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The Journal of antimicrobial chemotherapy2011; 66(10); 2248-2254; doi: 10.1093/jac/dkr289

Acinetobacter baumannii isolates from pets and horses in Switzerland: molecular characterization and clinical data.

Abstract: We investigated whether Acinetobacter baumannii isolates of veterinary origin shared common molecular characteristics with those described in humans. Methods: Nineteen A. baumannii isolates collected in pets and horses were analysed. Clonality was studied using repetitive extragenic palindromic PCR (rep-PCR) and multilocus sequence typing (MLST). PCR and DNA sequencing for various β-lactamase, aminoglycoside-modifying enzyme, gyrA and parC, ISAba1 and IS1133, adeR and adeS of the AdeABC efflux pump, carO porin and class 1/2/3 integron genes were performed. Results: Two main clones [A (n = 8) and B (n = 9)] were observed by rep-PCR. MLST indicated that clone A contained isolates of sequence type (ST) ST12 (international clone II) and clone B contained isolates of ST15 (international clone I). Two isolates of ST10 and ST20 were also noted. Seventeen isolates were resistant to gentamicin, 12 to ciprofloxacin and 3 to carbapenems. Isolates of ST12 carried bla(OXA-66), bla(ADC-25), bla(TEM-1), aacC2 and IS1133. Strains of ST15 possessed bla(OXA-69), bla(ADC-11), bla(TEM-1) and a class 1 integron carrying aacC1 and aadA1. ISAba1 was found upstream of bla(ADC) (one ST10 and one ST12) and/or bla(OXA-66) (seven ST12). Twelve isolates of different STs contained the substitutions Ser83Leu in GyrA and Ser80Leu or Glu84Lys in ParC. Significant disruptions of CarO porin and overexpressed efflux pumps were not observed. The majority of infections were hospital acquired and in animals with predisposing conditions for infection. Conclusions: STs and the molecular background of resistance observed in our collection have been frequently described in A. baumannii detected in human patients. Animals should be considered as a potential reservoir of multidrug-resistant A. baumannii.
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Publication Date: 2011-07-06 PubMed ID: 21733964PubMed Central: PMC3172040DOI: 10.1093/jac/dkr289Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research study focuses on investigating if Acinetobacter baumannii, a bacteria found in pets and horses, shares molecular characteristics with strains found in humans, with the hopes of understanding more about antibiotic resistance and bacterial transmission.

Methods

The researchers used several methods ranging from collection and analysis of A. baumannii isolates to various techniques of DNA sequencing.

  • Nineteen isolates of A. baumannii were collected from pets and horses for examination.
  • Repetitive extragenic palindromic PCR (rep-PCR) and multilocus sequence typing (MLST) methods were utilized for studying clonality of the samples.
  • Various gene sequences were examined using PCR and DNA sequencing, these genes play a role in antibiotic resistance.

Results

The outcomes of the research presented insights about the genetic characteristics and antibiotic resistance patterns of A. baumannii isolates.

  • Two main clones A and B were identified using rep-PCR, clone A containing isolates of sequence type ST12 and clone B containing those of ST15. Some isolates of ST10 and ST20 were also observed.
  • Most isolates displayed resistance to various antibiotics: 17 isolates to gentamicin, 12 to ciprofloxacin, and 3 to carbapenems.
  • Different isolates carried various antibiotic-resistance genes such as blaOXA-66, blaADC-25, blaTEM-1, associated with ST12 and blaOXA-69, blaADC-11, blaTEM-1 related to ST15.
  • Significant disruptions of CarO porin (part of bacterial cell wall) and overexpressed efflux pumps (part of the antibiotic-resistance mechanism) were not seen.
  • Interestingly, a majority of infections were hospital-acquired and seen in animals having predisposing conditions for infection.

Conclusions

The sequence types and molecular features linked to resistance observed in A. baumannii obtained from animals were similar to those found in human patients.

  • This suggests that pets and horses could potentially serve as a reservoir for the multidrug-resistant A. baumannii, indicating a potential risk of antibiotic-resistant bacterial transmission between animals and humans.
  • This enforces the importance of implementing strict antibiotic policies not only in human healthcare settings but also in veterinary medicine to curb the rise and spread of antibiotic resistance.

Cite This Article

APA
Endimiani A, Hujer KM, Hujer AM, Bertschy I, Rossano A, Koch C, Gerber V, Francey T, Bonomo RA, Perreten V. (2011). Acinetobacter baumannii isolates from pets and horses in Switzerland: molecular characterization and clinical data. J Antimicrob Chemother, 66(10), 2248-2254. https://doi.org/10.1093/jac/dkr289

Publication

The Journal of antimicrobial chemotherapy
ISSN: 1460-2091
NlmUniqueID: 7513617
Country: England
Language: English
Volume: 66
Issue: 10
Pages: 2248-2254

Researcher Affiliations

Endimiani, Andrea
  • Institute of Veterinary Bacteriology, University of Bern, Länggass-Strasse 122, CH-3001 Bern, Switzerland. aendimiani@gmail.com
Hujer, Kristine M
    Hujer, Andrea M
      Bertschy, Isabelle
        Rossano, Alexandra
          Koch, Christoph
            Gerber, Vinzenz
              Francey, Thierry
                Bonomo, Robert A
                  Perreten, Vincent

                    MeSH Terms

                    • Acinetobacter Infections / drug therapy
                    • Acinetobacter Infections / microbiology
                    • Acinetobacter Infections / veterinary
                    • Acinetobacter baumannii / genetics
                    • Acinetobacter baumannii / isolation & purification
                    • Acinetobacter baumannii / pathogenicity
                    • Animals
                    • Anti-Bacterial Agents / pharmacology
                    • Bacterial Typing Techniques
                    • Base Sequence
                    • DNA, Bacterial / genetics
                    • Disease Reservoirs / microbiology
                    • Disease Reservoirs / veterinary
                    • Drug Resistance, Multiple, Bacterial / genetics
                    • Horses / microbiology
                    • Microbial Sensitivity Tests
                    • Multilocus Sequence Typing
                    • Pets / microbiology
                    • Phenotype
                    • Sequence Analysis, DNA
                    • Switzerland

                    Grant Funding

                    • AI063517-07 / NIAID NIH HHS
                    • AI072219-05 / NIAID NIH HHS

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