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Home / Equine Research Bank / J Hyg (Lond) / Aeromonas hydrophila in livestock: incidence, biochemical ch...
The Journal of hygiene1984; 92(3); 365-375; doi: 10.1017/s0022172400064585

Aeromonas hydrophila in livestock: incidence, biochemical characteristics and antibiotic susceptibility.

Abstract: Faecal samples from 110 horses, 115 pigs, 111 sheep and 123 cows were examined for the presence of Aeromonas hydrophila, which was also sought in the available drinking water. The overall faecal rate was 11.8%, but significantly more bovine than other samples were found to be positive. There was significant association between the isolation of A. hydrophila from all animal faeces and its presence in drinking water, but this was not found when individual animal groups were analysed separately. An enrichment technique increased the total number of isolates by 77.1%. Strains of differing origins could not be differentiated by biotyping, although fermentation of sorbitol was associated with bovine isolates. There was a strong positive correlation between positive reactions for V--P, gluconate oxidase and haemolysis of rabbit erythrocytes, tests which had previously been shown to correlate with production of enterotoxin and cytotoxin. Biotypes giving positive reactions for these tests were most frequently isolated from cows, sheep and untreated water, and less frequently from pigs and horses. Most strains of A. hydrophila were resistant to amoxycillin, carbenicillin and cephradine, and sensitive to gentamicin, chloramphenicol and neomycin.
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Publication Date: 1984-06-01 PubMed ID: 6736644PubMed Central: PMC2129318DOI: 10.1017/s0022172400064585Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article investigates the occurrence, biochemical properties, and antibiotic susceptibility of the bacterium Aeromonas hydrophila in livestock and their drinking water. It was revealed that the bacteria were present in the feces and water of all tested species but was significantly higher in cows. The detection of the bacteria in the animal feces was found to be strongly correlated with its presence in the drinking water.

Methodology and Findings

  • The study analyzed fecal samples from 110 horses, 115 pigs, 111 sheep, and 123 cows, as well as their drinking water.
  • The overall detection rate of A. hydrophila in the fecal samples was found to be 11.8%. However, the occurrence was significantly higher in bovine samples than in others.
  • The study found a significant association between the presence of A. hydrophila in the feces of all the animals and its presence in their drinking water.
  • An enrichment technique developed for the study increased the total number of bacteria isolates by 77.1%.

Biotyping and Antimicrobial Resistance

  • The dissimilar origins of A. hydrophila samples couldn’t be differentiated based on biotyping.
  • However, the practice of sorbitol fermentation was associated mostly with bovine isolates.
  • “V–P,” “gluconate oxidase,” and “rabbit erythrocyte haemolysis” reactions were positively correlated with A. hydrophila strains. These tests have previously been proven to be connected with the bacteria’s ability to produce enterotoxin and cytotoxin.
  • The most common sources of these biotypes were cows, sheep, and untreated water, while pigs and horses were less common sources.
  • The majority of A. hydrophila strains were resistant to amoxicillin, carbenicillin, and cephradine, but sensitive to gentamicin, chloramphenicol, and neomycin.

Implications

  • The research findings have significant implications for livestock health, as the bacteria could potentially pose a risk to the animals, particularly bovines.
  • The study also suggests a potential public health risk through the consumption of contaminated meat or dairy products, or contact with infected livestock or their waste.
  • The bacterial resistance to some common antibiotics observed in the study further underlines these concerns, making the findings relevant to veterinarians, farmers, public health officials and others involved in livestock production.

Cite This Article

APA
Gray SJ. (1984). Aeromonas hydrophila in livestock: incidence, biochemical characteristics and antibiotic susceptibility. J Hyg (Lond), 92(3), 365-375. https://doi.org/10.1017/s0022172400064585

Publication

The Journal of hygiene
ISSN: 0022-1724
NlmUniqueID: 0375374
Country: England
Language: English
Volume: 92
Issue: 3
Pages: 365-375

Researcher Affiliations

Gray, S J

    MeSH Terms

    • Aeromonas / classification
    • Aeromonas / drug effects
    • Aeromonas / isolation & purification
    • Animals
    • Animals, Domestic / microbiology
    • Anti-Bacterial Agents / pharmacology
    • Artiodactyla / microbiology
    • Bacterial Toxins / biosynthesis
    • Carbohydrate Metabolism
    • Cattle / microbiology
    • Enterotoxins / biosynthesis
    • Feces / microbiology
    • Horses / microbiology
    • Microbial Sensitivity Tests
    • Sheep / microbiology
    • Swine / microbiology
    • Water Microbiology
    • Water Supply

    References

    This article includes 30 references
    1. Von Graevenitz A, Mensch AH. The genus aeromonas in human bacteriology; report of 30 cases and review of the literature.. N Engl J Med 1968 Feb 1;278(5):245-9.
      pubmed: 5635458doi: 10.1056/NEJM196802012780504google scholar: lookup
    2. von Graevenitz A, Zinterhofer L. The detection of Aeromonas hydrophila in stool specimens.. Health Lab Sci 1970 Jul;7(3):124-7.
      pubmed: 4915588
    3. Wohlgemuth K, Pierce RL, Kirkbride CA. Bovine abortion associated with Aeromonas hydrophila.. J Am Vet Med Assoc 1972 Apr 1;160(7):1001-2.
      pubmed: 5014883
    4. Shotts EB Jr, Gaines JL Jr, Martin L, Prestwood AK. Aeromonas-induced deaths among fish and reptiles in an eutrophic inland lake.. J Am Vet Med Assoc 1972 Sep 15;161(6):603-7.
      pubmed: 5056185
    5. Marcus LC. Infectious diseases of reptiles.. J Am Vet Med Assoc 1971 Dec 1;159(11):1626-31.
      pubmed: 4949438
    6. Ketover BP, Young LS, Armstrong D. Septicemia due to Aeromonas hydrophila: clinical and immunologic aspects.. J Infect Dis 1973 Mar;127(3):284-90.
      pubmed: 4688925doi: 10.1093/infdis/127.3.284google scholar: lookup
    7. Chatterjee BD, Neogy KN. Studies on Aeromonas and Plesiomonas species isolated from cases of choleraic diarrhoea.. Indian J Med Res 1972 Apr;60(4):520-4.
      pubmed: 4660283
    8. Phillips JA, Bernhardt HE, Rosenthal SG. Aeromonas hydrophila infections.. Pediatrics 1974 Jan;53(1):110-2.
      pubmed: 4809188
    9. Bhat P, Shanthakumari S, Rajan D. The characterization and significance of Plesiomonas shigelloides and Aeromonas hydrophila isolated from an epidemic of diarrhoea.. Indian J Med Res 1974 Jul;62(7):1051-60.
      pubmed: 4435916
    10. Sanyal SC, Singh SJ, Sen PC. Enteropathogenicity of Aeromonas hydrophila and Plesiomonas shigelloides.. J Med Microbiol 1975 Feb;8(1):195-8.
      pubmed: 1092866doi: 10.1099/00222615-8-1-195google scholar: lookup
    11. Hanson PG, Standridge J, Jarrett F, Maki DG. Freshwater wound infection due to Aeromonas hydrophila.. JAMA 1977 Sep 5;238(10):1053-4.
      pubmed: 577949
    12. Ljungh A, Popoff M, Wadstrom T. Aeromonas hydrophila in acute diarrheal disease: detection of enterotoxin and biotyping of strains.. J Clin Microbiol 1977 Aug;6(2):96-100.
      pubmed: 893660doi: 10.1128/jcm.6.2.96-100.1977google scholar: lookup
    13. Annapurna E, Sanyal SC. Enterotoxicity of Aeromonas hydrophila.. J Med Microbiol 1977 Aug;10(3):317-23.
      pubmed: 894697doi: 10.1099/00222615-10-3-317google scholar: lookup
    14. Davis WA 2nd, Kane JG, Garagusi VF. Human aeromonas infections: a review of the literature and a case report of endocarditis.. Medicine (Baltimore) 1978 May;57(3):267-77.
      pubmed: 347223
    15. Fulghum DD, Linton WR, Taplin D. Fatal Aeromonas hydrophila infection of the skin.. South Med J 1978 Jun;71(6):739-41.
      pubmed: 663710doi: 10.1097/00007611-197806000-00037google scholar: lookup
    16. Trust TJ, Chipman DC. Clinical involvement of Aeromonas hydrophila.. Can Med Assoc J 1979 Apr 21;120(8):942-6.
      pubmed: 373876
    17. Cumberbatch N, Gurwith MJ, Langston C, Sack RB, Brunton JL. Cytotoxic enterotoxin produced by Aeromonas hydrophila: relationship of toxigenic isolates to diarrheal disease.. Infect Immun 1979 Mar;23(3):829-37.
      pubmed: 457260doi: 10.1128/iai.23.3.829-837.1979google scholar: lookup
    18. Dubey RS, Sanyal SC. Characterisation and neutralisation of Aeromonas hydrophila enterotoxin in the rabbit ileal-loop model.. J Med Microbiol 1979 Aug;12(3):347-54.
      pubmed: 38341doi: 10.1099/00222615-12-3-347google scholar: lookup
    19. Daily OP, Joseph SW, Coolbaugh JC, Walker RI, Merrell BR, Rollins DM, Seidler RJ, Colwell RR, Lissner CR. Association of Aeromonas sobria with human infection.. J Clin Microbiol 1981 Apr;13(4):769-77.
      pubmed: 6112237doi: 10.1128/jcm.13.4.769-777.1981google scholar: lookup
    20. Kaper JB, Lockman H, Colwell RR, Joseph SW. Aeromonas hydrophila: ecology and toxigenicity of isolates from an estuary.. J Appl Bacteriol 1981 Apr;50(2):359-77.
      pubmed: 7251516doi: 10.1111/j.1365-2672.1981.tb00900.xgoogle scholar: lookup
    21. Ampel N, Peter G. Aeromonas bacteraemia in a burn patient.. Lancet 1981 Oct 31;2(8253):987.
      pubmed: 6117752doi: 10.1016/s0140-6736(81)91183-1google scholar: lookup
    22. Cookson BD, Houang EC, Lee JV. Clustering of aeromonas hydrophila septicaemia.. Lancet 1981 Nov 28;2(8257):1232.
      pubmed: 6118660doi: 10.1016/s0140-6736(81)91472-0google scholar: lookup
    23. Gracey M, Burke V, Rockhill RC, Suharyono, Sunoto. Aeromonas species as enteric pathogens.. Lancet 1982 Jan 23;1(8265):223-4.
      pubmed: 6119581doi: 10.1016/s0140-6736(82)90787-5google scholar: lookup
    24. Pitarangsi C, Echeverria P, Whitmire R, Tirapat C, Formal S, Dammin GJ, Tingtalapong M. Enteropathogenicity of Aeromonas hydrophila and Plesiomonas shigelloides: prevalence among individuals with and without diarrhea in Thailand.. Infect Immun 1982 Feb;35(2):666-73.
      pubmed: 7056580doi: 10.1128/iai.35.2.666-673.1982google scholar: lookup
    25. Ljungh A, Wretlind B, Möllby R. Separation and characterization of enterotoxin and two haemolysins from Aeromonas hydrophila.. Acta Pathol Microbiol Scand B 1981 Dec;89(6):387-97.
      pubmed: 7336925doi: 10.1111/j.1699-0463.1981.tb00205_89b.xgoogle scholar: lookup
    26. Moulsdale MT. Isolation of Aeromonas from faeces.. Lancet 1983 Feb 12;1(8320):351.
      pubmed: 6130348doi: 10.1016/s0140-6736(83)91649-5google scholar: lookup
    27. von Graevenitz A, Bucher C. Evaluation of differential and selective media for isolation of Aeromonas and Plesiomonas spp. from human feces.. J Clin Microbiol 1983 Jan;17(1):16-21.
      pubmed: 6826700doi: 10.1128/jcm.17.1.16-21.1983google scholar: lookup
    28. Burke V, Robinson J, Atkinson HM, Gracey M. Biochemical characteristics of enterotoxigenic Aeromonas spp.. J Clin Microbiol 1982 Jan;15(1):48-52.
      pubmed: 6764776doi: 10.1128/jcm.15.1.48-52.1982google scholar: lookup
    29. SHAW C, CLARKE PH. Biochemical classification of Proteus and Providence cultures.. J Gen Microbiol 1955 Aug;13(1):155-61.
      pubmed: 13252224doi: 10.1099/00221287-13-1-155google scholar: lookup
    30. KOVACS N. Identification of Pseudomonas pyocyanea by the oxidase reaction.. Nature 1956 Sep 29;178(4535):703.
      pubmed: 13369512doi: 10.1038/178703a0google scholar: lookup

    Citations

    This article has been cited 10 times.
    1. Conley BE, Intile PJ, Bond DR, Gralnick JA. Divergent Nrf Family Proteins and MtrCAB Homologs Facilitate Extracellular Electron Transfer in Aeromonas hydrophila.. Appl Environ Microbiol 2018 Dec 1;84(23).
      doi: 10.1128/AEM.02134-18pubmed: 30266730google scholar: lookup
    2. Romero A, Saraceni PR, Merino S, Figueras A, Tomás JM, Novoa B. The Animal Model Determines the Results of Aeromonas Virulence Factors.. Front Microbiol 2016;7:1574.
      doi: 10.3389/fmicb.2016.01574pubmed: 27757107google scholar: lookup
    3. Igbinosa IH, Igumbor EU, Aghdasi F, Tom M, Okoh AI. Emerging Aeromonas species infections and their significance in public health.. ScientificWorldJournal 2012;2012:625023.
      doi: 10.1100/2012/625023pubmed: 22701365google scholar: lookup
    4. Singh BR, Gulati BR, Virmani N, Chauhan M. Outbreak of Abortions and Infertility in Thoroughbred Mares Associated with Waterborne Aeromonas hydrophila.. Indian J Microbiol 2011 Jun;51(2):212-6.
      doi: 10.1007/s12088-011-0088-3pubmed: 22654167google scholar: lookup
    5. Ceylan E, Berktas M, Ağaoğlu Z. The occurrence and antibiotic resistance of motile Aeromonas in livestock.. Trop Anim Health Prod 2009 Feb;41(2):199-204.
      doi: 10.1007/s11250-008-9175-9pubmed: 18481193google scholar: lookup
    6. Seshadri R, Joseph SW, Chopra AK, Sha J, Shaw J, Graf J, Haft D, Wu M, Ren Q, Rosovitz MJ, Madupu R, Tallon L, Kim M, Jin S, Vuong H, Stine OC, Ali A, Horneman AJ, Heidelberg JF. Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades.. J Bacteriol 2006 Dec;188(23):8272-82.
      doi: 10.1128/JB.00621-06pubmed: 16980456google scholar: lookup
    7. Hathcock TL, Schumacher J, Wright JC, Stringfellow J. The prevalence of Aeromonas species in feces of horses with diarrhea.. J Vet Intern Med 1999 Jul-Aug;13(4):357-60.
      doi: 10.1892/0891-6640(1999)013<0357:tposif>2.3.co;2pubmed: 10449228google scholar: lookup
    8. Ljungh A, Wadström T. Aeromonas and plesiomonas as possible causes of diarrhoea.. Infection 1985 Jul-Aug;13(4):169-73.
      doi: 10.1007/BF01642805pubmed: 3899944google scholar: lookup
    9. Gray SJ, Stickler DJ. Some observations on the faecal carriage of mesophilic Aeromonas species in cows and pigs.. Epidemiol Infect 1989 Dec;103(3):523-37.
      doi: 10.1017/s0950268800030922pubmed: 2606161google scholar: lookup
    10. Gray SJ, Stickler DJ, Bryant TN. The incidence of virulence factors in mesophilic Aeromonas species isolated from farm animals and their environment.. Epidemiol Infect 1990 Oct;105(2):277-94.
      doi: 10.1017/s0950268800047889pubmed: 2209733google scholar: lookup
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