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Home / Equine Research Bank / Front Vet Sci / Acute phase proteins levels in horses, after a single carboh...
Frontiers in veterinary science2023; 10; 1043656; doi: 10.3389/fvets.2023.1043656

Acute phase proteins levels in horses, after a single carbohydrate overload, associated with cecal alkalinization.

Abstract: Horses submitted to carbohydrate overload can develop laminitis due to changes in cecal pH and microbiota, followed by an increase in transmural absorption of luminal content, including bacterial toxins. In response to acute injury there is hepatic overproduction of several proteins known as acute phase proteins (APP). Few studies have evaluated protein fractionation to characterize the inflammatory response in acute laminitis. The aim of this study was to test the viability of an experimental model to induce acute laminitis, using a single carbohydrate overload, and the influence of a buffering solution on the development of the disease; also, study the kinetics of APP during acute laminitis, as well as the correlation between these proteins and clinical signs associated to this syndrome. Unassigned: Ten healthy horses were divided in a factorial and randomized way into four groups ( = 5): control group (CG), starch group (SG), buffer group (BG), and starch C buffer group (SBG). They were evaluated at seven times (T0h, T4h, T8h, T12h, T24h, T48h, and T72h), which included clinical evaluation and blood sample collection. Total serum protein and albumin concentrations were determined by colorimetry and the other APP by polyacrylamide gel electrophoresis containing sodium dodecyl sulfate and commercial ELISA kits. Data were analyzed by two-way ANOVA, followed by Tukey's test ( 0.05). Transferrin, considered a negative APP, showed a positive response pattern in SG and SBG. Ceruloplasmin had a positive correlation with Obel grade, heart rate on animals from SGB and number of steps on horses submitted to starch overload (SG and SBG). Ceruloplasmin, α-1-antitrypsin and haptoglobin concentrations increased in SBG, suggesting an inflammatory response in animals of this group. Changes in clinical parameters were also more evident in the SBG, corroborating the protein fractionation findings.
Copyright © 2023 Peixoto Rabelo, Barroco de Paula, Carvalho Bustamante, Santana, Gomes da Silva, Baldassi, Canola and Araújo Valadão.
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Publication Date: 2023-02-02 PubMed ID: 36816195PubMed Central: PMC9932335DOI: 10.3389/fvets.2023.1043656Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research examined how high carbohydrate intake in horses might cause acute laminitis, an inflammatory foot condition, due to changes in gut environment and resulting inflammation. It also investigated if the administration of a buffering solution could prevent this condition, and whether acute phase proteins (APP), which are overproduced in response to an injury, correlate with the symptoms of acute laminitis.

Research Methodology

  • The study involved ten healthy horses that were randomly grouped into four categories: control, starch, buffer, and starch and buffer.
  • The horses were observed and evaluated at seven intervals and their blood samples were collected.
  • The concentration of total serum protein and albumin in these samples was measured using a colorimetric method, while the levels of other acute phase proteins were determined by a technique called polyacrylamide gel electrophoresis and commercial ELISA kits.
  • Statistical analysis was then performed on these data to identify any significant differences or correlations.

Findings

  • 40% of the horses in the starch group and 60% in the starch and buffer group developed acute laminitis symptoms.
  • The administration of the buffer solution did not show any effect in preventing the symptoms of laminitis.
  • There was no difference observed in the levels of total serum protein, albumin, serum amyloid A, and C-reactive protein among all the groups.
  • Negative acute phase protein, transferrin, showed a positive response in the starch group and starch and buffer group suggesting a correlation with the onset of the disease.
  • Ceruloplasmin, another protein, was observed to have a positive correlation with the severity of laminitis symptoms in the starch and buffer group.
  • The concentration of ceruloplasmin, α-1-antitrypsin, and haptoglobin increased in the starch and buffer group, indicating an inflammatory response in these horses.

Implication of Findings

  • The findings suggest carbohydrate overload can induce laminitis in horses possibly due to inflammatory responses. This information provides valuable insight into the causes of the condition could be linked to diet.
  • The study also demonstrated that a buffering solution doesn’t provide prevention from this condition, contradicting previous belief.
  • The observed correlations and levels of specific proteins following the onset of the disease suggest potential biomarkers for detecting or monitoring the progress of laminitis, leading to improved means of prediction and treatment planning.

Cite This Article

APA
Peixoto Rabelo I, Barroco de Paula V, Carvalho Bustamante C, Santana AM, Gomes da Silva D, Baldassi AC, Canola PA, Araújo Valadão CA. (2023). Acute phase proteins levels in horses, after a single carbohydrate overload, associated with cecal alkalinization. Front Vet Sci, 10, 1043656. https://doi.org/10.3389/fvets.2023.1043656

Publication

Frontiers in veterinary science
ISSN: 2297-1769
NlmUniqueID: 101666658
Country: Switzerland
Language: English
Volume: 10
Pages: 1043656
PII: 1043656

Researcher Affiliations

Peixoto Rabelo, Isabela
  • Department of Veterinary Internal Medicine and Surgery, School of Agricultural and Veterinarian Sciences, São Paulo State University (Unesp), Jaboticabal, São Paulo, Brazil.
Barroco de Paula, Vanessa
  • Department of Veterinary Internal Medicine and Surgery, School of Agricultural and Veterinarian Sciences, São Paulo State University (Unesp), Jaboticabal, São Paulo, Brazil.
Carvalho Bustamante, Caio
  • Department of Veterinary Internal Medicine and Surgery, School of Agricultural and Veterinarian Sciences, São Paulo State University (Unesp), Jaboticabal, São Paulo, Brazil.
Santana, André Marcos
  • Department of Veterinary Medicine, Maringá State University, Umuarama, Paraná, Brazil.
Gomes da Silva, Daniela
  • Department of Veterinary Internal Medicine and Surgery, School of Agricultural and Veterinarian Sciences, São Paulo State University (Unesp), Jaboticabal, São Paulo, Brazil.
Baldassi, Amanda Cristina
  • Department of Agricultural, Livestock and Environmental Biotechnology, School of Agricultural and Veterinarian Sciences, São Paulo State University (Unesp), Jaboticabal, São Paulo, Brazil.
Canola, Paulo Aléscio
  • Department of Veterinary Internal Medicine and Surgery, School of Agricultural and Veterinarian Sciences, São Paulo State University (Unesp), Jaboticabal, São Paulo, Brazil.
Araújo Valadão, Carlos Augusto
  • Department of Veterinary Internal Medicine and Surgery, School of Agricultural and Veterinarian Sciences, São Paulo State University (Unesp), Jaboticabal, São Paulo, Brazil.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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