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Home / Equine Research Bank / Andrologia / Addition of caffeine to equine thawed sperm increases motili...
Andrologia2020; 53(2); e13918; doi: 10.1111/and.13918

Addition of caffeine to equine thawed sperm increases motility and decreases nitrite concentration.

Abstract: The aim of this study was to improve the quality of frozen-thawed equine sperm by the addition of caffeine to it. Semen from nine stallions was frozen and different concentrations of caffeine (3, 5 and 7.5 mM) were added to frozen-thawed semen. The sperm kinetic parameters, membrane functionality and integrity, and acrosome integrity and spontaneous acrosome reacted sperm were evaluated with a computer-assisted sperm analysis, a hypoosmotic swelling test and epifluorescent microscopy, respectively. Nitrite and hydroperoxide concentrations of frozen-thawed semen were measured using spectrophotometry. Sperm fertility was evaluated by artificial insemination (AI) of 16 mares with thawed ejaculates (control and 5 mM caffeine-treated groups). Compared to that in the control, the addition of 5 mM caffeine induced an increase in sperm motility (38.9 ± 2.8 versus 32.6 ± 3.4%), and a decrease in nitrite concentration (11.4 ± 2.1 versus 12.8 ± 2.9 µM/µg protein, p < .05). Moreover, the pregnancy rate from AI in the caffeine group was significantly higher (62.5%) than that in the control group (12.5%). These data suggest that caffeine reduced the nitrite concentration and enhanced sperm motility in thawed equine sperm, thus increasing the fertility rate in mares inseminated with caffeine-treated equine semen.
© 2020 Wiley-VCH GmbH.
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Publication Date: 2020-12-28 PubMed ID: 33368488DOI: 10.1111/and.13918Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research was conducted to investigate the influence of adding caffeine to frozen-thawed horse sperm on its quality. It was found that the inclusion of caffeine enhanced sperm motility and simultaneously reduced nitrite concentration, resulting in a significantly increased fertility rate.

Methods of the Study

  • The experiment involved the semen sample from nine stallions, which was frozen for the study.
  • Different caffeine concentrations of 3, 5, and 7.5 mM were each added to the frozen-thawed semen to observe the changes.

Testing and Evaluation Procedures

  • The sperm’s kinetic parameters, membrane functionality and integrity, and acrosome integrity were evaluated through computer-assisted sperm analysis, a hypoosmotic swelling test, and epifluorescent microscopy respectively.
  • Nitrite and hydroperoxide concentrations in the frozen-thawed semen were measured using spectrophotometry.
  • Sperm fertility was further evaluated by artificially inseminating 16 mares using the thawed ejaculates in both the control group and the group treated with 5 mM caffeine.

Results of the Research

  • The inclusion of a 5 mM caffeine concentration resulted in enhanced sperm motility (38.9 ± 2.8 compared to 32.6 ± 3.4% in the control group).
  • The nitrite concentration was also reduced in comparison to the control group (11.4 ± 2.1 versus 12.8 ± 2.9 µM/µg protein).
  • The pregnancy rate from artificial insemination in the caffeine group was considerably higher at 62.5% as compared to 12.5% in the control group.

Conclusion of the Research

Through this study, it is suggested that caffeine plays a significant role in enhancing the quality of thawed horse sperm. This study implies a positive change in sperm motility and a decrease in nitrite concentrations, resulting in higher fertility rates. This study can lead to more efficient and successful artificial insemination procedures in horses, ultimately improving breeding practices.

Cite This Article

APA
Alves NC, Diniz SA, Viegas RN, Cortes SF, Costa ED, Freitas MM, Martins-Filho OA, Araújo MSS, Lana ÂMQ, Wenceslau RR, Lagares MA. (2020). Addition of caffeine to equine thawed sperm increases motility and decreases nitrite concentration. Andrologia, 53(2), e13918. https://doi.org/10.1111/and.13918

Publication

Andrologia
ISSN: 1439-0272
NlmUniqueID: 0423506
Country: Germany
Language: English
Volume: 53
Issue: 2
Pages: e13918

Researcher Affiliations

Alves, Natália de Castro
  • Veterinary School, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil.
Diniz, Soraia de Araújo
  • Veterinary School, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil.
Viegas, Rodrigo Novaes
  • Veterinary School, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil.
Cortes, Steyner Franca
  • Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil.
Costa, Eduardo Damasceno
  • Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil.
Freitas, Marina Morra
  • Faculty of Veterinary Medicine, Pontifical Catholic University of Minas Gerais, Betim, Brazil.
Martins-Filho, Olindo Assis
  • René Rachou Research Center - Fiocruz, Laboratory of Diagnostic and Monitoring Biomarkers, Belo Horizonte, Brazil.
Araújo, Márcio Sobreira Silva
  • René Rachou Research Center - Fiocruz, Laboratory of Diagnostic and Monitoring Biomarkers, Belo Horizonte, Brazil.
Lana, Ângela Maria Quintão
  • Veterinary School, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil.
Wenceslau, Raphael Rocha
  • Veterinary School, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil.
Lagares, Monique de Albuquerque
  • Veterinary School, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil.

MeSH Terms

  • Animals
  • Caffeine / pharmacology
  • Cryopreservation
  • Female
  • Horses
  • Humans
  • Insemination, Artificial / veterinary
  • Male
  • Nitrites
  • Pregnancy
  • Semen Preservation / veterinary
  • Sperm Motility
  • Spermatozoa

Grant Funding

  • 1690817/2017-0487 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

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