FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / Immunogenetics / A soluble class I molecule analogous to mouse Q10 in the hor...
Immunogenetics1986; 23(5); 277-283; doi: 10.1007/BF00398789

A soluble class I molecule analogous to mouse Q10 in the horse and related species.

Abstract: Horse serum is shown to contain a soluble class I molecule analogous to the secreted Q10 molecule in the mouse. This molecule has several similarities to the recently described mouse Q10 molecule: it is smaller than membrane-bound equine class I molecules; it occurs in a high molecular mass complex of 200-300 kd in serum; and the serum levels of the equine molecule are similar to that of the Q10 molecule (about 30 micrograms/ml). A soluble molecule is also detected in the sera of species related to the horse; it has in fact been found in all the wild members of the order Perissodactyla so far tested. However, it was not detected in the serum of members of the orders Carnivora, Sirenia, Proboscidea, Artiodactyla, and Primates that were tested, nor in the serum of members of the order Rodentia other than in that of the genus Mus.
Get Full Text
Publication Date: 1986-01-01 PubMed ID: 3519445DOI: 10.1007/BF00398789Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Comparative Study
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research study reveals the presence of a soluble class I molecule, similar to the mouse Q10 molecule, in horse serum and in various other species related to the horse.

Overview of the research

  • The study primarily focuses on identifying the presence of a soluble class I molecule, similar to the secreted Q10 molecule found in mice, in the serum of horses and other related species. The Q10 molecule in mice is unique due to its soluble nature, which differs from most class I molecules that are typically membrane-bound.
  • Horse serum was tested to find this particular type of molecule, and the results revealed a molecule with several similarities to the Q10 molecule in mice. Specifically, the molecule in horse serum was smaller than the usual equine class I molecules and was found to be part of a high molecular mass complex of 200-300 kd in the serum.
  • Further, the concentration levels of this molecule in horse were comparable to that of Q10 (around 30 micrograms/ml) demonstrating a similarity in their presence.

Testing the Presence in Other Species

  • Following the identification of this molecule in horse serum, the researchers expanded their study to various species related to the horse.
  • In every wild member of the order Perissodactyla tested, a soluble molecule, similar to the one found in horse serum, was identified.
  • Interestingly, this soluble molecule was not detected in members of several other orders, including Carnivora, Sirenia, Proboscidea, Artiodactyla, and Primates. Likewise, this molecule was not found in the serum of the order Rodentia, with the exception of the genus Mus, which marks a significant distinction.

Significance of Research

  • This research contributes to our understanding of soluble class I molecules. Not only does it identify the presence of such a soluble molecule in horse serum and related species, but it also reveals its absence in various other orders.
  • Finding the equivalent of the mouse Q10 molecule in the studied species opens up potential new paths for studying molecular biology and the evolving adaptation mechanisms at the genetic level within these species.

Cite This Article

APA
Lew AM, Valas RB, Maloy WL, Coligan JE. (1986). A soluble class I molecule analogous to mouse Q10 in the horse and related species. Immunogenetics, 23(5), 277-283. https://doi.org/10.1007/BF00398789

Publication

Immunogenetics
ISSN: 0093-7711
NlmUniqueID: 0420404
Country: United States
Language: English
Volume: 23
Issue: 5
Pages: 277-283

Researcher Affiliations

Lew, A M
    Valas, R B
      Maloy, W L
        Coligan, J E

          MeSH Terms

          • Animals
          • Biological Evolution
          • Histocompatibility Antigens / analysis
          • Horses / immunology
          • Macromolecular Substances
          • Mice
          • Molecular Weight
          • Perissodactyla / genetics
          • Perissodactyla / immunology
          • Solubility
          • beta 2-Microglobulin / metabolism

          References

          This article includes 19 references
          1. Siwarski DF, Barra Y, Jay G, Rogers MJ. Occurrence of a unique MHC class I gene in distantly related members of the genus Mus.. Immunogenetics 1985;21(3):267-76.
            pubmed: 3988322doi: 10.1007/BF00375379google scholar: lookup
          2. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.. Nature 1970 Aug 15;227(5259):680-5.
            pubmed: 5432063doi: 10.1038/227680a0google scholar: lookup
          3. Maloy WL, Coligan JE, Barra Y, Jay G. Detection of a secreted form of the murine H-2 class I antigen with an antibody against its predicted carboxyl terminus.. Proc Natl Acad Sci U S A 1984 Feb;81(4):1216-20.
            pubmed: 6583704doi: 10.1073/pnas.81.4.1216google scholar: lookup
          4. Pellegrino MA, Russo C, Allison JP. HLA antigens in serum.. Methods Enzymol 1984;108:614-24.
            pubmed: 6527659doi: 10.1016/s0076-6879(84)08122-2google scholar: lookup
          5. Winoto A, Steinmetz M, Hood L. Genetic mapping in the major histocompatibility complex by restriction enzyme site polymorphisms: most mouse class I genes map to the Tla complex.. Proc Natl Acad Sci U S A 1983 Jun;80(11):3425-9.
            pubmed: 6304710doi: 10.1073/pnas.80.11.3425google scholar: lookup
          6. Lew AM, Hosking CS, Studdert MJ. Immunologic aspects of combined immunodeficiency disease in Arabian foals.. Am J Vet Res 1980 Aug;41(8):1161-6.
            pubmed: 7004276
          7. Cosman D, Kress M, Khoury G, Jay G. Tissue-specific expression of an unusual H-2 (class I)-related gene.. Proc Natl Acad Sci U S A 1982 Aug;79(16):4947-51.
            pubmed: 6956903doi: 10.1073/pnas.79.16.4947google scholar: lookup
          8. Lögdberg L, Cigén R, Björck L. Binding of beta 2-microglobulin by heterologous sera.. Scand J Immunol 1981;13(3):237-43.
            pubmed: 6165075doi: 10.1111/j.1365-3083.1981.tb00131.xgoogle scholar: lookup
          9. Mellor AL, Weiss EH, Kress M, Jay G, Flavell RA. A nonpolymorphic class I gene in the murine major histocompatibility complex.. Cell 1984 Jan;36(1):139-44.
            pubmed: 6420070doi: 10.1016/0092-8674(84)90082-5google scholar: lookup
          10. Lalanne JL, Transy C, Guerin S, Darche S, Meulien P, Kourilsky P. Expression of class I genes in the major histocompatibility complex: identification of eight distinct mRNAs in DBA/2 mouse liver.. Cell 1985 Jun;41(2):469-78.
            pubmed: 3838699doi: 10.1016/s0092-8674(85)80020-9google scholar: lookup
          11. Lew AM, Maloy WL, Coligan JE. Characteristics of the expression of the murine soluble class I molecule (Q10).. J Immunol 1986 Jan;136(1):254-8.
            pubmed: 3940270
          12. Rogers MJ. An improved preparation of murine histocompatibility antigens (H-2b) and a novel membrane binding form of H-2Kb.. Biochem Biophys Res Commun 1981 Jul 30;101(2):426-33.
            pubmed: 7306086doi: 10.1016/0006-291x(81)91277-8google scholar: lookup
          13. Ploegh HL, Cannon LE, Strominger JL. Cell-free translation of the mRNAs for the heavy and light chains of HLA-A and HLA-B antigens.. Proc Natl Acad Sci U S A 1979 May;76(5):2273-7.
            pubmed: 287067doi: 10.1073/pnas.76.5.2273google scholar: lookup
          14. Kress M, Cosman D, Khoury G, Jay G. Secretion of a transplantation-related antigen.. Cell 1983 Aug;34(1):189-96.
            pubmed: 6411351doi: 10.1016/0092-8674(83)90149-6google scholar: lookup
          15. Evrin PE, Peterson PA, Wide L, Berggård I. Radioimmunoassay of 2 -microglobulin in human biological fluids.. Scand J Clin Lab Invest 1971 Dec;28(4):439-43.
            pubmed: 4110090doi: 10.3109/00365517109095721google scholar: lookup
          16. Devlin JJ, Lew AM, Flavell RA, Coligan JE. Secretion of a soluble class I molecule encoded by the Q10 gene of the C57BL/10 mouse.. EMBO J 1985 Feb;4(2):369-74.
            pubmed: 4018029doi: 10.1002/j.1460-2075.1985.tb03638.xgoogle scholar: lookup
          17. Flaherty L, DiBiase K, Lynes MA, Seidman JG, Weinberger O, Rinchik EM. Characterization of a Q subregion gene in the murine major histocompatibility complex.. Proc Natl Acad Sci U S A 1985 Mar;82(5):1503-7.
            pubmed: 2983348doi: 10.1073/pnas.82.5.1503google scholar: lookup
          18. Kvist S, Klein J, Peterson PA. Genetic control of the serum concentration of an H-2-antigen-like polypeptide chain.. Immunogenetics 1980;10(5):499-507.
            pubmed: 22457922doi: 10.1007/BF01572584google scholar: lookup
          19. Weiss EH, Golden L, Fahrner K, Mellor AL, Devlin JJ, Bullman H, Tiddens H, Bud H, Flavell RA. Organization and evolution of the class I gene family in the major histocompatibility complex of the C57BL/10 mouse.. Nature 1984 Aug 23-29;310(5979):650-5.
            pubmed: 6088985doi: 10.1038/310650a0google scholar: lookup

          Citations

          This article has been cited 7 times.
          1. Pamir N, Pan C, Plubell DL, Hutchins PM, Tang C, Wimberger J, Irwin A, Vallim TQA, Heinecke JW, Lusis AJ. Genetic control of the mouse HDL proteome defines HDL traits, function, and heterogeneity. J Lipid Res 2019 Mar;60(3):594-608.
            doi: 10.1194/jlr.M090555pubmed: 30622162google scholar: lookup
          2. Bailey E. Segregation distortion within the equine MHC; analogy to a mouse T/t-complex trait. Immunogenetics 1986;24(4):225-9.
            doi: 10.1007/BF00364526pubmed: 3781570google scholar: lookup
          3. Lew AM, Bailey E, Valas RB, Coligan J. The gene encoding the equine soluble class I molecule is linked to the horse MHC. Immunogenetics 1986;24(2):128-30.
            doi: 10.1007/BF00373122pubmed: 3744437google scholar: lookup
          4. Spencer SC, Fabre JW. Identification in rat liver and serum of water-soluble class I MHC molecules possibly homologous to the murine Q10 gene product. J Exp Med 1987 Jun 1;165(6):1595-608.
            doi: 10.1084/jem.165.6.1595pubmed: 3585249google scholar: lookup
          5. Dobbe LM, Stam NJ, Neefjes JJ, Giphart MJ. Biochemical complexity of serum HLA class I molecules. Immunogenetics 1988;27(3):203-10.
            doi: 10.1007/BF00346587pubmed: 3276619google scholar: lookup
          6. Margulies DH, Lopez R, Boyd LF, McCluskey J. Engineering soluble major histocompatibility molecules: why and how. Immunol Res 1987;6(1-2):101-16.
            doi: 10.1007/BF02918107pubmed: 3114395google scholar: lookup
          7. Lew AM, McCluskey J, Maloy WL, Margulies DH, Coligan JE. Multiple class I molecules generated from single genes by alternative splicing of pre-mRNAs. Immunol Res 1987;6(1-2):117-32.
            doi: 10.1007/BF02918108pubmed: 2442272google scholar: lookup
          Subscribe to our newsletter
          Join 99,105 horse owners like you who receive our email updates on horse health and nutrition.