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Home / Equine Research Bank / J Equine Sci / A Study on the Presence of Ferritin-binding Proteins in Feta...
Journal of equine science2011; 22(1); 1-7; doi: 10.1294/jes.22.1

A Study on the Presence of Ferritin-binding Proteins in Fetal Horse Plasma.

Abstract: In mammal circulation, ferritin-binding proteins (FBPs) are thought to be involved in clearance of circulating ferritin after complex formation with it through receptor-mediated uptake. However, there is no report on fetal FBP in fetal circulation. Although iron concentrations of fetal horse plasma were higher than those of adult horse plasma, plasma ferritin concentrations and ferritin-binding activities were found to be significantly lower in fetus than in adult. FBPs were purified from fetal or adult horse plasma on horse spleen ferritin-Sepharose 4B affinity column. Partially affinity-purified fetal horse plasma FBPs were mainly separated into 65 and 41 kDa bands in addition to minor bands with higher molecular masses ranged from 102 to 140 kDa on SDS-PAGE under reducing condition. The adult horse plasma FBPs were separated into 74, 54 and 28 kDa bands, and the 74 and 54 kDa bands reacted with antibodies specific for horse IgM and IgG heavy chains, respectively, by immunoblotting analyses. On the other hand, no antibodies to horse immunoglobulin classes detected any bands in fetal horse plasma FBPs. The affinity-purified adult and fetal horse plasma FBPs did not contain fibrinogen as a plasma specific FBP, probably due to its lower affinity to the ligand ferritin. These results demonstrate the presence of FBPs which are different from adult horse plasma FBPs including anti-ferritin autoantibodies in fetal plasma.
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Publication Date: 2011-04-26 PubMed ID: 24833981PubMed Central: PMC4013997DOI: 10.1294/jes.22.1Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research study investigates the presence of ferritin-binding proteins (FBPs) in the blood plasma of fetal horses. The study discovered FBPs differing from those found in adult horses, contributing to the understanding of iron regulation in prenatal equine health.

Objective of the Study

  • The research was conducted with the primary aim to investigate whether ferritin-binding proteins (FBPs), crucial for regulating iron in the bloodstream, exist in the fetal horse plasma.

Main Findings

  • Comparative analysis revealed that while iron concentrations in fetal horse plasma exceeded those of adult horses, plasma ferritin levels and ferritin-binding activities were significantly lower in fetuses.
  • FBPs in fetal and adult horse plasma were purified for detailed analysis. The purified fetal FBPs showed two main bands at 65 and 41 kDa, with additional minor bands ranging from 102 to 140 kDa.
  • Adult horse plasma FBPs exhibited bands at 74, 54, and 28 kDa. The bands at 74 and 54 kDa reacted to antibodies specific for horse IgM and IgG heavy chains, suggested to be tied to immunoglobulin (the body’s immune response agent).
  • However, no such reaction was found in the fetal horse plasma FBPs, indicating the absence of these specific immunoglobulins.

Significance of the Study

  • The absence of fibrinogen (a plasma-specific FBP) in both adult and fetal FBPs suggests a lower affinity for the ligand ferritin, raising questions about its role in iron regulation in horses.
  • The presence of different FBPs in fetal plasma, especially those not reacting to specific antibodies, implies a unique anti-ferritin autoantibodies mechanism in fetal horses.
  • The study opens the door for further research into iron regulation mechanisms in prenatal equine health, potentially leading to improvement in foal health and survival rates.

Cite This Article

APA
Hashimoto M, Nambo Y, Kondo T, Watanabe K, Orino K. (2011). A Study on the Presence of Ferritin-binding Proteins in Fetal Horse Plasma. J Equine Sci, 22(1), 1-7. https://doi.org/10.1294/jes.22.1

Publication

Journal of equine science
ISSN: 1340-3516
NlmUniqueID: 9503751
Country: Japan
Language: English
Volume: 22
Issue: 1
Pages: 1-7

Researcher Affiliations

Hashimoto, Masafumi
  • Laboratory of Veterinary Biochemistry, School of Veterinary Medicine, Kitasato University, Aomori 034-8628, Japan.
Nambo, Yasuo
  • Equine Science Division, Hidaka Training Research Center, Japan Racing Association, 535-13 Nishicha, Urakawa-cho, Urakawa-gun, Hidaka, Hokkaido 057-0171, Japan.
Kondo, Takashi
  • Epizootic Research Center, Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke-shi, Tochigi 329-0412, Japan.
Watanabe, Kiyotaka
  • Laboratory of Veterinary Biochemistry, School of Veterinary Medicine, Kitasato University, Aomori 034-8628, Japan.
Orino, Koichi
  • Laboratory of Veterinary Biochemistry, School of Veterinary Medicine, Kitasato University, Aomori 034-8628, Japan.

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Citations

This article has been cited 1 times.
  1. Russell EB, Courtman NF, Santos LL, Tennent-Brown BS. Fibrinogen heterogeneity in horses.. J Vet Intern Med 2021 Mar;35(2):1131-1139.
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