Advances in developing molecular-diagnostic tools for strongyloid nematodes of equids: fundamental and applied implications.
Abstract: Infections of equids with parasitic nematodes of the order Strongylida (subfamilies Strongylinae and Cyathostominae) are of major veterinary importance. In last decades, the widespread use of drugs against these parasites has led to problems of resistance within the Cyathostominae, and to an increase in their prevalence and intensity of infection. Novel control strategies, based on improved knowledge of parasite biology and epidemiology, have thus become important. However, there are substantial limitations in the understanding of fundamental biological and systematic aspects of these parasites, which have been due largely to limitations in their specific identification and diagnosis using traditional, morphological approaches. Recently, there has been progress in the development of DNA-based approaches for the specific identification of strongyloids of equids for systematic studies and disease diagnosis. The present article briefly reviews information on the classification, biology, pathogenesis, epidemiology of equine strongyloids and the diagnosis of infections, highlights knowledge gaps in these areas, describes recent advances in the use of molecular techniques for the genetic characterisation, specific identification and differentiation of strongyloids of equids as a basis for fundamental investigations of the systematics, population biology and ecology.
Publication Date: 2004-03-24 PubMed ID: 15036364DOI: 10.1016/j.mcp.2003.10.001Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
- Review
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research investigates the application of DNA-based approaches for specific identification and diagnosis of strongyloid nematodes in equids. It reviews current knowledge and recent advances in the field and explores the use of these molecular techniques to improve understanding of the biology, systematics, and ecology of strongyloid nematodes.
Context and Objective
- This research was conducted in light of prevalent issues with parasitic nematodes of the order Strongylida, subfamilies Strongylinae and Cyathostominae, in equids (horses, donkeys, and zebras). The indiscriminate use of drugs to control these parasites has led to resistance and a rise in their prevalence and intensity of infection.
- The researchers identified a need for more effective control strategies, based on improved knowledge of parasite biology and epidemiology. They also pinpointed a gap in the understanding of fundamental biological and systematic aspects of these parasites due to the limitations of traditional, morphological approaches used for their identification and diagnosis.
- The primary objective, therefore, was to explore the potential of DNA-based approaches for the specific identification of equid strongyloids, which could lead to a more comprehensive understanding of their biology, systematics, and epidemiology.
Strategy and Methods
- The researchers reviewed existing information on the classification, biology, pathogenesis, and epidemiology of equine strongyloids as well as the methods for diagnosing infections.
- They pointed out the knowledge gaps in these areas and highlighted the value and practical implications of using molecular techniques for these purposes.
- In particular, they explored the use of molecular-genetic characterisation for the specific identification and differentiation of equid strongyloids. This strategy offers a basis for systematic studies and disease diagnosis, opening up the potential for greater insight into the systematics, population biology, and ecology of these parasites.
Implication and Conclusion
- The development and application of DNA-based techniques for identifying and diagnosing infections of strongyloid nematodes in equids could significantly improve control strategies against these harmful parasites. By offering a more precise method than traditional morphological approaches, it could lead to a more nuanced understanding of the biology and ecology of these parasites.
- This could, in turn, create the potential for better informed, more effective interventions to manage and control these parasites, addressing current issues of drug resistance and prevalence of infections.
Cite This Article
APA
Gasser RB, Hung GC, Chilton NB, Beveridge I.
(2004).
Advances in developing molecular-diagnostic tools for strongyloid nematodes of equids: fundamental and applied implications.
Mol Cell Probes, 18(1), 3-16.
https://doi.org/10.1016/j.mcp.2003.10.001 Publication
Researcher Affiliations
- Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia. robinbg@unimelb.edu.au
MeSH Terms
- Animals
- DNA, Helminth
- DNA, Ribosomal / analysis
- Equidae / parasitology
- Molecular Diagnostic Techniques / methods
- Parasitic Diseases, Animal / diagnosis
- Strongylida / classification
- Strongylida / genetics
- Strongylida Infections / diagnosis
- Strongylida Infections / epidemiology
- Strongylida Infections / etiology
- Strongylida Infections / veterinary
Citations
This article has been cited 14 times.- Sangwalee W, Norkaew J, Inthachak S, Janwan P, Rodpai R, Sanpool O, Sadaow L, Boonroumkaew P, Intapan PM, Maleewong W, Thanchomnang T. Prevalence of intestinal parasitic infections and genetic differentiation of Strongyloides stercoralis among migrant workers from Myanmar, Lao PDR and Cambodia in northeastern Thailand. PLoS One 2022;17(12):e0279754.
- El-Gameel SM, Al-Mokaddem AK, Salaeh NMK, Attia MM. Morphomolecular characterization of Strongylus vulgaris isolated from donkeys with special references to histopathological study on the affected organs. J Parasit Dis 2022 Sep;46(3):795-803.
- Tombak KJ, Hansen CB, Kinsella JM, Pansu J, Pringle RM, Rubenstein DI. The gastrointestinal nematodes of plains and Grevy's zebras: Phylogenetic relationships and host specificity. Int J Parasitol Parasites Wildl 2021 Dec;16:228-235.
- Elmeligy E, Abdelbaset A, Elsayed HK, Bayomi SA, Hafez A, Abu-Seida AM, El-Khabaz KAS, Hassan D, Ghandour RA, Khalphallah A. Oxidative stress in Strongylus spp. infected donkeys treated with piperazine citrate versus doramectin. Open Vet J 2021 Apr-Jun;11(2):238-250.
- Louro M, Kuzmina TA, Bredtmann CM, Diekmann I, de Carvalho LMM, von Samson-Himmelstjerna G, Krücken J. Genetic variability, cryptic species and phylogenetic relationship of six cyathostomin species based on mitochondrial and nuclear sequences. Sci Rep 2021 Apr 15;11(1):8245.
- Lindroth KM, Dicksved J, Pelve E, Båverud V, Müller CE. Faecal bacterial composition in horses with and without free faecal liquid: a case control study. Sci Rep 2021 Feb 26;11(1):4745.
- Bellaw JL, Nielsen MK. Meta-analysis of cyathostomin species-specific prevalence and relative abundance in domestic horses from 1975-2020: emphasis on geographical region and specimen collection method. Parasit Vectors 2020 Oct 12;13(1):509.
- Saeed MA, Beveridge I, Abbas G, Beasley A, Bauquier J, Wilkes E, Jacobson C, Hughes KJ, El-Hage C, O'Handley R, Hurley J, Cudmore L, Carrigan P, Walter L, Tennent-Brown B, Nielsen MK, Jabbar A. Systematic review of gastrointestinal nematodes of horses from Australia. Parasit Vectors 2019 Apr 29;12(1):188.
- Meshgi B, Jalousian F, Masih Z. Phylogenetic Study of Haemonchus Species from Iran Based On Morpho-Molecular Characterization. Iran J Parasitol 2015 Apr-Jun;10(2):189-96.
- Das MK, Dhamodharan R, Hoti SL, Dash AP. Molecular differentiation of nocturnally periodic and diurnally sub-periodic Wuchereria bancrofti by Randomly Amplified Polymorphic DNA (RAPD). World J Microbiol Biotechnol 2011 Jun;27(6):1525-30.
- Lyons ET, Kuzmina TA, Tolliver SC, Collins SS. Observations on development of natural infection and species composition of small strongyles in young equids in Kentucky. Parasitol Res 2011 Dec;109(6):1529-35.
- Kharchenko V, Kuzmina T, Trawford A, Getachew M, Feseha G. Morphology and diagnosis of some fourth-stage larvae of cyathostomines (Nematoda: Strongyloidea) in donkeys Equus asinus L. from Ethiopia. Syst Parasitol 2009 Jan;72(1):1-13.
- Traversa D, Iorio R, Klei TR, Kharchenko VA, Gawor J, Otranto D, Sparagano OA. New method for simultaneous species-specific identification of equine strongyles (nematoda, strongylida) by reverse line blot hybridization. J Clin Microbiol 2007 Sep;45(9):2937-42.
- Klass LG, Krücken J, Mbedi S, Sparmann S, Schenk T, Andreotti S, von Samson-Himmelstjerna G. Characterizing mixed strongyle infections in foals and broodmares using cytochrome c oxidase subunit I deep amplicon sequencing. Parasit Vectors 2026 Jan 3;19(1):65.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
