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Home / Equine Research Bank / J Clin Microbiol / Ability of the vector tick Boophilus microplus to acquire an...
Journal of clinical microbiology2005; 43(8); 3755-3759; doi: 10.1128/JCM.43.8.3755-3759.2005

Ability of the vector tick Boophilus microplus to acquire and transmit Babesia equi following feeding on chronically infected horses with low-level parasitemia.

Abstract: The protozoan parasite Babesia equi replicates within erythrocytes. During the acute phase of infection, B. equi can reach high levels of parasitemia, resulting in a hemolytic crisis. Horses that recover from the acute phase of the disease remain chronically infected. Subsequent transmission is dependent upon the ability of vector ticks to acquire B. equi and, following development and replication, establishment of B. equi in the salivary glands. Although restriction of the movement of chronically infected horses with B. equi is based on the presumption that ticks can acquire and transmit the parasite at low levels of long-term infection, parasitemia levels during the chronic phase of infection have never been quantified, nor has transmission been demonstrated. To address these epidemiologically significant questions, we established long-term B. equi infections (>1 year), measured parasitemia levels over time, and tested whether nymphal Boophilus microplus ticks could acquire and, after molting to the adult stage, transmit B. equi to naive horses. B. equi levels during the chronic phase of infection ranged from 10(3.3) to 10(6.0)/ml of blood, with fluctuation over time within individual horses. B. microplus ticks fed on chronically infected horses with mean parasite levels of 10(5.5) +/- 10(0.48)/ml of blood acquired B. equi, with detection of B. equi in the salivary glands of 7 to 50% of fed ticks, a range encompassing the percentage of positive ticks that had been identically fed on a horse in the acute phase of infection with high parasitemia levels. Ticks that acquired B. equi from chronically infected horses, as well as those fed during the acute phase of infection, successfully transmitted the parasite to naive horses. The results unequivocally demonstrated that chronically infected horses with low-level parasitemia are competent mammalian reservoirs for tick transmission of B. equi.
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Publication Date: 2005-08-06 PubMed ID: 16081906PubMed Central: PMC1233951DOI: 10.1128/JCM.43.8.3755-3759.2005Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • U.S. Gov't
  • Non-P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigated the ability of tick Boophilus microplus to pick up and transmit Babesia equi, a protozoan parasite, after feeding on horses which were suffering from low-level, long-term infection of the same parasite. This study concluded that these ticks can effectively transfer the parasite from chronically infected horses to unaffected ones.

Study Design

  • The research was designed to fill the knowledge gap regarding the parasitemia levels (the level of parasites in blood) during the chronic phase of B. equi infection in horses.
  • The researchers established long-term (>1 year) B. equi infections in horses, measured parasitemia levels over time, and examined if nymph-stage B. microplus ticks feeding on those horses could acquire and spread the disease to other horses.

Measurements and Findings

  • The study showed that B. equi levels during the chronic phase fluctuated within horses, with an identified range from 10(3.3) to 10(6.0)/ml of blood.
  • When B. microplus ticks fed on these chronic horses, they managed to acquire B. equi. The parasite was detected in the salivary glands of 7-50% of ticks, which spanned the range of earlier observed infection rates in ticks fed on horses suffering from high parasitemia during the acute phase.

Significance of Findings

  • This paper clearly demonstrated that horses with a long-term, low-level infection can act as competent hosts for ticks to acquire and subsequently transmit the B. equi parasite.
  • The findings are substantial, as restrictions put on the movement of chronically infected horses are based on the belief that ticks can pick up and transmit the parasite even at low-long term infection levels.

Conclusion

  • The study’s results confirm that the tick species Boophilus microplus can effectively transmit the protozoan parasite Babesia equi from chronically infected horses to uninfected ones, regardless of the parasitemia levels in the host horses.

Cite This Article

APA
Ueti MW, Palmer GH, Kappmeyer LS, Statdfield M, Scoles GA, Knowles DP. (2005). Ability of the vector tick Boophilus microplus to acquire and transmit Babesia equi following feeding on chronically infected horses with low-level parasitemia. J Clin Microbiol, 43(8), 3755-3759. https://doi.org/10.1128/JCM.43.8.3755-3759.2005

Publication

Journal of clinical microbiology
ISSN: 0095-1137
NlmUniqueID: 7505564
Country: United States
Language: English
Volume: 43
Issue: 8
Pages: 3755-3759

Researcher Affiliations

Ueti, Massaro W
  • Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040, USA. massaro@vetmed.wsu.edu
Palmer, Guy H
    Kappmeyer, Lowell S
      Statdfield, Mary
        Scoles, Glen A
          Knowles, Donald P

            MeSH Terms

            • Animals
            • Arthropod Vectors / parasitology
            • Babesiosis / transmission
            • Babesiosis / veterinary
            • Chronic Disease
            • Horse Diseases / transmission
            • Horses
            • Parasitemia / transmission
            • Polymerase Chain Reaction
            • Ticks / parasitology

            References

            This article includes 27 references
            1. Allred DR, Al-Khedery B. Antigenic variation and cytoadhesion in Babesia bovis and Plasmodium falciparum: different logics achieve the same goal.. Mol Biochem Parasitol 2004 Mar;134(1):27-35.
              pubmed: 14747140doi: 10.1016/j.molbiopara.2003.09.012google scholar: lookup
            2. Allred DR, Cinque RM, Lane TJ, Ahrens KP. Antigenic variation of parasite-derived antigens on the surface of Babesia bovis-infected erythrocytes.. Infect Immun 1994 Jan;62(1):91-8.
              pmc: PMC186072pubmed: 8262654doi: 10.1128/iai.62.1.91-98.1994google scholar: lookup
            3. Barbour AG, Restrepo BI. Antigenic variation in vector-borne pathogens.. Emerg Infect Dis 2000 Sep-Oct;6(5):449-57.
              pmc: PMC2627965pubmed: 10998374doi: 10.3201/eid0605.000502google scholar: lookup
            4. Crampton AL, Miller C, Baxter GD, Barker SC. Expressed sequenced tags and new genes from the cattle tick, Boophilus microplus.. Exp Appl Acarol 1998 Mar;22(3):177-86.
              pubmed: 9519466doi: 10.1023/a:1006058113409google scholar: lookup
            5. Eriks IS, Stiller D, Palmer GH. Impact of persistent Anaplasma marginale rickettsemia on tick infection and transmission.. J Clin Microbiol 1993 Aug;31(8):2091-6.
              pmc: PMC265702pubmed: 8370734doi: 10.1128/jcm.31.8.2091-2096.1993google scholar: lookup
            6. Frerichs WM, Holbrook AA, Johnson AJ. Equine piroplasmosis: production of antigens for the complement-fixation test.. Am J Vet Res 1969 Aug;30(8):1337-41.
              pubmed: 5816365
            7. Friedhoff KT. Transmission of Babesia. 1988 p. 23-52.
            8. Friedhoff KT, Tenter AM, Müller I. Haemoparasites of equines: impact on international trade of horses.. Rev Sci Tech 1990 Dec;9(4):1187-94.
              pubmed: 2132711
            9. Guimarães AM, Lima JD, Ribeiro MF. Sporogony and experimental transmission of Babesia equi by Boophilus microplus.. Parasitol Res 1998;84(4):323-7.
              pubmed: 9580425doi: 10.1007/s004360050404google scholar: lookup
            10. Horrocks P, Pinches R, Christodoulou Z, Kyes SA, Newbold CI. Variable var transition rates underlie antigenic variation in malaria.. Proc Natl Acad Sci U S A 2004 Jul 27;101(30):11129-34.
              pmc: PMC503751pubmed: 15256597doi: 10.1073/pnas.0402347101google scholar: lookup
            11. Kappmeyer LS, Perryman LE, Knowles DP Jr. A Babesia equi gene encodes a surface protein with homology to Theileria species.. Mol Biochem Parasitol 1993 Nov;62(1):121-4.
              pubmed: 8114813doi: 10.1016/0166-6851(93)90185-zgoogle scholar: lookup
            12. Karakashian SJ, Rudzinska MA, Spielman A, Lewengrub S, Piesman J, Shoukrey N. Ultrastructural studies on sporogony of Babesia microti in salivary gland cells of the tick Ixodes dammini.. Cell Tissue Res 1983;231(2):275-87.
              pubmed: 6850804doi: 10.1007/bf00222180google scholar: lookup
            13. Knowles DP, Kappmeyer LS, Perryman LE. Genetic and biochemical analysis of erythrocyte-stage surface antigens belonging to a family of highly conserved proteins of Babesia equi and Theileria species.. Mol Biochem Parasitol 1997 Dec 1;90(1):69-79.
              pubmed: 9497033doi: 10.1016/s0166-6851(97)00150-3google scholar: lookup
            14. Knowles DP Jr, Kappmeyer LS, Stiller D, Hennager SG, Perryman LE. Antibody to a recombinant merozoite protein epitope identifies horses infected with Babesia equi.. J Clin Microbiol 1992 Dec;30(12):3122-6.
              pmc: PMC270599pubmed: 1280648doi: 10.1128/jcm.30.12.3122-3126.1992google scholar: lookup
            15. Knowles DP Jr, Perryman LE, Goff WL, Miller CD, Harrington RD, Gorham JR. A monoclonal antibody defines a geographically conserved surface protein epitope of Babesia equi merozoites.. Infect Immun 1991 Jul;59(7):2412-7.
              pmc: PMC258026pubmed: 1711016doi: 10.1128/iai.59.7.2412-2417.1991google scholar: lookup
            16. Knowles DP Jr, Perryman LE, Kappmeyer LS, Hennager SG. Detection of equine antibody to Babesia equi merozoite proteins by a monoclonal antibody-based competitive inhibition enzyme-linked immunosorbent assay.. J Clin Microbiol 1991 Sep;29(9):2056-8.
              pmc: PMC270260pubmed: 1774334doi: 10.1128/jcm.29.9.2056-2058.1991google scholar: lookup
            17. Mehlhorn H, Schein E. Redescription of Babesia equi Laveran, 1901 as Theileria equi Mehlhorn, Schein 1998.. Parasitol Res 1998 Jun;84(6):467-75.
              pubmed: 9660136doi: 10.1007/s004360050431google scholar: lookup
            18. O'Connor RM, Lane TJ, Stroup SE, Allred DR. Characterization of a variant erythrocyte surface antigen (VESA1) expressed by Babesia bovis during antigenic variation.. Mol Biochem Parasitol 1997 Nov;89(2):259-70.
              pubmed: 9364970doi: 10.1016/s0166-6851(97)00125-4google scholar: lookup
            19. Riek RF. The cycle of Babesia bigemina (Smith and Kilborne, 1893) in the tick vector Boophilus microplus (Canestrini). Aust. J. Agric. Res. 1964 15:802-821.
            20. Rudzinska MA, Lewengrub S, Spielman A, Piesman J. Invasion of Babesia microti into epithelial cells of the tick gut.. J Protozool 1983 May;30(2):338-46.
              pubmed: 6631777doi: 10.1111/j.1550-7408.1983.tb02927.xgoogle scholar: lookup
            21. Rudzinska MA, Spielman A, Lewengrub S, Trager W, Piesman J. Sexuality in piroplasms as revealed by electron microscopy in Babesia microti.. Proc Natl Acad Sci U S A 1983 May;80(10):2966-70.
              pmc: PMC393954pubmed: 6574467doi: 10.1073/pnas.80.10.2966google scholar: lookup
            22. Schein E. Equine babesiosis. 1988 p. 197-208.
            23. Shkap V, Cohen I, Leibovitz B, Savitsky, Pipano E, Avni G, Shofer S, Giger U, Kappmeyer L, Knowles D. Seroprevalence of Babesia equi among horses in Israel using competitive inhibition ELISA and IFA assays.. Vet Parasitol 1998 Apr 30;76(4):251-9.
              pubmed: 9650862doi: 10.1016/s0304-4017(97)00228-8google scholar: lookup
            24. Stiller D, Goff WL, Johnson LW, Knowles DP. Dermacentor variabilis and boophilus microplus (Acari: Ixodidae): experimental vectors of Babesia equi to equids.. J Med Entomol 2002 Jul;39(4):667-70.
              pubmed: 12144301doi: 10.1603/0022-2585-39.4.667google scholar: lookup
            25. Telford SR 3rd, Spielman A. Reservoir competence of white-footed mice for Babesia microti.. J Med Entomol 1993 Jan;30(1):223-7.
              pubmed: 8433329doi: 10.1093/jmedent/30.1.223google scholar: lookup
            26. Ueti MW, Palmer GH, Kappmeyer LS, Scoles GA, Knowles DP. Expression of equi merozoite antigen 2 during development of Babesia equi in the midgut and salivary gland of the vector tick Boophilus microplus.. J Clin Microbiol 2003 Dec;41(12):5803-9.
              pmc: PMC308990pubmed: 14662988doi: 10.1128/jcm.41.12.5803-5809.2003google scholar: lookup
            27. Zapf F, Schein E. The development of Babesia (Theileria) equi (Laveran, 1901) in the gut and the haemolymph of the vector ticks, Hyalomma species.. Parasitol Res 1994;80(4):297-302.
              pubmed: 8073015doi: 10.1007/bf02351869google scholar: lookup

            Citations

            This article has been cited 33 times.
            1. Díaz-Sánchez AA, Obregón D, Santos HA, Corona-González B. Advances in the Epidemiological Surveillance of Tick-Borne Pathogens. Pathogens 2023 Apr 23;12(5).
              doi: 10.3390/pathogens12050633pubmed: 37242303google scholar: lookup
            2. Onzere CK, Herndon DR, Hassan A, Oyen K, Poh KC, Scoles GA, Fry LM. A U.S. Isolate of Theileria orientalis Ikeda Is Not Transstadially Transmitted to Cattle by Rhipicephalus microplus. Pathogens 2023 Apr 5;12(4).
              doi: 10.3390/pathogens12040559pubmed: 37111445google scholar: lookup
            3. Peckle M, Santos H, Pires M, Silva C, Costa R, Vitari G, Camilo T, Meireles N, Paulino P, Massard C. Dynamics of Theileria equi Infection in Rhipicephalus (Boophilus) microplus during the Parasitic Phase in a Chronically Infected Horse. Pathogens 2022 Apr 29;11(5).
              doi: 10.3390/pathogens11050525pubmed: 35631046google scholar: lookup
            4. Coultous RM, Sutton DGM, Boden LA. A risk assessment of equine piroplasmosis entry, exposure and consequences in the UK. Equine Vet J 2023 Mar;55(2):282-294.
              doi: 10.1111/evj.13579pubmed: 35478189google scholar: lookup
            5. Salinas-Estrella E, Ueti MW, Lobanov VA, Castillo-Payró E, Lizcano-Mata A, Badilla C, Martínez-Ibáñez F, Mosqueda J. Serological and molecular detection of Babesia caballi and Theileria equi in Mexico: A prospective study. PLoS One 2022;17(3):e0264998.
              doi: 10.1371/journal.pone.0264998pubmed: 35259206google scholar: lookup
            6. Onyiche TE, Igwenagu E, Malgwi SA, Omeh IJ, Biu AA, Thekisoe O. Hematology and biochemical values in equines naturally infected with Theileria equi in Nigeria. Trop Anim Health Prod 2022 Feb 14;54(2):103.
              doi: 10.1007/s11250-022-03095-wpubmed: 35157179google scholar: lookup
            7. Schnittger L, Ganzinelli S, Bhoora R, Omondi D, Nijhof AM, Florin-Christensen M. The Piroplasmida Babesia, Cytauxzoon, and Theileria in farm and companion animals: species compilation, molecular phylogeny, and evolutionary insights. Parasitol Res 2022 May;121(5):1207-1245.
              doi: 10.1007/s00436-022-07424-8pubmed: 35098377google scholar: lookup
            8. Onzere CK, Fry LM, Bishop RP, Silva MG, Bastos RG, Knowles DP, Suarez CE. Theileria equi claudin like apicomplexan microneme protein contains neutralization-sensitive epitopes and interacts with components of the equine erythrocyte membrane skeleton. Sci Rep 2021 Apr 29;11(1):9301.
              doi: 10.1038/s41598-021-88902-4pubmed: 33927329google scholar: lookup
            9. Idoko IS, Edeh RE, Adamu AM, Machunga-Mambula S, Okubanjo OO, Balogun EO, Adamu S, Johnson W, Kappmeyer L, Mousel M, Ueti MW. Molecular and Serological Detection of Piroplasms in Horses from Nigeria. Pathogens 2021 Apr 23;10(5).
              doi: 10.3390/pathogens10050508pubmed: 33922468google scholar: lookup
            10. Dinkel KD, Herndon DR, Noh SM, Lahmers KK, Todd SM, Ueti MW, Scoles GA, Mason KL, Fry LM. A U.S. isolate of Theileria orientalis, Ikeda genotype, is transmitted to cattle by the invasive Asian longhorned tick, Haemaphysalis longicornis. Parasit Vectors 2021 Mar 16;14(1):157.
              doi: 10.1186/s13071-021-04659-9pubmed: 33726815google scholar: lookup
            11. Paulino P, Vitari G, Rezende A, Couto J, Antunes S, Domingos A, Peckle M, Massard C, Araújo F, Santos H. Characterization of the Rhipicephalus (Boophilus) microplus Sialotranscriptome Profile in Response to Theileria equi Infection. Pathogens 2021 Feb 4;10(2).
              doi: 10.3390/pathogens10020167pubmed: 33557100google scholar: lookup
            12. Mshelia PW, Kappmeyer L, Johnson WC, Kudi CA, Oluyinka OO, Balogun EO, Richard EE, Onoja E, Sears KP, Ueti MW. Molecular detection of Theileria species and Babesia caballi from horses in Nigeria. Parasitol Res 2020 Sep;119(9):2955-2963.
              doi: 10.1007/s00436-020-06797-ypubmed: 32647992google scholar: lookup
            13. Tirosh-Levy S, Steinman A, Levy H, Katz Y, Shtilman M, Gottlieb Y. Parasite load and genotype are associated with clinical outcome of piroplasm-infected equines in Israel. Parasit Vectors 2020 May 20;13(1):267.
              doi: 10.1186/s13071-020-04133-ypubmed: 32434550google scholar: lookup
            14. Tirosh-Levy S, Gottlieb Y, Mimoun L, Mazuz ML, Steinman A. Transplacental Transmission of Theileria equi Is Not a Common Cause of Abortions and Infection of Foals in Israel. Animals (Basel) 2020 Feb 21;10(2).
              doi: 10.3390/ani10020341pubmed: 32098113google scholar: lookup
            15. Smith RM, Bhoora RV, Kotzé A, Grobler JP, Lee Dalton D. Translocation a potential corridor for equine piroplasms in Cape mountain zebra (Equus zebra zebra). Int J Parasitol Parasites Wildl 2019 Aug;9:130-133.
              doi: 10.1016/j.ijppaw.2019.04.010pubmed: 31080728google scholar: lookup
            16. Lobanov VA, Peckle M, Massard CL, Brad Scandrett W, Gajadhar AA. Development and validation of a duplex real-time PCR assay for the diagnosis of equine piroplasmosis. Parasit Vectors 2018 Mar 2;11(1):125.
              doi: 10.1186/s13071-018-2751-6pubmed: 29499748google scholar: lookup
            17. Gimenez F, Hines SA, Evanoff R, Ojo KK, Van Voorhis WC, Maly DJ, Vidadala RSR, Mealey RH. In vitro growth inhibition of Theileria equi by bumped kinase inhibitors. Vet Parasitol 2018 Feb 15;251:90-94.
              doi: 10.1016/j.vetpar.2017.12.024pubmed: 29426483google scholar: lookup
            18. Hines SA, Ramsay JD, Kappmeyer LS, Lau AO, Ojo KK, Van Voorhis WC, Knowles DP, Mealey RH. Theileria equi isolates vary in susceptibility to imidocarb dipropionate but demonstrate uniform in vitro susceptibility to a bumped kinase inhibitor. Parasit Vectors 2015 Jan 20;8:33.
              doi: 10.1186/s13071-014-0611-6pubmed: 25600252google scholar: lookup
            19. Scoles GA, Ueti MW. Amblyomma cajennense is an intrastadial biological vector of Theileria equi. Parasit Vectors 2013 Oct 23;6(1):306.
              doi: 10.1186/1756-3305-6-306pubmed: 24499587google scholar: lookup
            20. Ramsay JD, Ueti MW, Johnson WC, Scoles GA, Knowles DP, Mealey RH. Lymphocytes and macrophages are infected by Theileria equi, but T cells and B cells are not required to establish infection in vivo. PLoS One 2013;8(10):e76996.
              doi: 10.1371/journal.pone.0076996pubmed: 24116194google scholar: lookup
            21. Awinda PO, Mealey RH, Williams LB, Conrad PA, Packham AE, Reif KE, Grause JF, Pelzel-McCluskey AM, Chung C, Bastos RG, Kappmeyer LS, Howe DK, Ness SL, Knowles DP, Ueti MW. Serum antibodies from a subset of horses positive for Babesia caballi by competitive enzyme-linked immunosorbent assay demonstrate a protein recognition pattern that is not consistent with infection. Clin Vaccine Immunol 2013 Nov;20(11):1752-7.
              doi: 10.1128/CVI.00479-13pubmed: 24049108google scholar: lookup
            22. Peckle M, Pires MS, Dos Santos TM, Roier EC, da Silva CB, Vilela JA, Santos HA, Massard CL. Molecular epidemiology of Theileria equi in horses and their association with possible tick vectors in the state of Rio de Janeiro, Brazil. Parasitol Res 2013 May;112(5):2017-25.
              doi: 10.1007/s00436-013-3360-0pubmed: 23474658google scholar: lookup
            23. Hall CM, Busch JD, Scoles GA, Palma-Cagle KA, Ueti MW, Kappmeyer LS, Wagner DM. Genetic characterization of Theileria equi infecting horses in North America: evidence for a limited source of U.S. introductions. Parasit Vectors 2013 Feb 11;6:35.
              doi: 10.1186/1756-3305-6-35pubmed: 23399005google scholar: lookup
            24. Kappmeyer LS, Thiagarajan M, Herndon DR, Ramsay JD, Caler E, Djikeng A, Gillespie JJ, Lau AO, Roalson EH, Silva JC, Silva MG, Suarez CE, Ueti MW, Nene VM, Mealey RH, Knowles DP, Brayton KA. Comparative genomic analysis and phylogenetic position of Theileria equi. BMC Genomics 2012 Nov 9;13:603.
              doi: 10.1186/1471-2164-13-603pubmed: 23137308google scholar: lookup
            25. Ueti MW, Mealey RH, Kappmeyer LS, White SN, Kumpula-McWhirter N, Pelzel AM, Grause JF, Bunn TO, Schwartz A, Traub-Dargatz JL, Hendrickson A, Espy B, Guthrie AJ, Fowler WK, Knowles DP. Re-emergence of the apicomplexan Theileria equi in the United States: elimination of persistent infection and transmission risk. PLoS One 2012;7(9):e44713.
              doi: 10.1371/journal.pone.0044713pubmed: 22970295google scholar: lookup
            26. Ueti MW, Palmer GH, Scoles GA, Kappmeyer LS, Knowles DP. Persistently infected horses are reservoirs for intrastadial tick-borne transmission of the apicomplexan parasite Babesia equi. Infect Immun 2008 Aug;76(8):3525-9.
              doi: 10.1128/IAI.00251-08pubmed: 18490466google scholar: lookup
            27. Ueti MW, Reagan JO Jr, Knowles DP Jr, Scoles GA, Shkap V, Palmer GH. Identification of midgut and salivary glands as specific and distinct barriers to efficient tick-borne transmission of Anaplasma marginale. Infect Immun 2007 Jun;75(6):2959-64.
              doi: 10.1128/IAI.00284-07pubmed: 17420231google scholar: lookup
            28. Cunha CW, McGuire TC, Kappmeyer LS, Hines SA, Lopez AM, Dellagostin OA, Knowles DP. Development of specific immunoglobulin Ga (IgGa) and IgGb antibodies correlates with control of parasitemia in Babesia equi Infection. Clin Vaccine Immunol 2006 Feb;13(2):297-300.
              doi: 10.1128/CVI.13.2.297-300.2006pubmed: 16467341google scholar: lookup
            29. Poh KC, Oyen K, Onzere CK, Kappmeyer LS, Bastos RG. Haemaphysalis longicornis ticks are unable to transstadially transmit Theileria haneyi to horses. Front Vet Sci 2025;12:1572944.
              doi: 10.3389/fvets.2025.1572944pubmed: 40241805google scholar: lookup
            30. Fernandes TA, Paulino PG, Dos Santos Juliano D, Rabello CA, de Oliveira NVB, de Souza Santana M, Peckle M, Massard CL, da Costa Angelo I, Jacob JCF, Santos HA. Epidemiology and genetic diversity of Theileria equi and Babesia caballi in draft horses in the Distrito Federal, Brazil. Trop Anim Health Prod 2025 Feb 19;57(2):72.
              doi: 10.1007/s11250-025-04321-xpubmed: 39969660google scholar: lookup
            31. Onzere CK, Bastos RG, Bishop RP, Suarez CE, Fry LM. Expression of IL-10 and TGF-β1 in horses experimentally infected with T. equi merozoites is associated with antibody production but not modulation of pro-inflammatory responses. Front Immunol 2024;15:1370255.
              doi: 10.3389/fimmu.2024.1370255pubmed: 38803499google scholar: lookup
            32. Ahedor B, Otgonsuren D, Zhyldyz A, Guswanto A, Ngigi NMM, Valinotti MFR, Kothalawala H, Kalaichelvan N, Silva SSP, Kothalawala H, Acosta TJ, Sivakumar T, Yokoyama N. Development and evaluation of specific polymerase chain reaction assays for detecting Theileria equi genotypes. Parasit Vectors 2023 Nov 25;16(1):435.
              doi: 10.1186/s13071-023-06045-zpubmed: 38007442google scholar: lookup
            33. Ghafar A, Cabezas-Cruz A. Ticking off the Tick Vectors: Rhipicephalus microplus Fails to Transmit Theileria orientalis. Pathogens 2023 Nov 3;12(11).
              doi: 10.3390/pathogens12111311pubmed: 38003776google scholar: lookup
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