A viable foal obtained by equine somatic cell nuclear transfer using oocytes recovered from immature follicles of live mares.
Abstract: The presence of heterogenous mitochondria from the host ooplast affects the acceptance of offspring obtained by somatic cell nuclear transfer. This might be avoided by obtaining oocytes from selected females, but is then complicated by low numbers of available oocytes. We examined the efficiency of equine somatic cell nuclear transfer using oocytes recovered by transvaginal aspiration of immature follicles from 11 mares. Use of metaphase I oocytes as cytoplasts and of scriptaid (a histone deacetylase inhibitor) treatment during oocyte activation were evaluated to determine if these approaches would increase blastocyst production. In experiment 1, blastocyst development was 0/14 for metaphase I oocytes and 4/103 (4%) for metaphase II oocytes. Three blastocysts were transferred to recipient mares, resulting in two pregnancies and one live foal, which died shortly after birth. In experiment 2, blastocyst development was 2/47 (4%) for control oocytes and 1/83 (1%) for scriptaid-treated oocytes. No foals were born from two blastocysts transferred in the control group. The blastocyst from the scriptaid treatment resulted in birth of a live foal. In conclusion, this is apparently the first report of production of a viable cloned foal from oocytes collected from immature follicles of live mares, supporting the possibility of cloning using oocytes from selected mares.
Copyright © 2013 Elsevier Inc. All rights reserved.
Publication Date: 2013-01-11 PubMed ID: 23312717DOI: 10.1016/j.theriogenology.2012.12.005Google Scholar: Lookup
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- Clinical Trial
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article discusses the potential of using oocytes collected from immature follicles of live mares in equine somatic cell nuclear transfer, showing the successful production of a viable foal from this method, indicating possibilities for cloning from selected mares.
Understanding the Study
- The research seeks to solve the problem of heterogenous mitochondria from the host ooplast affecting the acceptance of offspring obtained by somatic cell nuclear transfer, a process often used in the cloning of animals. The presence of different types of mitochondria from the host can lead to complications in the development and survival of the cloned offspring.
- In general, obtaining oocytes from selected females can avoid this problem. However, the challenge here is that the number of available oocytes for collection can be quite low.
- To try to alleviate this issue, the researchers used oocytes recovered from the immature follicles of 11 live mares. This approach is novel as immature follicles are traditionally seen as non-ideal for such procedures due to their incomplete development.
Methodology and Findings
- Two different approaches were explored in the study: the use of metaphase I oocytes as cytoplasts and the use of scriptaid (a type of histone deacetylase inhibitor) during oocyte activation.
- The efficiency of these methods was evaluated by observing whether they increase blastocyst production, which is a critical stage in the development of an embryo.
- The results showed that metaphase I oocytes did not yield any blastocyst development. However, metaphase II oocytes produced a 4% success rate. This resulted in two pregnancies and the birth of one live foal, which unfortunately died shortly after birth.
- With scriptaid treatment, the success rate was lower at 1%, but it did result in the birth of a live foal.
Conclusions and Implications
- This study offered the first report of a viable cloned foal produced from oocytes collected from immature follicles of live mares.
- This finding is significant as it supports the possibility of using cloning techniques with oocytes from selected mares, potentially providing a compelling solution to the challenges of cloning livestock.
- However, the overall success rates were low, indicating that further research and optimization are required for this method to be practically usable on a large scale.
Cite This Article
APA
Choi YH, Norris JD, Velez IC, Jacobson CC, Hartman DL, Hinrichs K.
(2013).
A viable foal obtained by equine somatic cell nuclear transfer using oocytes recovered from immature follicles of live mares.
Theriogenology, 79(5), 791-6.e1.
https://doi.org/10.1016/j.theriogenology.2012.12.005 Publication
Researcher Affiliations
- College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, Texas, USA.
MeSH Terms
- Animals
- Cloning, Organism / methods
- Cloning, Organism / veterinary
- Embryo Transfer / veterinary
- Embryonic Development
- Female
- Horses / physiology
- Nuclear Transfer Techniques / veterinary
- Pregnancy
- Pregnancy Rate
Citations
This article has been cited 6 times.- Salamone D, Maserati M. Horse Somatic Cell Nuclear Transfer Using Zona Pellucida-Enclosed and Zona-Free Oocytes.. Methods Mol Biol 2023;2647:269-281.
- Hisey EA, Ross PJ, Meyers S. Genetic Manipulation of the Equine Oocyte and Embryo.. J Equine Vet Sci 2021 Apr;99:103394.
- Olivera R, Moro LN, Jordan R, Pallarols N, Guglielminetti A, Luzzani C, Miriuka SG, Vichera G. Bone marrow mesenchymal stem cells as nuclear donors improve viability and health of cloned horses.. Stem Cells Cloning 2018;11:13-22.
- Olivera R, Moro LN, Jordan R, Luzzani C, Miriuka S, Radrizzani M, Donadeu FX, Vichera G. In Vitro and In Vivo Development of Horse Cloned Embryos Generated with iPSCs, Mesenchymal Stromal Cells and Fetal or Adult Fibroblasts as Nuclear Donors.. PLoS One 2016;11(10):e0164049.
- Gambini A, De Stefano A, Bevacqua RJ, Karlanian F, Salamone DF. The aggregation of four reconstructed zygotes is the limit to improve the developmental competence of cloned equine embryos.. PLoS One 2014;9(11):e110998.
- Martino NA, Dell'Aquila ME, Filioli Uranio M, Rutigliano L, Nicassio M, Lacalandra GM, Hinrichs K. Effect of holding equine oocytes in meiosis inhibitor-free medium before in vitro maturation and of holding temperature on meiotic suppression and mitochondrial energy/redox potential.. Reprod Biol Endocrinol 2014 Oct 11;12:99.
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