple and rapid method of isolating highly enriched muscle satellite cells from different avian and mammalian species. In brief, muscle tissue was mechanically dissociated, digested with a single enzyme (pronase), triturated with a 10-ml pipette, filtered and directly plated onto collagen coated flasks. Following this method and after optimization of the cell culture conditions, excellent fusion rates were achieved in the duck, chicken, horse and cow (with more than 50% cell fusion), and to a lesser extent pig, pointing to pronase as a highly suitable enzyme to release satellite cells from muscle tissue. Conclusions: Our simplified method presents a quick and simple alternative to isolating highly enriched muscle satellite cell cultures which can subsequently rapidly differentiate into well developed primary myotubes. The use of the same isolation protocol allows better inter-species comparisons of muscle satellite cells. Of all the farm animal species investigated, harvested chicken muscle cells showed the highest percentage of muscle satellite cells, and equine muscle cells presented the highest fusion index, an impressive ≈ 77%. Porcine cells displayed the lowest amount of satellite cells but still achieved a modest fusion rate of ≈ 41%.
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Publication Date: 2012-06-21 PubMed ID: 22720831PubMed Central: PMC3432597DOI: 10.1186/1471-2121-13-16Google Scholar: Lookup
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  • Journal Article
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article presents a simplified and efficient method for isolating muscle satellite cells from various avian and mammalian species, contributing to better inter-species comparisons and understanding of muscle cell biology.

Introduction and Background

  • The study is a response to the inconsistency in isolation methods of muscle satellite cells across various animal species, which has created difficulties in inter-species comparisons.
  • Challenges and variations in the isolation process are attributed to different proteolytic enzymes used to separate satellite cells from muscle tissue and various extracellular matrix proteins used in coating culture ware.
  • The methods employed in isolating and culturing these cells can determine the fusion of myoblasts into multi-nucleated myotubes. Hence, the authors of the research stress the need for a simplified isolation method that ensures a high fusion rate.

Methodology and Results

  • The research outlines an efficient method for isolating highly enriched muscle satellite cells from avian and mammalian species. The muscle tissue was mechanically dissociated, digested with a single enzyme (pronase), triturated with a 10-ml pipette, filtered, and directly plated onto collagen coated flasks.
  • Following the outlined method, high fusion rates were achieved in duck, chicken, horse and cow. Also, a lesser but significant fusion rate was achieved in pigs.
  • Resultantly, the findings highlight pronase as a very suitable enzyme for releasing satellite cells from muscle tissue.

Conclusions and Implications

  • The presented method provides a straightforward and effective alternative to current problematic methods. It also facilitates the establishment of enriched muscle satellite cell cultures which could rapidly differentiate into well-developed primary myotubes.
  • Using this unified protocol enhances the capacity to make better inter-species comparisons of muscle satellite cells, thus improving our understanding of muscle biology in different species.
  • Among the farm animal species investigated, chicken muscle cells had the highest percentage of muscle satellite cells, equine muscle cells presented the highest fusion index, while pig cells displayed the lowest amount of satellite cells but still had a reasonable fusion rate.

Cite This Article

APA
Baquero-Perez B, Kuchipudi SV, Nelli RK, Chang KC. (2012). A simplified but robust method for the isolation of avian and mammalian muscle satellite cells. BMC Cell Biol, 13, 16. https://doi.org/10.1186/1471-2121-13-16

Publication

BMC cell biology
ISSN: 1471-2121
NlmUniqueID: 100966972
Country: England
Language: English
Volume: 13
Pages: 16

Researcher Affiliations

Baquero-Perez, Belinda
  • School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, College Road, Loughborough, Leicestershire, LE12 5RD, UK. svxbb@exmail.nottingham.ac.uk
Kuchipudi, Suresh V
    Nelli, Rahul K
      Chang, Kin-Chow

        MeSH Terms

        • Animals
        • Cattle
        • Cell Differentiation
        • Cell Separation
        • Cells, Cultured
        • Chickens
        • Desmin / metabolism
        • Ducks
        • Horses
        • Muscle Fibers, Skeletal / metabolism
        • Myoblasts / metabolism
        • PAX7 Transcription Factor / metabolism
        • Pronase / metabolism
        • Satellite Cells, Skeletal Muscle / cytology
        • Satellite Cells, Skeletal Muscle / metabolism
        • Swine

        Grant Funding

        • BB/F018487/1 / Biotechnology and Biological Sciences Research Council

        References

        This article includes 51 references
        1. Campion DR. The muscle satellite cell: a review.. Int Rev Cytol 1984;87:225-51.
          pubmed: 6370890doi: 10.1016/s0074-7696(08)62444-4google scholar: lookup
        2. MAURO A. Satellite cell of skeletal muscle fibers.. J Biophys Biochem Cytol 1961 Feb;9(2):493-5.
          doi: 10.1083/jcb.9.2.493pmc: PMC2225012pubmed: 13768451google scholar: lookup
        3. Hill M, Wernig A, Goldspink G. Muscle satellite (stem) cell activation during local tissue injury and repair.. J Anat 2003 Jul;203(1):89-99.
          doi: 10.1046/j.1469-7580.2003.00195.xpmc: PMC1571137pubmed: 12892408google scholar: lookup
        4. Winchester PK, Davis ME, Alway SE, Gonyea WJ. Satellite cell activation in the stretch-enlarged anterior latissimus dorsi muscle of the adult quail.. Am J Physiol 1991 Feb;260(2 Pt 1):C206-12.
          pubmed: 1996607doi: 10.1152/ajpcell.1991.260.2.c206google scholar: lookup
        5. Grounds MD. Age-associated changes in the response of skeletal muscle cells to exercise and regeneration.. Ann N Y Acad Sci 1998 Nov 20;854:78-91.
          pubmed: 9928422doi: 10.1111/j.1749-6632.1998.tb09894.xgoogle scholar: lookup
        6. Bischoff R. Enzymatic liberation of myogenic cells from adult rat muscle.. Anat Rec 1974 Dec;180(4):645-61.
          doi: 10.1002/ar.1091800410pubmed: 4374100google scholar: lookup
        7. Blau HM, Webster C. Isolation and characterization of human muscle cells.. Proc Natl Acad Sci U S A 1981 Sep;78(9):5623-7.
          doi: 10.1073/pnas.78.9.5623pmc: PMC348807pubmed: 6946499google scholar: lookup
        8. Yablonka-Reuveni Z, Quinn LS, Nameroff M. Isolation and clonal analysis of satellite cells from chicken pectoralis muscle.. Dev Biol 1987 Jan;119(1):252-9.
          doi: 10.1016/0012-1606(87)90226-0pmc: PMC4128172pubmed: 3025033google scholar: lookup
        9. McFarland DC, Doumit ME, Minshall RD. The turkey myogenic satellite cell: optimization of in vitro proliferation and differentiation.. Tissue Cell 1988;20(6):899-908.
          doi: 10.1016/0040-8166(88)90031-6pubmed: 3245037google scholar: lookup
        10. Greene EA, Raub RH. Procedures for Harvesting Satellite Cells from Equine Skeletal-Muscle. J Equine Vet Sci 1992;12(1):33–35.
          doi: 10.1016/S0737-0806(06)81383-3google scholar: lookup
        11. Dodson MV, Martin EL, Brannon MA, Mathison BA, McFarland DC. Optimization of bovine satellite cell-derived myotube formation in vitro.. Tissue Cell 1987;19(2):159-66.
          doi: 10.1016/0040-8166(87)90001-2pubmed: 3590147google scholar: lookup
        12. Dodson MV, McFarland DC, Martin EL, Brannon MA. Isolation of satellite cells from ovine skeletal muscles. Methods Cell Sci 1986;10(4):233–237.
        13. Doumit ME, Merkel RA. Conditions for isolation and culture of porcine myogenic satellite cells.. Tissue Cell 1992;24(2):253-62.
          doi: 10.1016/0040-8166(92)90098-Rpubmed: 1589873google scholar: lookup
        14. Kubis HP, Haller EA, Wetzel P, Gros G. Adult fast myosin pattern and Ca2+-induced slow myosin pattern in primary skeletal muscle culture.. Proc Natl Acad Sci U S A 1997 Apr 15;94(8):4205-10.
          doi: 10.1073/pnas.94.8.4205pmc: PMC20604pubmed: 9108130google scholar: lookup
        15. Huard J. Gene therapy and tissue engineering based on muscle derived stem cells: Potential for tissue regeneration. Abstr Paper Am Chem Soc 2004;227:U136–U136.
        16. Cerletti M, Jurga S, Witczak CA, Hirshman MF, Shadrach JL, Goodyear LJ, Wagers AJ. Highly efficient, functional engraftment of skeletal muscle stem cells in dystrophic muscles.. Cell 2008 Jul 11;134(1):37-47.
          doi: 10.1016/j.cell.2008.05.049pmc: PMC3665268pubmed: 18614009google scholar: lookup
        17. Deasy BM, Li Y, Huard J. Tissue engineering with muscle-derived stem cells.. Curr Opin Biotechnol 2004 Oct;15(5):419-23.
          doi: 10.1016/j.copbio.2004.08.004pubmed: 15464371google scholar: lookup
        18. Blau HM. Cell therapies for muscular dystrophy.. N Engl J Med 2008 Sep 25;359(13):1403-5.
          doi: 10.1056/NEJMcibr0805708pubmed: 18815403google scholar: lookup
        19. Edelman PD, McFarland DC, Mironov VA, Matheny JG. Commentary: In vitro-cultured meat production.. Tissue Eng 2005 May-Jun;11(5-6):659-62.
          pubmed: 15998207doi: 10.1089/ten.2005.11.659google scholar: lookup
        20. Datar I, Betti M. Possibilities for an in vitro meat production system. Innovat Food Sci Emerg Tech 2010;11(1):13–22.
          doi: 10.1016/j.ifset.2009.10.007google scholar: lookup
        21. Yablonka-Reuveni Z. Isolation and characterization of stem cells from adult skeletal muscle. Handbook of stem cells Elsevier-Academic Press, San Diego; 2004; pp. 571–580.
        22. Seale P, Sabourin LA, Girgis-Gabardo A, Mansouri A, Gruss P, Rudnicki MA. Pax7 is required for the specification of myogenic satellite cells.. Cell 2000 Sep 15;102(6):777-86.
          doi: 10.1016/S0092-8674(00)00066-0pubmed: 11030621google scholar: lookup
        23. Holzer N, Hogendoorn S, Zürcher L, Garavaglia G, Yang S, König S, Laumonier T, Menetrey J. Autologous transplantation of porcine myogenic precursor cells in skeletal muscle.. Neuromuscul Disord 2005 Mar;15(3):237-44.
          doi: 10.1016/j.nmd.2004.11.001pubmed: 15725585google scholar: lookup
        24. Wilschut KJ, Haagsman HP, Roelen BA. Extracellular matrix components direct porcine muscle stem cell behavior.. Exp Cell Res 2010 Feb 1;316(3):341-52.
          doi: 10.1016/j.yexcr.2009.10.014pubmed: 19853598google scholar: lookup
        25. Fernandez MS, Dennis JE, Drushel RF, Carrino DA, Kimata K, Yamagata M, Caplan AI. The dynamics of compartmentalization of embryonic muscle by extracellular matrix molecules.. Dev Biol 1991 Sep;147(1):46-61.
          doi: 10.1016/S0012-1606(05)80006-5pubmed: 1879615google scholar: lookup
        26. Bullaro JC, Brookman DH. Comparison of skeletal muscle monolayer cultures initiated with cells dissociated by the vortex and trypsin methods.. In Vitro 1976 Aug;12(8):564-70.
          pubmed: 1033143doi: 10.1007/bf02797440google scholar: lookup
        27. Caplan AI. A simplified procedure for preparing myogenic cells for culture.. J Embryol Exp Morphol 1976 Aug;36(1):175-81.
          pubmed: 789809
        28. O'Neill MC, Stockdale FE. A kinetic analysis of myogenesis in vitro.. J Cell Biol 1972 Jan;52(1):52-65.
          doi: 10.1083/jcb.52.1.52pmc: PMC2108680pubmed: 5006948google scholar: lookup
        29. Woods TL, Smith CW, Zeece MG, Jones SJ. Conditions for the culture of bovine embryonic myogenic cells.. Tissue Cell 1997 Apr;29(2):207-15.
          doi: 10.1016/S0040-8166(97)80020-1pubmed: 9149443google scholar: lookup
        30. Benders AA, van Kuppevelt TH, Oosterhof A, Veerkamp JH. The biochemical and structural maturation of human skeletal muscle cells in culture: the effect of the serum substitute Ultroser G.. Exp Cell Res 1991 Aug;195(2):284-94.
          doi: 10.1016/0014-4827(91)90375-5pubmed: 1649054google scholar: lookup
        31. Halevy O, Piestun Y, Allouh MZ, Rosser BW, Rinkevich Y, Reshef R, Rozenboim I, Wleklinski-Lee M, Yablonka-Reuveni Z. Pattern of Pax7 expression during myogenesis in the posthatch chicken establishes a model for satellite cell differentiation and renewal.. Dev Dyn 2004 Nov;231(3):489-502.
          doi: 10.1002/dvdy.20151pubmed: 15390217google scholar: lookup
        32. Barani AE, Sabido O, Freyssenet D. Mitotic activity of rat muscle satellite cells in response to serum stimulation: relation with cellular metabolism.. Exp Cell Res 2003 Feb 15;283(2):196-205.
          doi: 10.1016/S0014-4827(02)00030-7pubmed: 12581739google scholar: lookup
        33. Alexander LS, Mahajan A, Odle J, Flann KL, Rhoads RP, Stahl CH. Dietary phosphate restriction decreases stem cell proliferation and subsequent growth potential in neonatal pigs.. J Nutr 2010 Mar;140(3):477-82.
          doi: 10.3945/jn.109.117390pubmed: 20053936google scholar: lookup
        34. Yablonka-Reuveni Z, Nameroff M. Temporal differences in desmin expression between myoblasts from embryonic and adult chicken skeletal muscle.. Differentiation 1990 Oct;45(1):21-8.
          doi: 10.1111/j.1432-0436.1990.tb00452.xpmc: PMC4038325pubmed: 2292359google scholar: lookup
        35. Dodson MV, McFarland DC, Grant AL, Doumit ME, Velleman SG. Extrinsic regulation of domestic animal-derived satellite cells.. Domest Anim Endocrinol 1996 Mar;13(2):107-26.
          doi: 10.1016/0739-7240(95)00062-3pubmed: 8665800google scholar: lookup
        36. Soeta C, Yamanouchi K, Hasegawa T, Ishida N, Mukoyama H, Tojo H, Tachi C. Isolation of Satellite Cells from Equine Skeletal Muscle. J Equine Sci 1998;9(3):97–100.
          doi: 10.1294/jes.9.97google scholar: lookup
        37. Byrne KM, Vierck J, Dodson MV. In vitro model of equine muscle regeneration.. Equine Vet J 2000 Sep;32(5):401-5.
          pubmed: 11037261doi: 10.2746/042516400777591020google scholar: lookup
        38. Muroya S, Nakajima I, Chikuni K. Bovine skeletal muscle cells predominantly express a vascular cell adhesion molecule-1 seven-Ig domain splice form. Zoolog Sci 2001;18(6):797–805.
          doi: 10.2108/zsj.18.797google scholar: lookup
        39. Cassar-Malek I, Langlois N, Picard B, Geay Y. Regulation of bovine satellite cell proliferation and differentiation by insulin and triiodothyronine.. Domest Anim Endocrinol 1999 Nov;17(4):373-88.
          doi: 10.1016/S0739-7240(99)00055-7pubmed: 10628428google scholar: lookup
        40. Allen RE, Rankin LL, Greene EA, Boxhorn LK, Johnson SE, Taylor RG, Pierce PR. Desmin is present in proliferating rat muscle satellite cells but not in bovine muscle satellite cells.. J Cell Physiol 1991 Dec;149(3):525-35.
          doi: 10.1002/jcp.1041490323pubmed: 1744177google scholar: lookup
        41. Sultan KR, Henkel B, Terlou M, Haagsman HP. Quantification of hormone-induced atrophy of large myotubes from C2C12 and L6 cells: atrophy-inducible and atrophy-resistant C2C12 myotubes.. Am J Physiol Cell Physiol 2006 Feb;290(2):C650-9.
          pubmed: 16176969doi: 10.1152/ajpcell.00163.2005google scholar: lookup
        42. Blanton JR Jr, Grant AL, McFarland DC, Robinson JP, Bidwell CA. Isolation of two populations of myoblasts from porcine skeletal muscle.. Muscle Nerve 1999 Jan;22(1):43-50.
          doi: 10.1002/(SICI)1097-4598(199901)22:1<43::AID-MUS8>3.0.CO;2-Opubmed: 9883856google scholar: lookup
        43. Snow MH. The effects of aging on satellite cells in skeletal muscles of mice and rats.. Cell Tissue Res 1977 Dec 19;185(3):399-408.
          pubmed: 597854doi: 10.1007/bf00220299google scholar: lookup
        44. Brack AS, Bildsoe H, Hughes SM. Evidence that satellite cell decrement contributes to preferential decline in nuclear number from large fibres during murine age-related muscle atrophy.. J Cell Sci 2005 Oct 15;118(Pt 20):4813-21.
          doi: 10.1242/jcs.02602pubmed: 16219688google scholar: lookup
        45. Shefer G, Van de Mark DP, Richardson JB, Yablonka-Reuveni Z. Satellite-cell pool size does matter: defining the myogenic potency of aging skeletal muscle.. Dev Biol 2006 Jun 1;294(1):50-66.
          doi: 10.1016/j.ydbio.2006.02.022pmc: PMC2710453pubmed: 16554047google scholar: lookup
        46. Gibson MC, Schultz E. Age-related differences in absolute numbers of skeletal muscle satellite cells.. Muscle Nerve 1983 Oct;6(8):574-80.
          doi: 10.1002/mus.880060807pubmed: 6646160google scholar: lookup
        47. Carlson BM, Faulkner JA. Muscle transplantation between young and old rats: age of host determines recovery.. Am J Physiol 1989 Jun;256(6 Pt 1):C1262-6.
          pubmed: 2735398doi: 10.1152/ajpcell.1989.256.6.c1262google scholar: lookup
        48. Schultz E, Lipton BH. Skeletal muscle satellite cells: changes in proliferation potential as a function of age.. Mech Ageing Dev 1982 Dec;20(4):377-83.
          doi: 10.1016/0047-6374(82)90105-1pubmed: 7166986google scholar: lookup
        49. Renault V, Piron-Hamelin G, Forestier C, DiDonna S, Decary S, Hentati F, Saillant G, Butler-Browne GS, Mouly V. Skeletal muscle regeneration and the mitotic clock.. Exp Gerontol 2000 Sep;35(6-7):711-9.
          pubmed: 11053661doi: 10.1016/s0531-5565(00)00151-0google scholar: lookup
        50. Shavlakadze T, McGeachie J, Grounds MD. Delayed but excellent myogenic stem cell response of regenerating geriatric skeletal muscles in mice.. Biogerontology 2010 Jun;11(3):363-76.
          doi: 10.1007/s10522-009-9260-0pubmed: 20033288google scholar: lookup
        51. Allen RE, Temm-Grove CJ, Sheehan SM, Rice G. Skeletal muscle satellite cell cultures.. Methods Cell Biol 1997;52:155-76.
          pubmed: 9379949doi: 10.1016/s0091-679x(08)60378-7google scholar: lookup

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          doi: 10.1016/j.bbrep.2022.101391pubmed: 36504704google scholar: lookup
        2. Kim SH, Kim CJ, Lee EY, Son YM, Hwang YH, Joo ST. Optimal Pre-Plating Method of Chicken Satellite Cells for Cultured Meat Production.. Food Sci Anim Resour 2022 Nov;42(6):942-952.
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          doi: 10.1038/s41538-022-00157-zpubmed: 36175443google scholar: lookup
        4. Knežić T, Janjušević L, Djisalov M, Yodmuang S, Gadjanski I. Using Vertebrate Stem and Progenitor Cells for Cellular Agriculture, State-of-the-Art, Challenges, and Future Perspectives.. Biomolecules 2022 May 13;12(5).
          doi: 10.3390/biom12050699pubmed: 35625626google scholar: lookup
        5. Pajčin I, Knežić T, Savic Azoulay I, Vlajkov V, Djisalov M, Janjušević L, Grahovac J, Gadjanski I. Bioengineering Outlook on Cultivated Meat Production.. Micromachines (Basel) 2022 Feb 28;13(3).
          doi: 10.3390/mi13030402pubmed: 35334693google scholar: lookup
        6. Skrivergaard S, Rasmussen MK, Therkildsen M, Young JF. Bovine Satellite Cells Isolated after 2 and 5 Days of Tissue Storage Maintain the Proliferative and Myogenic Capacity Needed for Cultured Meat Production.. Int J Mol Sci 2021 Aug 4;22(16).
          doi: 10.3390/ijms22168376pubmed: 34445082google scholar: lookup
        7. Wang H, He K, Zeng X, Zhou X, Yan F, Yang S, Zhao A. Isolation and identification of goose skeletal muscle satellite cells and preliminary study on the function of C1q and tumor necrosis factor-related protein 3 gene.. Anim Biosci 2021 Jun;34(6):1078-1087.
          doi: 10.5713/ajas.20.0430pubmed: 33152229google scholar: lookup
        8. Carrero-Rojas G, Benítez-Temiño B, Pastor AM, Davis López de Carrizosa MA. Muscle Progenitors Derived from Extraocular Muscles Express Higher Levels of Neurotrophins and their Receptors than other Cranial and Limb Muscles.. Cells 2020 Mar 18;9(3).
          doi: 10.3390/cells9030747pubmed: 32197508google scholar: lookup
        9. Metzger K, Tuchscherer A, Palin MF, Ponsuksili S, Kalbe C. Establishment and validation of cell pools using primary muscle cells derived from satellite cells of pig skeletal muscle.. In Vitro Cell Dev Biol Anim 2020 Mar;56(3):193-199.
          doi: 10.1007/s11626-019-00428-2pubmed: 31873830google scholar: lookup
        10. Najjar SA, Smith AST, Long CJ, McAleer CW, Cai Y, Srinivasan B, Martin C, Vandenburgh HH, Hickman JJ. A multiplexed in vitro assay system for evaluating human skeletal muscle functionality in response to drug treatment.. Biotechnol Bioeng 2020 Mar;117(3):736-747.
          doi: 10.1002/bit.27231pubmed: 31758543google scholar: lookup
        11. Wang S, Sun Y, Ren R, Xie J, Tian X, Zhao S, Li X, Cao J. H3K27me3 Depletion during Differentiation Promotes Myogenic Transcription in Porcine Satellite Cells.. Genes (Basel) 2019 Mar 19;10(3).
          doi: 10.3390/genes10030231pubmed: 30893875google scholar: lookup
        12. Amilon KR, Cortes-Araya Y, Moore B, Lee S, Lillico S, Breton A, Esteves CL, Donadeu FX. Generation of Functional Myocytes from Equine Induced Pluripotent Stem Cells.. Cell Reprogram 2018 Oct;20(5):275-281.
          doi: 10.1089/cell.2018.0023pubmed: 30207795google scholar: lookup
        13. Miersch C, Stange K, Röntgen M. Effects of trypsinization and of a combined trypsin, collagenase, and DNase digestion on liberation and in vitro function of satellite cells isolated from juvenile porcine muscles.. In Vitro Cell Dev Biol Anim 2018 Jun;54(6):406-412.
          doi: 10.1007/s11626-018-0263-5pubmed: 29785535google scholar: lookup
        14. Miersch C, Stange K, Röntgen M. Separation of functionally divergent muscle precursor cell populations from porcine juvenile muscles by discontinuous Percoll density gradient centrifugation.. BMC Cell Biol 2018 Mar 9;19(1):2.
          doi: 10.1186/s12860-018-0156-1pubmed: 29523096google scholar: lookup
        15. Tong HL, Jiang RY, Zhang WW, Yan YQ. MiR-2425-5p targets RAD9A and MYOG to regulate the proliferation and differentiation of bovine skeletal muscle-derived satellite cells.. Sci Rep 2017 Mar 24;7(1):418.
          doi: 10.1038/s41598-017-00470-8pubmed: 28341832google scholar: lookup
        16. Sebastian S, Goulding L, Kuchipudi SV, Chang KC. Extended 2D myotube culture recapitulates postnatal fibre type plasticity.. BMC Cell Biol 2015 Sep 17;16:23.
          doi: 10.1186/s12860-015-0069-1pubmed: 26382633google scholar: lookup
        17. Will K, Schering L, Albrecht E, Kalbe C, Maak S. Differentiation of bovine satellite cell-derived myoblasts under different culture conditions.. In Vitro Cell Dev Biol Anim 2015 Oct;51(9):885-9.
          doi: 10.1007/s11626-015-9916-9pubmed: 26091626google scholar: lookup
        18. Baquero-Perez B, Kuchipudi SV, Ho J, Sebastian S, Puranik A, Howard W, Brookes SM, Brown IH, Chang KC. Chicken and duck myotubes are highly susceptible and permissive to influenza virus infection.. J Virol 2015 Mar;89(5):2494-506.
          doi: 10.1128/JVI.03421-14pubmed: 25540384google scholar: lookup
        19. Froehlich JM, Seiliez I, Gabillard JC, Biga PR. Preparation of primary myogenic precursor cell/myoblast cultures from basal vertebrate lineages.. J Vis Exp 2014 Apr 30;(86).
          doi: 10.3791/51354pubmed: 24835774google scholar: lookup
        20. Patel AK, Tripathi AK, Patel UA, Shah RK, Joshi CG. Myostatin knockdown and its effect on myogenic gene expression program in stably transfected goat myoblasts.. In Vitro Cell Dev Biol Anim 2014 Aug;50(7):587-96.
          doi: 10.1007/s11626-014-9743-4pubmed: 24682647google scholar: lookup
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