Adipogenic differentiation of adult equine mesenchymal stromal cells.
Abstract: Equine adipose tissue-derived mesenchymal stem cells (ASCs) have only recently been investigated for their adipogenic, chondrogenic, and osteogenic differentiation potential. This chapter will briefly outline the molecular mechanisms leading to adipogenesis and the methods of equine adipose tissue harvest, ASC isolation, and adipogenic differentiation. The reader is also directed to other reported methods of adipogenesis for ASCs and mesenchymal stem cells (MSCs) from other tissues.
Publication Date: 2010-11-18 PubMed ID: 21082395DOI: 10.1007/978-1-61737-960-4_6Google Scholar: Lookup
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- Journal Article
Summary
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This research article investigates the potential for fat tissue-derived stem cells from horses to differentiate and develop into fat, cartilage, and bone cells. The authors also provide an overview of the methods used for harvesting horse adipose tissue and isolating and differentiating the stem cells.
Overview of Molecular Mechanisms
- The research begins with an outline of the molecular mechanisms leading to adipogenesis, which is the process of cell differentiation where pre-adipocytes transform into adipocytes or fat cells.
- Understanding these mechanisms is critical for dissecting the biological processes and factors influencing the differentiation of adipose tissue-derived mesenchymal stem cells (ASCs).
Harvesting Equine Adipose Tissue
- One key aspect of the study involves explaining the methods for harvesting equine adipose tissue.
- This process is crucial to obtaining the primary materials (the ASCs) required for the study.
- Details would likely include the procedural steps, any tissue preparation, and potential challenges faced during the tissue harvest.
ASC Isolation and Adipogenic Differentiation
- Another critical section of this research discusses the isolation of ASCs from harvested equine adipose tissue as well as the process of initiating adipogenic differentiation.
- This section could highlight the methodologies implemented to isolate these stem cells and the techniques used to induce their differentiation into adipocytes.
Comparison with Other Methods
- The authors also elaborate on other reported methods of ASC adipogenesis.
- This comparison is likely to highlight their chosen methodology’s advantages and disadvantages over different methods and practices.
- Such analysis can provide context and help identify potential improvements or alternatives to the current methods.
Applications Beyond Equine ASCs
- While the primary focus is on equine ASCs, the authors also consider the applicability of their findings and methodologies to mesenchymal stem cells (MSCs) extracted from other tissues or other animal species.
- This extension of their research could benefit the broader scientific community by offering insights applicable to various research scenarios.
Cite This Article
APA
Vidal MA, Lopez MJ.
(2010).
Adipogenic differentiation of adult equine mesenchymal stromal cells.
Methods Mol Biol, 702, 61-75.
https://doi.org/10.1007/978-1-61737-960-4_6 Publication
Researcher Affiliations
- JD Wheat Veterinary Orthopedic Research Laboratory, School of Veterinary Medicine, University of California - Davis, Davis, CA, USA. mavidal@ucdavis.edu
MeSH Terms
- Adipogenesis
- Aging / physiology
- Animals
- Cell Culture Techniques / methods
- Cell Separation
- Horses
- Mesenchymal Stem Cells / cytology
- Staining and Labeling
- Stromal Cells / cytology
Citations
This article has been cited 2 times.- Yang Q, Lopez MJ. Ultrastructural morphology is distinct among primary progenitor cell isolates from normal, inflamed, and cryopreserved equine hoof tissue and CD105(+)K14(+) progenitor cells.. In Vitro Cell Dev Biol Anim 2019 Sep;55(8):641-655.
- Menssen A, Häupl T, Sittinger M, Delorme B, Charbord P, Ringe J. Differential gene expression profiling of human bone marrow-derived mesenchymal stem cells during adipogenic development.. BMC Genomics 2011 Sep 24;12:461.
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