Acute phase proteins in racehorses with inflammatory airway disease.
Abstract: Systemic inflammation is observed in horses with heaves and could also be present in horses with a lesser degree of pulmonary inflammation. Objective: It was hypothesized that racehorses with inflammatory airway disease (IAD) have increased concentration of circulating acute phase proteins. The objective of this study was to compare serum acute phase proteins of racehorses with and without lower airway inflammation. Methods: Serum from 21 client-owned Standardbred racehorses with exercise intolerance and lower airway inflammation and serum from 10 client-owned Standardbred racehorses with exercise intolerance without lower airway inflammation. Methods: In a case-control study, serum samples from previously characterized horses presented for exercise intolerance with or without lower airway inflammation based on bronchoalveolar lavage fluid cytology were analyzed for serum amyloid A protein (SAA), C-reactive protein (CRP), and haptoglobin using commercial ELISAs. Results: There was no significant differences between groups for SAA (non-IAD versus IAD, median (range): 3.47 (0.06-34.94) versus 6.33 (0.06-80) μg/mL, P = .49), CRP (10.87 (2.05-29.03) versus 4.63 (0.02-31.81) μg/mL, P = .23) or haptoglobin (900.36 (607.99-2018.84) versus 749.54 (530.81-1076.95) μg/mL, P = .09). Conclusions: In this population of poorly performing racehorses in training, serum SAA, CRP, and haptoglobin were not helpful in distinguishing between horses with IAD from horses with exercise intolerance from other causes.
Copyright © 2015 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of the American College of Veterinary Internal Medicine.
Publication Date: 2015-04-09 PubMed ID: 25857218PubMed Central: PMC4895423DOI: 10.1111/jvim.12587Google Scholar: Lookup
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- Journal Article
- Acute Phase Proteins
- Airway Disease
- Bronchoalveolar Lavage
- Case-Control Study
- Clinical Study
- Diagnostic Technique
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Equine Health
- Exercise Intolerance
- Exercise Physiology
- Haptoglobin
- Inflammation
- Pulmonary Health
- Racehorses
- Respiratory Disease
- Serum
- Serum Amyloid A
- Standardbred Horses
Summary
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The research explores the levels of acute phase proteins in racehorses suffering from inflammatory airway disease (IAD). It concludes that these protein levels were not significantly different in horses with and without the disease, so these markers may not be used to distinguish between horses with IAD and those experiencing exercise intolerance due to other reasons.
Objective and Hypothesis
- The researchers hypothesized that racehorses with IAD have higher concentration of circulating acute phase proteins.
- The study aimed to establish if specific acute phase proteins could be used as reliable indicators of IAD.
Methods
- The study compared the serum acute phase proteins (SAA, CRP, and haptoglobin) of two groups of horses: one with lower airway inflammation (21 horses) and the other (10 horses) without it.
- All tested horses were Standardbred racehorses with exercise intolerance.
- A case-control study was performed and serum samples from horses with distinct performance characteristics were analyzed using commercial ELISA kits.
- The presence of airway inflammation in the horses was identified through bronchoalveolar lavage fluid cytology.
Findings
- No significant difference was found in the concentrations of SAA, CRP, and haptoglobin between two groups of horses.
- The median values for SAA, CRP, and haptoglobin were found to be comparable for both groups, and the differences were not statistically significant (p > 0.05).
Conclusion
- The serum levels of SAA, CRP, and haptoglobin, which are considered as indicators of systemic inflammation, did not prove useful for distinguishing between horses with IAD and those experiencing performance issues due to other causes.
- The findings suggest that other novel indicators need to be explored for accurate diagnosis of IAD in horses.
Cite This Article
APA
Leclere M, Lavoie-Lamoureux A, Lavoie JP.
(2015).
Acute phase proteins in racehorses with inflammatory airway disease.
J Vet Intern Med, 29(3), 940-945.
https://doi.org/10.1111/jvim.12587 Publication
Researcher Affiliations
- Faculty of Veterinary Medicine, Clinical Sciences Department, Université de Montréal, Saint-Hyacinthe, QC, Canada.
- Faculty of Veterinary Medicine, Clinical Sciences Department, Université de Montréal, Saint-Hyacinthe, QC, Canada.
- Faculty of Veterinary Medicine, Clinical Sciences Department, Université de Montréal, Saint-Hyacinthe, QC, Canada.
MeSH Terms
- Acute-Phase Proteins / analysis
- Animals
- Bronchoalveolar Lavage Fluid / cytology
- C-Reactive Protein / analysis
- Case-Control Studies
- Enzyme-Linked Immunosorbent Assay / veterinary
- Female
- Haptoglobins / analysis
- Horse Diseases / blood
- Horses
- Inflammation / blood
- Inflammation / veterinary
- Male
- Respiratory Tract Diseases / blood
- Respiratory Tract Diseases / veterinary
- Serum Amyloid A Protein / analysis
References
This article includes 23 references
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