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Home / Equine Research Bank / Reprod Domest Anim / Addition of seminal plasma to post-thawing equine semen: wha...
Reproduction in domestic animals = Zuchthygiene2010; 46(4); 682-686; doi: 10.1111/j.1439-0531.2010.01729.x

Addition of seminal plasma to post-thawing equine semen: what is the effect on sperm cell viability?

Abstract: Effect of seminal plasma addition after thawing on viability or cryocapacitation is not definitively established. This experiment was performed to verify the effect of adding seminal plasma, autologous or homologous (from an animal with good semen freezability). Five ejaculates from each of four stallions with proven fertility were collected and cryopreserved. The semen was subsequently thawed and divided into the following three treatment groups: no seminal plasma addition after semen thawing (NOSP); the addition of homologous seminal plasma after semen thawing (HSP) and the addition of autologous seminal plasma after semen thawing (ASP). The addition of 20% of seminal plasma led to an increase in the cell population that simultaneously show plasma and acrosomal membrane integrity (p < 0.05). The addition of seminal plasma did not alter the total motility, the amount of cells with mitochondrial membrane potential or the sperm velocities (average path velocity, straight-line velocity and curvilinear velocity). However, the beat/cross-frequency, straightness and linearity were reduced in ASP and HSP groups (p < 0.05). Unexpectedly, the addition of homologous seminal plasma reduced the proportion of cells with progressive motility (p < 0.05) and the addition of autologous seminal plasma reduced the amplitude of the lateral head displacement (p < 0.05). Based on the increase in the cell populations that had the plasma and acrosomal membrane integrity simultaneously identified in this study, we proposed that the addition of seminal plasma (autologous or homologous) into post-thawed semen before insemination could increase semen fertility.
© 2010 Blackwell Verlag GmbH.
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Publication Date: 2010-12-01 PubMed ID: 21121969DOI: 10.1111/j.1439-0531.2010.01729.xGoogle Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research studied the effects of adding seminal plasma after thawing horse semen on sperm cell viability. Findings suggest that adding seminal plasma (autologous or homologous) can boost semen fertility by increasing the population of cells showing plasma and acrosomal membrane integrity.

Objective and Methodology

  • The study aimed to establish the effects of adding seminal plasma to horse semen after it has been frozen and then thawed.
  • A total of five ejaculates were collected from each of four stallions known to be fertile and then cryopreserved (frozen).
  • The semen was divided into three groups for treatment: no seminal plasma addition (NOSP), addition of homologous seminal plasma (from a different healthy animal – HSP), and addition of autologous seminal plasma (from the same animal – ASP).

Findings

  • The addition of 20% seminal plasma led to an increase in the cell population that displayed both plasma and acrosomal membrane integrity.
  • The study found no effect on the total motility, the number of cells with mitochondrial membrane potential, or the sperm’s velocities.
  • However, beat/cross-frequency, straightness, and linearity were reduced in the ASP and HSP groups.
  • Unexpectedly, the addition of homologous seminal plasma reduced the proportion of cells with progressive motility and addition of autologous seminal plasma reduced the amplitude of the lateral head displacement.

Conclusion and Implications

  • Considering the increase in the cell populations that had both acrosomal and plasma membrane integrity, it is proposed that adding seminal plasma (autologous or homologous) to thawed semen before insemination could possibly enhance the fertility of the semen.
  • This could potentially be a significant finding for the field of equine reproduction management, possibly improving the outcomes of artificial insemination procedures.

Cite This Article

APA
de Andrade AF, Zaffalon FG, Celeghini EC, Nascimento J, Tarragó OF, Martins SM, Alonso MA, Arruda RP. (2010). Addition of seminal plasma to post-thawing equine semen: what is the effect on sperm cell viability? Reprod Domest Anim, 46(4), 682-686. https://doi.org/10.1111/j.1439-0531.2010.01729.x

Publication

Reproduction in domestic animals = Zuchthygiene
ISSN: 1439-0531
NlmUniqueID: 9015668
Country: Germany
Language: English
Volume: 46
Issue: 4
Pages: 682-686

Researcher Affiliations

de Andrade, A F C
  • Laboratory of Semen Biotechnology and Andrology, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo (USP), Pirassununga, Brazil.
Zaffalon, F G
    Celeghini, E C C
      Nascimento, J
        Tarragó, O F B
          Martins, S M M K
            Alonso, M A
              Arruda, R P

                MeSH Terms

                • Animals
                • Cryopreservation / veterinary
                • Horses / physiology
                • Insemination, Artificial / veterinary
                • Male
                • Semen / physiology
                • Semen Preservation / veterinary
                • Sperm Motility / physiology
                • Spermatozoa / physiology

                Citations

                This article has been cited 7 times.
                1. Zambrano F, Pezo F, Furugen Cesar de Andrade A, Rivera-Concha R, Uribe P, Schulz M, Zapparoli H, Mendes de Oliveira Bezerra L, Hermosilla C, Taubert A, Sánchez R. Modulation of NETosis in Swine Neutrophil-Spermatozoa Co-Cultures In Vitro: Effects of Butylated Hydroxytoluene, Albumin, Prostaglandin E(2), and Seminal Plasma. Antioxidants (Basel) 2025 Jun 24;14(7).
                  doi: 10.3390/antiox14070778pubmed: 40722882google scholar: lookup
                2. Egyptien S, Deleuze S, Ledeck J, Ponthier J. Sperm Quality Assessment in Stallions: How to Choose Relevant Assays to Answer Clinical Questions. Animals (Basel) 2023 Oct 6;13(19).
                  doi: 10.3390/ani13193123pubmed: 37835729google scholar: lookup
                3. Seyedasgari F, Asadi B, Kim E. Seminal plasma modulates post-thaw longevity and motility of frozen sperm in dromedary camel. Anim Biosci 2023 Dec;36(12):1821-1830.
                  doi: 10.5713/ab.23.0136pubmed: 37641835google scholar: lookup
                4. Xu B, Bai X, Zhang J, Li B, Zhang Y, Su R, Wang R, Wang Z, Lv Q, Zhang J, Li J. Metabolomic analysis of seminal plasma to identify goat semen freezability markers. Front Vet Sci 2023;10:1132373.
                  doi: 10.3389/fvets.2023.1132373pubmed: 36968471google scholar: lookup
                5. Bubenickova F, Postlerova P, Simonik O, Sirohi J, Sichtar J. Effect of Seminal Plasma Protein Fractions on Stallion Sperm Cryopreservation. Int J Mol Sci 2020 Sep 3;21(17).
                  doi: 10.3390/ijms21176415pubmed: 32899253google scholar: lookup
                6. Šichtař J, Bubeníčková F, Sirohi J, Šimoník O. How to Increase Post-Thaw Semen Quality in Poor Freezing Stallions: Preliminary Results of the Promising Role of Seminal Plasma Added after Thawing. Animals (Basel) 2019 Jul 3;9(7).
                  doi: 10.3390/ani9070414pubmed: 31277261google scholar: lookup
                7. Torres MA, Díaz R, Boguen R, Martins SM, Ravagnani GM, Leal DF, Oliveira Mde L, Muro BB, Parra BM, Meirelles FV, Papa FO, Dell'Aqua JA Jr, Alvarenga MA, Moretti Ade S, Sepúlveda N, de Andrade AF. Novel Flow Cytometry Analyses of Boar Sperm Viability: Can the Addition of Whole Sperm-Rich Fraction Seminal Plasma to Frozen-Thawed Boar Sperm Affect It?. PLoS One 2016;11(8):e0160988.
                  doi: 10.1371/journal.pone.0160988pubmed: 27529819google scholar: lookup
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