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Journal of veterinary internal medicine2020; 34(6); 2758-2766; doi: 10.1111/jvim.15889

Agreement of 2 electrolyte analyzers for identifying electrolyte and acid-base disorders in sick horses.

Abstract: Use of different analyzers to measure electrolytes in the same horse can lead to different interpretation of acid-base balance when using the simplified strong ion difference (sSID) approach. Objective: Investigate the level of agreement between 2 analyzers in determining electrolytes concentrations, sSID variables, and acid-base disorders in sick horses. Methods: One hundred twenty-four hospitalized horses. Methods: Retrospective study using paired samples. Electrolytes were measured using a Beckman Coulter AU480 Chemistry analyzer (PBMA) and a Nova Biomedical Stat Profile (WBGA), respectively. Calculated sSID variables included strong ion difference, SID ; unmeasured strong ions, USI; and total nonvolatile buffer ion concentration in plasma (A ). Agreement between analyzers was explored using Passing-Bablok regression and Bland-Altman analysis. Kappa (κ) test evaluated the level of agreement between analyzers in detecting acid-base disorders. Results: Methodologic differences were identified in measured Na and Cl and calculated values of SID and USI. Mean bias (95% limits of agreement) for Na , Cl , SID , and USI were: -1.2 mmol/L (-9.2 to 6.8), 4.4 mmol/L (-4.4 to 13), -5.4 mmol/L (-13 to 2), and -6.2 mmol/L (-14 to 1.7), respectively. The intraclass correlation coefficient for SID and USI was .55 (95%CI: -0.2 to 0.8) and .2 (95%CI: -0.15 to 0.48), respectively. There was a poor agreement between analyzers for detection of SID (κ = 0.20, 95%CI, 0.1 to 0.31) or USI abnormalities (κ = -0.04, 95%CI, -0.11 to 0.02). Conclusions: Differences between analyzer methodology in measuring electrolytes led to a poor agreement between the diagnosis of acid-base disorders in sick horses when using the sSID approach.
© 2020 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC on behalf of American College of Veterinary Internal Medicine.
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Publication Date: 2020-09-23 PubMed ID: 32965055PubMed Central: PMC7694849DOI: 10.1111/jvim.15889Google Scholar: Lookup
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Summary

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This research investigates the lack of consistency between two electrolyte analyzers and their determination of electrolyte concentrations and acid-base disorders in ill horses. In particular, it was found that the methodology variations between the analyzers resulted in a discrepancy when diagnosing acid-base disorders using a specific approach.

Research Methodology

  • The research involved a retrospective study with samples taken from 124 hospitalized horses.
  • Interestingly, the researchers adopted a method where they utilised two separate electrolyte analyzers for the experiment; Beckman Coulter AU480 Chemistry analyzer (PBMA) and a Nova Biomedical Stat Profile (WBGA).
  • Through these analyzers, the electrolyte concentration was measured and variables for the simplified strong ion difference (sSID) approach were calculated.
  • This approach considered variables such as the strong ion difference (SID), unmeasured strong ions (USI), and total nonvolatile buffer ion concentration in plasma (A).
  • In order to check for agreement between the results of the two analyzers, Passing-Bablok regression and Bland-Altman analysis were employed.
  • The level of agreement in identifying acid-base abnormalities between the two analyzers was tested via Kappa (κ) test.

Results

  • There were differences identified in the method of measuring the electrolytes Na and Cl, as well as in the calculated values of SID and USI.
  • Furthermore, the intraclass correlation coefficient between SID and USI was as low as .55 and .2 respectively.
  • The lack of agreement between the two analyzers in detecting abnormalities in SID and USI was quite poor (κ = 0.20 for SID and κ = -0.04 for USI).

Conclusion

  • The research concluded that differences in the methodology of the two electrolyte analyzers resulted in a poor correlation in diagnosing acid-base disorders in sick horses when using the sSID method.
  • This lack of consistency can be attributed to how the analyzers measure electrolytes, prompting the need to standardize methods to ensure accurate diagnostic results.

Cite This Article

APA
Gomez DE, Buczinski S, Darby S, Palmisano M, Beatty SSK, Mackay RJ. (2020). Agreement of 2 electrolyte analyzers for identifying electrolyte and acid-base disorders in sick horses. J Vet Intern Med, 34(6), 2758-2766. https://doi.org/10.1111/jvim.15889

Publication

Journal of veterinary internal medicine
ISSN: 1939-1676
NlmUniqueID: 8708660
Country: United States
Language: English
Volume: 34
Issue: 6
Pages: 2758-2766

Researcher Affiliations

Gomez, Diego E
  • Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
  • Department of Clinical Studies, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USA.
Buczinski, Sébastien
  • Département de Sciences Cliniques, Faculté de Médecine Vétérinaire, Université de Montréal, Montréal, Q, Canada.
Darby, Shannon
  • Department of Clinical Studies, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USA.
Palmisano, Megan
  • Department of Clinical Studies, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USA.
Beatty, Sarah S K
  • Department of Comparative, Diagnostic & Population Medicine, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USA.
Mackay, Robert J
  • Department of Clinical Studies, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USA.

MeSH Terms

  • Acid-Base Equilibrium
  • Acid-Base Imbalance / diagnosis
  • Acid-Base Imbalance / veterinary
  • Animals
  • Electrolytes
  • Horse Diseases / diagnosis
  • Horses
  • Retrospective Studies
  • Sodium

Conflict of Interest Statement

Sébastien Buczinski serves as Consulting Editor for Experimental Design and Statistics for the Journal of Veterinary Internal Medicine. He was not involved in review of this manuscript.

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Citations

This article has been cited 1 times.
  1. Coenen MC, Gille L, Eppe J, Casalta H, Bayrou C, Dubreucq P, Frisée V, Moula N, Evrard J, Martinelle L, Sartelet A, Bossaert P, Djebala S. Blood Inflammatory, Hydro-Electrolytes and Acid-Base Changes in Belgian Blue Cows Developing Parietal Fibrinous Peritonitis or Generalised Peritonitis after Caesarean Section. Vet Sci 2022 Mar 14;9(3).
    doi: 10.3390/vetsci9030134pubmed: 35324862google scholar: lookup
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