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Home / Equine Research Bank / PLoS One / Accurate determination of phenotypic information from histor...
PloS one2010; 5(12); e15172; doi: 10.1371/journal.pone.0015172

Accurate determination of phenotypic information from historic thoroughbred horses by single base extension.

Abstract: Historic DNA data have the potential to identify phenotypic information otherwise invisible in the historical, archaeological and palaeontological record. In order to determine whether a single nucleotide polymorphism typing protocol based on single based extension (SNaPshot™) could produce reliable phenotypic data from historic samples, we genotyped three coat colour markers for a sample of historic Thoroughbred horses for which both phenotypic and correct genotypic information were known from pedigree information in the General Stud Book. Experimental results were consistent with the pedigrees in all cases. Thus we demonstrate that historic DNA techniques can produce reliable phenotypic information from museum specimens.
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Publication Date: 2010-12-02 PubMed ID: 21152043PubMed Central: PMC2996296DOI: 10.1371/journal.pone.0015172Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research study investigates the possibility of accurately determining phenotypic characteristics of historic thoroughbred horses using a DNA analysis technique called single base extension. The results indicate that this technique can produce reliable data from historic specimens by comparing the obtained genotypic information with recorded pedigree data.

Overview of the Research

  • The authors kicked off their research by stating the significance of historical DNA data, emphasizing its potential to provide insights into phenotypic details that can’t be deciphered from historical, archaeological, and paleontological records.
  • They set out to test if a Single Nucleotide Polymorphism (SNP) typing protocol based on single base extension (specifically, a method named SNaPshot™) could provide reliable phenotypic data from historic samples.

Methodology and Results

  • For the experimental process, they chose to work on a sample of historic Thoroughbred horses whose phenotypic and correct genotypic information were pre-known from pedigree records documented in the General Stud Book.
  • They focused mostly on genotyping three markers associated with coat color because this is one of the most prominent observable characteristics in horses.
  • Successfully, the experimental results they acquired were consistent with the information in the pedigrees for all the tested samples. Consequently, they concluded that using DNA techniques on historic samples could indeed provide trustworthy and accurate phenotypic information.

Implication and Contribution of the Study

  • This study’s findings demonstrate that genotyping protocols like SNaPshot™ can effectively unveil hidden phenotypic details from historic biological specimens, thereby adding a potent tool to the arsenal of geneticists working with historic or ancient DNA samples.
  • Besides its academic implications, this research’s results can be used in diverse fields, including archaeology, paleontology, animal breeding, and potentially, in understanding human ancestry and diseases.

Cite This Article

APA
Campana MG, Whitten CM, Edwards CJ, Stock F, Murphy AM, Binns MM, Barker GW, Bower MA. (2010). Accurate determination of phenotypic information from historic thoroughbred horses by single base extension. PLoS One, 5(12), e15172. https://doi.org/10.1371/journal.pone.0015172

Publication

PloS one
ISSN: 1932-6203
NlmUniqueID: 101285081
Country: United States
Language: English
Volume: 5
Issue: 12
Pages: e15172
PII: e15172

Researcher Affiliations

Campana, Michael G
  • Department of Archaeology, University of Cambridge, Cambridge, United Kingdom. mgc32@cam.ac.uk
Whitten, C Mark
    Edwards, Ceiridwen J
      Stock, Frauke
        Murphy, Angela M
          Binns, Matthew M
            Barker, Graeme W W
              Bower, Mim A

                MeSH Terms

                • Alleles
                • Animals
                • DNA / genetics
                • DNA Primers / genetics
                • Genotype
                • Horses / genetics
                • Paleontology / methods
                • Pedigree
                • Phenotype
                • Polymerase Chain Reaction / methods
                • Polymorphism, Single Nucleotide
                • Sequence Analysis, DNA / methods

                Conflict of Interest Statement

                The authors have declared that no competing interests exist.

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                Citations

                This article has been cited 3 times.
                1. Paul S, Gangwar A, Arya A, Bhargava K, Ahmad Y. Modulation of lung cytoskeletal remodeling, RXR based metabolic cascades and inflammation to achieve redox homeostasis during extended exposures to lowered pO(2).. Apoptosis 2021 Aug;26(7-8):431-446.
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                3. Bower MA, McGivney BA, Campana MG, Gu J, Andersson LS, Barrett E, Davis CR, Mikko S, Stock F, Voronkova V, Bradley DG, Fahey AG, Lindgren G, MacHugh DE, Sulimova G, Hill EW. The genetic origin and history of speed in the Thoroughbred racehorse.. Nat Commun 2012 Jan 24;3:643.
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